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Through its clear presentation of the basic concepts, Gel Electrophoresis: Nucleic Acids breaks new ground by describing the principles of the technique without resorting to complicated protocols and recipes.
The first edition, published in 1982, became the standard source of electrophoresis protocols for nucleic acid researchers. This updated edition takes account of the advances made in refining established techniques and details many new techniques, including pulse field electrophoresis, gel retardation analysis, and DNA footprinting. Annotation copyrighted by Book News, Inc., Portland, OR
Most will agree that gel electrophoresis is one of the basic pillars of molecular biology. This coined terminology covers a myriad of gel-based separation approaches that rely mainly on fractionating biomolecules under electrophoretic current based mainly on the molecular weight. In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. We try to keep the contents of the book crisp and comprehensive, and hope that it will receive overwhelming interest and deliver benefits and valuable information to the readers.
Gel electrophoresis of nucleic acids is the one technique that spans the whole range of molecular biology techniques. The combination of its high resolution and versatility of its applications makes it the one method used by all molecular biologists. This book gives clear, step-by-step protocols for all the important techniques from simple analytical separations of nucleic acids to the latest PCR techniques. Hence it will be essential reading for all those working in the area of molecular biology. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.
This book on protocols in semen biology is a compilation of 20 chapters written by 15 experts from 5 Indian Council of Agricultural Research institutions, focusing on the basics of various procedures in semen biology with applications in animal and other allied sciences The information is presented in simple language with illustrative figures and colour microphotographs, making it understandable for readers of every level. It highlights recent findings, the comparative analysis of assays, protocols, points to ponder, background information and major references, and also compares various assays for evaluating a seminal parameters. The book provides a comprehensive resource for beginners, as well as academics, investigators and scientists of animal semen biology and relevant fields. Further, it offers valuable teaching material.
''Useful and timely.'' ---Mutagenesis ''Of considerable value.'' ---Journal of Medical Genetics ''Quite readable....a comprehensive overview....perfectly covers the needs of those researchers who have to decide on the best strategy to identify damage or mutations at the molecular level.'' ---Trends in Cell Biology ''The formats of the presentations are uniform and ample and up-to-date references are provided at the end of each chapter...will be welcomed by postgraduate researchers of all ages and should retain its usefulness for a long time.'' ---Endeavour, 21(4), 1997 This important resource thoroughly reviews a wide range of techniques used in mutagenesis research-ranging from established techniques to recently developed methodologies-based on the polymerase chain reaction. DNA damage analysis, DNA repair assays, and mutation detection are a few of the techniques featured. Chapters present detailed experimental protocols benefiting researchers and students in the fields of toxicology, biotechniques, molecular biology, photobiology, medical genetics, and oncology.
Viroids and Satellites describes plant diseases and their causal agents while also addressing the economic impact of these diseases. The book discusses various strategies for state-of-the-art methods for the detection and control of pathogens in their infected hosts and provides pivotal information from the discovery of viroids through the analysis of their molecular and biological properties, to viroid pathogenesis, host interactions, and RNA silencing pathways. Students, researchers and regulators will find this to be a comprehensive resource on the topics presented. Provides coverage of the basic biological properties of disease, along with applied knowledge Features economic impacts, transmission, geographical distribution, epidemiology, detection, and control within each chapter Organizes viroid diseases by viroid taxonomy and viroid species