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Laser wave mixing is presented as a sensitive absorbance-based detection method for very low activity levels of protease enzymes. Trypsin and proteinase K enzyme activities are analyzed using casein protein covalently labeled with multiple fluorescent BODIPY dye molecules. A preliminary detection limit for trypsin is determined to be 6.34 × 10−14 M or 1.51 pg/mL. For anisotropy studies, wave mixing and fluorescence trends have inverse relationships at the onset of catalysis. Wave mixing is also investigated as a new optical method for the measurement of FRET. The relationship between the wave-mixing signal and FRET is verified using the acceptor/donor pair malachite green and erythrosin B. Resonance energy transfer between a fluorophore and a quencher molecule bound to complimentary oligonucleotide strands is studied to calculate the dye-to-dye distance on a 31-bp curved DNA fragment. The result suggests that there is a direct measurable relationship between the efficiency of resonance energy transfer and the wave-mixing signal. For the first time, wave mixing is applied to the measurement of analyte species in the inductively coupled plasma atomizer. The use of counter-propagating input beams yields sub-Doppler spectral resolution. Nonlinear optical coherence theory is used to predict and study the intensity and hyperfine profiles of atomic populations in the plasma torch. Wavelength modulation and ion line detection in the ICP are investigated for S/N enhancement. Laser wave mixing is also presented as an effective technique for kinetic temperature measurement in an atmospheric-pressure RF inductively coupled plasma using the 4s3P2 2!4p3D33 argon transition probed by a tunable 811.5-nm diode laser. Kinetic temperature measurements are made at five radial steps from the center of the torch and at four different torch heights. The kinetic temperature is determined by simultaneously measuring the line shapes of the sub-Doppler backward phase-conjugate wave-mixing signal and the Doppler-broadened forward-scattering wave-mixing signal.
A comprehensive handbook outlining state-of-the-art analytical techniques used in geomicrobiology, for advanced students, researchers and professional scientists.
Bioinorganic photochemistry is a rapidly evolving field integrating inorganic photochemistry with biological, medical and environmental sciences. The interactions of light with inorganic species in natural systems, and the applications in artificial systems of medical or environmental importance, form the basis of this challenging inter-disciplinary research area. Bioinorganic Photochemistry provides a comprehensive overview of the concepts and reactions fundamental to the field, illustrating important applications in biological, medical and environmental sciences. Topics covered include: Cosmic and environmental photochemistry Photochemistry of biologically relevant nanoassemblies Molecular aspects of photosynthesis Photoinduced electron transfer in biosystems Modern therapeutic strategies in photomedicine The book concludes with an outlook for the future of environmental protection, discussing emerging techniques in the field of pollution abatement, and the potential for bioinorganic photochemistry as a pathway to developing cheap, environmentally friendly sources of energy. Written as an authoritative guide for researchers involved in the development of bioinorganic photochemical processes, Bioinorganic Photochemistry is also accessible to scientists new to the field, and will be a key reference source for advanced courses in inorganic, and bioinorganic chemistry.
This book contains an excellent overview of the status and highlights of brilliant light facilities and their applications in biology, chemistry, medicine, materials and environmental sciences. Overview papers on diverse fields of research by leading experts are accompanied by the highlights in the near and long-term perspectives of brilliant X-Ray photon beam usage for fundamental and applied research.
For centuries, scientists have been fascinated by the role of the Sun in the Earth's climate system. Recent discoveries, outlined in this book, have gradually unveiled a complex picture, in which our variable Sun affects the climate variability via a number of subtle pathways, the implications of which are only now becoming clear. This handbook provides the scientifically curious, from undergraduate students to policy makers with a complete and accessible panorama of our present understanding of the Sun-climate connection. 61 experts from different communities have contributed to it, which reflects the highly multidisciplinary nature of this topic. The handbook is organised as a mosaic of short chapters, each of which addresses a specific aspect, and can be read independently. The reader will learn about the assumptions, the data, the models, and the unknowns behind each mechanism by which solar variability may impact climate variability. None of these mechanisms can adequately explain global warming observed since the 1950s. However, several of them do impact climate variability, in particular on a regional level. This handbook aims at addressing these issues in a factual way, and thereby challenge the reader to sharpen his/her critical thinking in a debate that is frequently distorted by unfounded claims.
In the next 10 to 15 years, chemical engineers have the potential to affect every aspect of American life and promote the scientific and industrial leadership of the United States. Frontiers in Chemical Engineering explores the opportunities available and gives a blueprint for turning a multitude of promising visions into realities. It also examines the likely changes in how chemical engineers will be educated and take their place in the profession, and presents new research opportunities.
Laser Photoionization Spectroscopy discusses the features and the development of photoionization technique. This book explores the progress in the application of lasers, which improve the characteristics of spectroscopic methods. Organized into 12 chapters, this book starts with an overview of the fundamentals of the method for atoms and molecules. This text then examines the photoionization spectroscopy, which is based on the laser resonant excitation of particles into high-lying quantum states that are easy to detect by ionization. Other chapters explain the various basic schemes of multistep excitation, which can be used for resonance photoionization of molecules. This book discusses as well the different applications of the resonance photoionization technique in atomic and molecular spectroscopy. The final chapter considers the two well-known types of microscopy, namely, wave and corpuscular. This book is a valuable resource for chemists, physicists, analysts, and geochemists who are interested in laser spectroscopy techniques to solve nontrivial problems.
Advanced Material Interfaces is a state-of-the-art look at innovative methodologies and strategies adopted for interfaces and their applications. The 13 chapters are written by eminent researchers not only elaborate complex interfaces fashioned of solids, liquids, and gases, but also ensures cross-disciplinary mixture and blends of physics, chemistry, materials science, engineering and life sciences. Advanced interfaces operate fundamental roles in essentially all integrated devices. It is therefore of the utmost urgency to focus on how newly-discovered fundamental constituents and interfacial progressions can be materialized and used for precise purposes. Interfaces are associated in wide multiplicity of application spectrum from chemical catalysis to drug functions and the advancement is funnelled by fine-tuning of our fundamental understanding of the interface effects.
Few books achieve a connection between scientifc theory and real world environmental problems, but this one does. Generous use of color images, exercises, and case studies make it friendly for the classroom or non-mineralogist. Discover crystallography, surface chemistry, mineral-solution equilibria, organic matter, and soil mineral analysis. The book includes a lengthy exploration of world-wide applications of mineralogy in soil taxonomy, tectonics, radionuclides, pesticides, enzymes, and more.
Nanomaterials Characterization Techniques, Volume Two, part of an ongoing series, offers a detailed analysis of the different types of spectroscopic methods currently being used in nanocharacterization. These include, for example, the Raman spectroscopic method for the characterization of carbon nanotubes (CNTs). This book outlines the different kinds of spectroscopic tools being used for the characterization of nanomaterials and discusses under what conditions each should be used. The book is intended to cover all the major spectroscopic techniques for nanocharacterization, making it an important resource for both the academic community at the research level and the industrial community involved in nanomanufacturing. - Explores how spectroscopy and X-ray-based nanocharacterization techniques are applied in modern industry - Analyzes all the major spectroscopy and X-ray-based nanocharacterization techniques, allowing the reader to choose the best for their situation - Presents a method-orientated approach that explains how to successfully use each technique