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Two-Dimensional Gel Electrophoresis of Proteins: Methods and Applications reviews current methods and clinical applications of two-dimensional gel electrophoresis of proteins, including the QUEST system, silver staining, and peptide mapping. Two-dimensional gel electrophoresis are applied to the study of diseases ranging from inborn errors of metabolism to human germ-line mutation rates, cancer, and mistranslation in animal and bacterial cells. This volume is organized into three sections encompassing 14 chapters and begins with an overview of the methodology of two-dimensional gel electrophoresis, followed by a discussion of computerized two-dimensional gel electrophoresis, silver staining, immunoblotting, and one- and two-dimensional peptide mapping. In most cases, a step-by-step guide to the techniques is given so that procedures may be easily repeated. A catalog of mouse fibroblast proteins is also given. The chapters that follow focus mainly on applications of two-dimensional gel electrophoresis in areas such as clinical and cancer research, human genetics, protein biosynthesis, and gene expression in plants. The final section presents current protein catalogs of Escherichia coli and human HeLa cells. This book is suitable for young researchers as well as for senior scientists working with a wide variety of problems in molecular and cell biology, basic biochemistry, genetics, and clinical research.
Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.
Viroids and Satellites describes plant diseases and their causal agents while also addressing the economic impact of these diseases. The book discusses various strategies for state-of-the-art methods for the detection and control of pathogens in their infected hosts and provides pivotal information from the discovery of viroids through the analysis of their molecular and biological properties, to viroid pathogenesis, host interactions, and RNA silencing pathways. Students, researchers and regulators will find this to be a comprehensive resource on the topics presented. - Provides coverage of the basic biological properties of disease, along with applied knowledge - Features economic impacts, transmission, geographical distribution, epidemiology, detection, and control within each chapter - Organizes viroid diseases by viroid taxonomy and viroid species
The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
"Electrophoresis in Practice" ist seit mehr als zwei Jahrzehnten das Standardwerk in der Elektrophorese. Die 5. Auflage wurde sorgfältig überarbeitet und beinhaltet nun ein erweitertes Kapitel zu Mikromethoden und der chipgebundenen Elektrophorese.
With the end of the Human Genome Project in sight, the next important step is to determine the function of genes. Proteome Research is an important approach to this study and is the first book to comprehensively cover the application of two-dimensional electrophoresis, the central methodology in proteome research. The state-of-the-art is described in detail and the available detection methods are extensively covered. Sufficient detail is given to allow readers to apply these technologies to their own particular requirements.
This book on protocols in semen biology is a compilation of 20 chapters written by 15 experts from 5 Indian Council of Agricultural Research institutions, focusing on the basics of various procedures in semen biology with applications in animal and other allied sciences The information is presented in simple language with illustrative figures and colour microphotographs, making it understandable for readers of every level. It highlights recent findings, the comparative analysis of assays, protocols, points to ponder, background information and major references, and also compares various assays for evaluating a seminal parameters. The book provides a comprehensive resource for beginners, as well as academics, investigators and scientists of animal semen biology and relevant fields. Further, it offers valuable teaching material.
Since the publication of High-Resolution Electrophorsesis and Immunofixation 2e, there have been ever-increasing advances in the analyses of proteins, by electrophoresis in particular. Protein Electrophoresis in Clinical Diagnosis shows the changes in both techniques and interpretation, presenting a comprehensive review of serum protein techniques,
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.