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Many fungi and bacteria that associate with plants are potentially harmful and can cause disease, while others enter into mutually beneficial sym bioses. Co-evolution of plants with pathogenic and symbiotic microbes has lead to refined mechanisms of reciprocal recognition, defense and counter defense. Genes in both partners determine and regulate these mechanisms. A detailed understanding of these genes provides basic biological insights as well as a starting point for developing novel methods of crop protection against pathogens. This volume deals with defense-related genes of plants and their regulation as well as with the genes of microbes involved in their interaction with plants. Our discussion begins at the level of populations and addresses the complex interaction of plant and microbial genes in multigenic disease resistance and its significance for crop protection as compared to mono genic resistance (Chap. 1). Although monogenic disease resistance may have its problems in the practice of crop protection, it is appealing to the experimentalist: in the so-called gene-for-gene systems, single genes in the plant and in the pathogen specify the compatibility or incompatibility of an interaction providing an ideal experimental system for studying events at the molecular level (Chaps. 2 and 4). Good progress has been made in identifying viral, bacterial, and fungal genes important in virulence and host range (Chaps. 3-6). An important aspect of plant-microbe interactions is the exchange of chemical signals. Microbes can respond to chemical signals of plant origin.
The infection of plants by Heterodera glycines, commonly known as soybean cyst nematode (SCN), is a serious agricultural problem of worldwide extent. Meanwhile, it provides an excellent experimental model to study basic aspects of how cells function, in particular, during biotic challenge. Heterodera glycines challenges plant cells by initiating, developing and sustaining an interaction that results in the formation of a nurse cell from which the nematode derives nourishment. The presented experiments examine (1) how a cell can be de-differentiated and reprogrammed to perform a much different biological role and (2) how a cell’s immune responses can be engaged or suppressed to accomplish that goal. The observation of alpha soluble N-ethylmaleimide-sensitive factor attachment protein (alpha-SNAP) expression, its location within the rhg1 locus and known involvement in the vesicular transport machinery relating to defense made it a strong candidate for further functional analysis. Functional studies demonstrated that overexpression of alpha-SNAP in the susceptible G. max[Williams 82/PI 518671] genotype that lacks its expression results in the partial suppression of H. glycines infection. This indicated that the vesicles could be delivering cargo to the site of infection to engage a defense response. High levels of expression of a cell wall modifying gene called xyloglucan endotransglycosylase also occur during defense. XTHs associate with vesicles, act in the apoplast outside of the cell, and have a well-known function in cell wall restructuring. These observations indicated that alterations in the cell wall composition of nurse cells could be important for the successful defense response. Overexpression of a G. max xyloglucan endotransglycosylase (Gm-XTH) in the susceptible G. max[Williams 82/PI 518671] genotype resulted in a significant negative effect on H. glycines as well as R. reniformis parasitism. The results, including preliminary experiments on components of the vesicle transport system, identify a potent mechanism employed by plants to defend themselves from two types of plant-parasitic nematodes.
Summarizing the 9th Japan-U.S. seminar on plant-pathogen interactions this book presents cutting-edge research on the application of genomics to the investigation of plant-microbe interactions. Genomic and Genetic Analysis of Plant Parasitism and Defense features papers containing original research on the use of genomics and genome-associated technologies in a variety of pathosystems to explore topics such as mechanisms of pathogen compatibility and incompatibility, host-pathogen signaling and mechanisms of plant disease resistance. Focus is placed on genome-wide analyses and the use of large-scale, high throughput genomic tools in combination with classic genetic tools and resources to decipher the molecular basis of plant?microbe interactions.The wide range of pathogens covered as well as examples of exciting new technologies are sure to be of interest to Plant Pathologists, Microbiologists, Agronomists, Plant Biologists, or anyone interested in plant-microbe interactions.
In the Kingdom of Plants, the emergence of flowers was a crucial step in their ability to colonize a large variety of ecological niches on our planet. The two species Petunia x hybrida and Arabidopsis thaliana represent two major groups of flowering plants. In this work, we have shown that HOMEOBOX genes from the WOX family (Wuschel homeoboxes) are heavily involved in polar organ development (such as leaves and sepals, petals, and carpels at the flower level). The maw mawb double mutant in Petunia displays string-like petals, with consequent disappearance of the floral tube. Moreover, we found that these two genes genetically interact with genes from a different family (the MADS family) in ovule identity (ovules are the structures from which seeds develop). We have also shown that other genes from the WOX family are involved in development of a different kind of structures in Petunia: the trichomes. Trichomes are involved in different tasks, protecting the plant from pathogens or abiotic stress. Thanks to functional genetics studies, we have shown functional genetic recruitment of these WOX genes among different plant organs and among different species. This PhD thesis provides evidence for the importance of the WOX family in Evo-Devo studies. Eventually, we unravelled genetic networks controlled by MAW and MAWB trough RNA-Seq analysis.
Plant proteases are involved in most aspects of plant physiology and development, playing key roles in the generation of signaling molecules and as regulators of essential cellular processes such as cell division and metabolism. They take part in important pathways like protein turnover by the degradation of misfolded proteins and the ubiquitin-proteasome pathway, and they are responsible for post-translational modifications of proteins by proteolysis at highly specific sites. Proteases are also implicated in a great variety of environmentally controlled processes, including mobilization of storage proteins during seed germination, development of seedlings, senescence, programmed cell death and defense mechanisms against pests and pathogens. However, in spite of their importance, little is known about the functions and mode of actions of specific plant proteases. This Research Topic collects contributions covering diverse aspects of plant proteases research.
To overcome stresses such as osmotic shock and pathogen attack, internal signals activate plant defense responses. The F8A24.12 gene from Arabidopsis thaliana encodes the serine/threonine protein kinase with the highest similarity at the structural and functional level to a salt-stress induced gene, Esi47, from the salt tolerant wheatgrass Lophopyrum elongatum . Both genes have a small upstream open reading frame (suORF) in their 5 ' untranslated region (5 ' UTR). This element was previously shown to have a regulatory function in the Esi47 gene. Northern blot analysis revealed that under salt stress both genes have an increased level of transcription. Transgenic Arabidopsis plants were produced carrying reporter gene encoding the enzyme?-glucuronidase (GUS) under the control of the F8A24.12 promoter and 5 ' UTR. GUS histochemical assays revealed that F8A24.12 gene expression occurs throughout the life of the plant, particularly in the root elongation zone, in the vascular tissues of roots and leaves, in the flower and in the petal and sepal abscission zone. GUS fluorometric assays demonstrated that the quantity of GUS protein is induced by methyl jasmonate (MeJA) treatment after 12, 24 and 36 hrs and by salt stress after 24 hrs treatment. These data suggest that the F8A24.12 gene is part of the jasmonic acid signaling pathway. This phytohormone is related to the plant growth and plant defense responses. GUS fluorometric assays were also performed on transgenic Arabidopsis carrying an altered suORF promoter-GUS fusion. Mutation of the suORF obliterated the MeJA and salt responsiveness of the F8A24.12 gene promoter plus 5 ' UTR transgene.