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This book is a complete guide to histopathology techniques for trainees. Beginning with an introduction to tissue examination, the next chapters discuss fixation and fixatives, tissue processing and embedding, decalcification, microtomy and section cutting, and frozen section and cryostat. The following sections cover different staining procedures, immunohistochemistry, and automation in histopathology, concluding with chapters on biological waste management and quality management. Each chapter includes a self-assessment exercise with short notes and answers, and the comprehensive text is further enhanced by nearly 350 clinical photographs, diagrams and tables. Key points Complete guide to histopathology techniques for trainees Provides detail on different staining procedures, immunohistochemistry, and automation Features self-assessment exercises with notes and answers Highly illustrated with clinical photographs, diagrams and tables
This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.
This book provides detailed information on basic and advanced laboratory techniques in histopathology and cytology. It discusses the principles of and offers clear guidance on all routine and special laboratory techniques. In addition, it covers various advanced laboratory techniques, such as immunocytochemistry, flow cytometry, liquid based cytology, polymerase chain reaction, tissue microarray, and molecular technology. Further, the book includes numerous color illustrations, tables and boxes to familiarize the reader with the work of a pathology laboratory. The book is mainly intended for postgraduate students and fellows in pathology as well as practicing pathologists. The book is also relevant for all the laboratory technicians and students of laboratory technology.
本书内容全面,涵盖了组织学技术的方方面面,既包括传统的常用技术,又介绍了近年来新发展的,有较高实用价值的新技术。各章节分别论述了光镜、组织固定、样本处理、冰冻切片、石蜡切片、酶组织学、免疫组化、免疫荧光、组织芯片、原位杂交、荧光原位杂交、激光显微解剖、电镜等技术;对于结缔组织、糖类、脂类、蛋白质、核酸、色素及矿物质、淀粉、神经内分泌颗粒、微体、骨等相关的组绢学技术也予专门章节讲述;另外还涉及了显微镜样品定量、实验室安全及管理,以及人体工程学等相关内容。
In this manual, author has included the molecular techniques, immunohistochemistry, cell blocks, and immunofluorescence along with the conventional techniques. For students' easy understanding; many figures, charts, diagrams and tables have been included. At the same time, the volume of the book has been restricted; so that students do not become overburdened during preparation of examination. [Ed.].
This book provides a concise definition of the fundamental principles and tech-niques involved in histotechnology. A chapter on Laboratory Risk Management emphasizes the importance of instrumentation, quality control and safety precautions in the working place. The methods involved in tissue processing, histologic staining and cytology are discussed. The use of microwave as a non-chemical way of tissue stabilization and fixation, rapid tissue processing, archival material, enzyme histochemistry and molecular biological techniques such as in-situ hybridization and polymerase chain reaction techniques are described. This book also includes a brief course in immunology, covering antigen-antibody reactions, and the required steps in immunostaining.
Handbook of Histopathological and Histochemical Techniques (Including Museum Techniques), Third Edition is a reference guide on techniques used in histology and histochemistry, including museum techniques. This book is comprised of 36 chapters divided into six sections. After giving a brief description of the structure and contents of the cell, this book then turns to the various methods of examination of tissue cells and structures, including the smear method, vital staining, and electron microscopy. The next two sections deal with fixation, decalcification, processing, and section cutting, along with stains, impregnations, and mountants. An exhaustive survey of carbohydrate and protein histochemistry is included. Special procedures such as autoradiography, micro-incineration, and injection techniques are described. The remaining chapters focus on the preparation, mounting, and presentation of museum specimens. The book concludes with an analysis of the microscope. This monograph will be invaluable to all those with an interest in histology and histochemistry, including undergraduate students in the biological and health sciences, pathologists in both the hospital setting and experimental research, and technologists and workers in various research fields.
Histopatology describes the processes and practices that are central to the role of the histopathologist within a functioning diagnostic laboratory, from pre-sampling to diagnosis to laboratory management.
For 40 years, Bancroft's Theory and Practice of Histological Techniques has established itself as the standard reference for histotechnologists and laboratory scientists, as well as histopathologists. With coverage of the full range of histological techniques used in medical laboratories and pathology departments, it provides a strong foundation in all aspects of histological technology – from basic methods of section preparation and staining, to advanced diagnostic techniques such as immunocytochemistry and molecular testing. This revised and updated 8th Edition by Kim S. Suvarna, Christopher Layton, and John D. Bancroft is a one-stop reference for all those involved with histological preparations and applications, from student to highly advanced laboratory professional. - Presents a thorough, up-to-date, and detailed approach to the theory and protocols for preparing cells and tissues for laboratory evaluation, covering topics ranging from basic to advanced - Features new content on automation (computer logging and tracking, sub-sampling and archiving of samples), digital scanning techniques for slides used in remote/telepathology, and specialist molecular techniques. - Provides superb visual guidance through the use of vivid color illustrations, including additional illustrations of diagnostic modalities and techniques. - Contains more summary tables, charts, and boxes throughout for quick reference. - Expert ConsultTM eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, and references from the book on a variety of devices.