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A scheme has been established to analyze complex organic mixtures of water concentrates. It includes addition of deuterated internal standards to the concentrate, fractionation of the concentrate through a combination of extraction under both acidic and basic conditions, partitioning on a short silica gel column, and finally, analysis of the partitioned fractions as well as the unpartitioned concentrate by glass capillary gas chromatography using a flame ionization detector (GC-FID) and glass capillary gas chromatography coupled to a mass spectrometer (GC-MS). The GC-FID and GC-MS analyses are conducted on two types of wall-coated open-tubular glass capillary GC columns, one containing a nonpolar liquid phase (SP2100) and the other a polar liquid phase (SP1000).