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Trempealeau County is in the western part of Wisconsin, on the Mississippi River. It is bounded on the east by Jackson County, on the north by Eau Claire County, on the west by Buffalo County, as well as by Winona County across the Mississippi River in Minnesota. The area belongs entirely to the Mississippi system, and is separated into three distinct divisions, the Trempealeau Prairie Region, the Trempealeau Valley Region and the Beef River Region. The county was created Jan. 24, 1854.