Download Free Genetic Analysis Of The Cell Cycle And Cancer In Zebrafish Book in PDF and EPUB Free Download. You can read online Genetic Analysis Of The Cell Cycle And Cancer In Zebrafish and write the review.

Using HeLa cells, we found that the requirement for SIL in mitotic spindle organization is conserved in mammalian cells. A polyclonal antibody revealed endogenous SIL localizes to the mitotic spindle poles during metaphase. Similar to the defects in the csp mutant zebrafish, two short hairpin RNAs targeted against SIL caused microtubule disorganization in HeLa cells. The combination of results from the zebrafish and mammalian studies show that SIL is required for proper organization of the mitotic spindle.
This volume focuses on defining the unique attributes of using the zebrafish cancer model for discovering important pathways and potential drug targets for the treatment of human cancers. Using the zebrafish model, the volume explores oncogene and tumor suppressor discovery, chemical genetic approaches, genomics, epigenetics, cancer imaging, and cell transplantation. Contributed chapters come from the most prominent laboratories working in this field, which provides a unique perspective on zebrafish models from a wide spectrum of the research community. In addition, the book offers a detailed analysis of the most current research in the area for specific zebrafish cancer models, including T cell leukemia, rhabdomyosarcoma, liver and pancreatic cancer, melanoma, neuroblastoma, germ cell tumors, and malignant peripheral sheath tumors. A chapter is also dedicated to the development and utilization of other piscine models of cancer. The compilation of chapters in the volume culminates into a comprehensive and definitive text on zebrafish and cancer, providing a much needed resource on the powerful attributes of the zebrafish model system.
This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999. - Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls - Illustrates many techiques in full-color - Summarizes the Zebrafish Genome Project
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world's leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade to both experienced researchers and more junior members in the lab. - Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors - Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error
Cell division is controlled by genes that regulate the cell cycle. Here we show that the zebrafish specter (spr) mutant is mutation in the cyclin B1 gene, a gene necessary for the G2 to M transition of the cell cycle. The spr mutation mapped to the cyclin B1 gene. Sequencing showed a transition (C139→T) that caused a nonsense mutation in exon 2 of the cyclin B1 gene. In situ hybridization of cyclin B1 revealed that the mRNA is absent in the mutant embryo by gastrulation. We found that the earliest visible mutant phenotype was a darkening of the head caused by the appearance of apoptotic cells in the brain. In situ hybridization of markers expressed in neural precursors and blood cells showed that, in general, mutant embryos have bigger and fewer cells. In situ hybridization of notch1b and deltaa also showed fewer neural stem cells that give rise to fewer but bigger neuronal precursors. Phospho-histone-3 antibody staining suggested that the cell cycle is delayed in the mutant embryo. The expression of the Dual FUCCI transgene in the live spr mutant embryos confirmed that cells are delayed in the S/G2/early M phase of the cell cycle. CRISPR/Cas9 mediated germline mutagenesis of the wild-type cyclin B1 allele showed that the spr mutant phenotype is a result of a nonfunctional Cyclin B1 gene. We conclude that the spr mutant phenotype is caused by the mutation in the cell cycle gene cyclin B1, an essential regulator of the cell cycle progression from the G2 to M phase of the cell cycle. This then leads to mitotic abnormalities and finally developmental arrest.
This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. - Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls - Summarizes the Zebrafish Genome Project
This volume of Methods in Cell Biology, the first of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. - Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls - Illustrates many techiques in full-color - Summarizes the Zebrafish Genome Project
Authored by leading experts in the field, this book provides the first comprehensive overview of the mechanisms of early patterning and morphogenesis in zebrafish. It summarizes the current knowledge and the key questions for the next decade of research.
An overview of the current systems biology-based knowledge and the experimental approaches for deciphering the biological basis of cancer.