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Experimental techniques used and results obtained are reported for studies of the effects of simultaneous multiple operating parameters on KO2 canisters in manned space vehicles. Actual experiments using rodents, men, and simulated man, are discussed. Experimental characteristics of single and dual canister (demand) atmosphere composition control systems are analyzed. The best method for experimental definitive Respiratory Quotient matching is described. A new annular screen device for a chemical canister which prevents blocking of the airflow in granular solid chemical bed due to humidity is described. A canister design method was developed to determine basic KO2 canister sizes for atmosphere composition control system tests on a realtime, one-man basis. The dominant role of PCO2 in establishing the CO2 adsorption rate and the O2 generation rate as well as the roles of absolute humidity and catalysts in establishing the O2 generation rate are discussed. A series of comparative tables and curves is presented for O2-CO2 generation and adsorption support levels for one man.