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Advances in next-generation sequencing technologies (NGS) are revolutionizing the field of food microbiology. Microbial whole genome sequencing (WGS) can provide identification, characterization, and subtyping of pathogens for epidemiological investigations at a level of precision previously not possible. This allows for connections and source attribution to be inferred between related isolates that may be overlooked by traditional techniques. The archiving and global sharing of genome sequences allow for retrospective analysis of virulence genes, antimicrobial resistance markers, mobile genetic elements and other novel genes. The advent of high-throughput 16S rRNA amplicon sequencing, in combination with the advantages offered by massively parallel second-generation sequencing for metagenomics, enable intensive studies on the microbiomes of food products and the impact of foods on the human microbiome. These studies may one day lead to the development of reliable culture-independent methods for food monitoring and surveillance. Similarly, RNA-seq has provided insights into the transcriptomes and hence the behaviour of bacterial pathogens in food, food processing environments, and in interaction with the host at a resolution previously not achieved through the use of microarrays and/or RT-PCR. The vast un-tapped potential applications of NGS along with its rapidly declining costs, give this technology the ability to contribute significantly to consumer protection, global trade facilitation, and increased food safety and security. Despite the rapid advances, challenges remain. How will NGS data be incorporated into our existing global food safety infrastructure? How will massive NGS data be stored and shared globally? What bioinformatics solutions will be used to analyse and optimise these large data sets? This Research Topic discusses recent advances in the field of food microbiology made possible through the use of NGS.
This volume details methods and procedures used to detect and enumerate bacteria in food. Chapters guide readers through food and beverage matrices, techniques used to enumerate bacteria, mixed bacterial strains (naturally present or inoculated), yeast, viruses, protozoan in distinct food matrices, and freshwater. Authoritative and cutting-edge, Detection and Enumeration of Bacteria, Yeast, Viruses, and Protozoan in Foods and Freshwater aims to provide a basic understanding on detection and enumeration of microorganisms in foods.
Molecular landscape for food safety analysis is rapidly revolutionizing because of high resolution and value added resulting analysis of next-generation sequencing (NGS) approaches. These modern sequencing technologies drive worldwide advancements in food safety and quality. Sequencing Technologies in Microbial Food Safety and Quality reviews several practices in that NGS contributes to foodborne pathogens functional characterization, management and control. This book focuses on potential uses of sequencing technologies in microbial food safety and quality and highlights present challenges in the food industry. Key Features: Application of whole genome sequencing technologies in disease diagnostics, surveillance, transmission, and outbreak investigation in food sector Impact of sequencing tools in the area of food microbiology Recent advances in genomic DNA sequencing of microbial species from single cells Microbial bioinformatics resources for food microbiology High-throughput insertion tracking by deep sequencing for the analysis of food pathogens This book includes contributions from experts who have manipulated sequencing tools in relation to microbial food safety and quality. Presenting comprehensive details about NGS approaches in food science, this book is an updated and reliable reference for food scientists, nutritionists, food product investigators to study and implement the sequencing technologies for developing quality and safe food. This book would also serve as informative resource for food industry officials, government researchers, food science or food nutrition students who seek comprehensive knowledge about the role of emerging sequencing technologies in revolutionizing the food industry.
Foodborne microbial outbreaks are a serious food safety and public health concern worldwide. One of the most challenging issues in food safety is the identification and characterization of foodborne microbial communities, which is a core objective of research by food scientists and food microbiologists. This book reviews the molecular advances in food science related to the safety and quality of food along with recent diagnostic tools for the detection of emerging pathogens based on the food commodities. It presents a wide selection of methods for the identification and characterization of foodborne infectious agents. This book provides an overview of foodborne pathogens, diseases, and outbreaks and then proceeds to delve into techniques for characterizing foodborne pathogens using molecular approaches. It reviews state-of-the-art methods for detecting and tracing foodborne pathogens using next-generation sequencing and whole-genome sequencing for controlling foodborne illnesses as well as the application of microorganisms in food production for preventing foodborne illnesses. The book ends with an overview of management systems and analytical tools for public health protection from foodborne illnesses.
The ever-increasing globalization of the food industry demands new interventions and prevention technologies to improve the safety and quality of food. This multidisciplinary new book presents advanced systems for identifying, analyzing, tracking, and monitoring microbial contaminants in food. Key features: • Highlights emerging and re-emerging foodborne microorganisms and their virulence characteristics • Includes recent approaches for food quality assurance and risk management • Describes the practicality of molecular biology and microbial technologies for effectual control of foodborne infections • Presents a detailed overview of the utilization of recent molecular techniques in food microbiology With expert contributions from experienced academics involved in food microbiology and molecular biology research, this book offers indispensable guidance and a contemporary update of the latest developments in food microbial and molecular biology.
Presenting an up-to-date review of the state-of-the-art and main applications of omics technologies to current hot topics in food sciences, this book is divided into four convenient sections. The first section represents an introduction to the development of foodomics and will provide a general overview of DNA-based and protein-based methods. The second section is focused on the main applications of omics to food safety issues, such as chemical hazards, foodborne pathogens, phages, food authentication or GMO detection. The third section is focused on specific food groups and how omics have revolutionized the investigation of dairy and meat products, seafood, agricultural and fermented food products. Finally, the fourth section is devoted to the link between foodomics and health: hot topics such as nutrimetabolomics, food allergy or probiotics are reviewed here. The book brings together work from top international scientists to produce the most significant academic book for some years on omics and food for a broad audience. It presents unique features not covered so far by other books, such as a detailed description of different strategies and applications of omics techniques to many food sectors and provides a welcome addition to the cutting-edge literature in this area for researchers and professionals in food science and food chemistry.
This second fully revised and extended edition of “Zoonoses - Infections Affecting Humans and Animals” covers the most important pathogens impacting both human and animal public health and debates current developments in this interdisciplinary field from a One Health perspective. Following a "setting" approach, the individual chapters each review zoonoses occurring in a specific group of animals, such as production animals, pets or wildlife, or in a defined ecosystem. A focus is put on zoonoses emerging along the food chain and on antibiotic resistance as an increasing challenge in infectious disease management. Special interest chapters debate non-resolved and currently hotly debated zoonoses, foremost COVID-19, influenza, Crohn/paratuberculosis and chronic botulism, also taking into account the economic and ecological aspects of zoonotic disease outbreaks. This second edition includes brand-new chapters on emerging pathogens such as SARS-CoV-2, chlamydia and helminths, it reviews historic zoonoses, provides additional insights into pathogens of reptiles and highlights significant neglected tropical diseases. This reference work is a must-read for researchers, health professionals and students in Microbiology and Veterinary Medicine. The book’s ambition to spread knowledge on zoonoses and on strategies on how to tackle them complies with the United Nations Sustainable Goals, in particular Goal 3 – Good Health and Well-Being.
Trends in Food Safety and Protection explores the recent developments and ongoing research in the field of food safety and protection. The book covers improvements in the existing techniques and implementation of novel analytical methods for detecting and characterizing foodborne pathogens.
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