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V.1- Protens; v.2.B. Nucleic acids; v.2c- Lipi ds, carbohydrates, stervides.
Methodologies and databases for biochemistry and molecular biology are included in this easy-to-use laboratory reference. Its logical presentation enables the reader to quickly and conveniently locate the information relevant to his or her needs. Featured are tables containing data on amino acids, proteins, nucleosides, nucleotides, and nucleic acids. Also featured are lipids and physical chemical data. Edited by a leading professional in the field, this compact, yet comprehensive bench manual serves as the definitive reference source for your laboratory.
A new edition of the popular introductory textbook for biochemistry and molecular biology. * Contains substantial new material * Contains even more of the clear, colour diagrams Completely up to date. Elimination of inessential material has permitted full coverage of the areas of most current interest as well as coverage of essential basic material. Areas of molecular biology such as cell signalling, cancer molecular biology, protein targeting, proteasomes, immune system, eukaryotic gene control are covered fully but still in a clear student friendly style. This makes the book suitable for the most modern type of courses. WHAT'S NEW New or completely re-written chapters - 2. Enzymes 3. The structure of proteins 4. The cell membrane - a structure depending only on weak forces 13. Strategies for metabolic control and their applications to carbohydrate and fat metabolism 17. Cellular disposal of unwanted molecules 23. Eukaryotic gene transcription and control 24. Protein synthesis, intracellular transport and degradation 25. How are newly synthesised proteins delivered to their correct destinations? - Protein targeting 26. Cell signalling 27. The immune system 30. Molecular biology of cancer 33. The cytoskeleton, molecular motors and intracellular transport There are also several major insertions of new material, and minor editing to the rest of the book. SUPPORT MATERIAL ON THE WEB www.oup.com/elliott (look for the site in August 2000) * There will be a sample chapter in November 2000 so that readers can see the design and content * All the illustrations will be available free for downloading (from March 2001) * A detailed description of the purpose of the book: who it's aimed at and why it was written (from August 2000) * A detailed description of what's new to this edition (from August 2000) PLUS Student's Solutions Manual Instructor's Solutions Manual (tbc)
The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.
Very comprehensive and well-organized Handbook covering fundamental acute and chronic aspects of exercise and training, plus nutrition and ergogenic aids, and application to disease. Incorporates much of the new research that has been generated over the past decade, which has yet to be presented in a text book. The presented chapters comprehensively cover all aspects of exercise biochemistry Brings together an international and exceptional editorial team and contributing authors
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Biochemical kinetics refers to the rate at which a reaction takes place. Kinetic mechanisms have played a major role in defining the metabolic pathways, the mechanistic action of enzymes, and even the processing of genetic material. The Handbook of Biochemical Kinetics provides the "underlying scaffolding" of logic for kinetic approaches to distinguish rival models or mechanisms. The handbook also comments on techniques and their likely limitations and pitfalls, as well as derivations of fundamental rate equations that characterize biochemical processes.Key Features* Over 750 pages devoted to theory and techniques for studying enzymic and metabolic processes* Over 1,500 definitions of kinetic and mechanistic terminology, with key references* Practical advice on experimental design of kinetic experiments* Extended step-by-step methods for deriving rate equations* Over 1,000 enzymes, complete with EC numbers, reactions catalyzed, and references to reviews and/or assay methods* Over 5,000 selected references to kinetic methods appearing in the Methods in Enzymology series* 72-page Wordfinder that allows the reader to search by keywords* Summaries of mechanistic studies on key enzymes and protein systems* Over 250 diagrams, figures, tables, and structures
"The first Lab Ref volume compiled recipes and reference data drawn from a selection of our manuals and was intended to save time and spare frustration." ... "In the same spirit, Lab Ref 2 again assembles in one place a new selection of reference information that should maximize the volume's value in a crowded laboratory environment."--Note.
Coinciding with the first TB therapies to enter clinical trials in 60 years, this is the most comprehensive account of the latest developments in clinical, therapeutic and basic research into the disease, presented by the most prolific of all researchers in the field. Divided into three clearly structured volumes, the first deals with molecular biology and biochemistry of the pathogen, including genetics and genomics, as well as drug design. The second volume covers cell biology, immunology and vaccine development, while the third is devoted to epidemiology and clinical approaches, including drug resistance, veterinary aspects and clinical field trials. With one new infection worldwide every second, this is an essential reference for bacteriologists, immunologists, pathologists and pathophysiologists, molecular and cell biologists, as well as those working in the pharmaceutical industry.
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.