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The use of fluoroscopy in pain treatment allows medications to be injected more precisely into the painful area. Fluoroscopic injection often allows invasive pain management to be performed with more comfort, fewer complications, and better results. This book is a step by step guide to fluoroscopic guided pain management procedures covering craniofacial, spine, peripheral nerves, and joint injections. Beginning with an overview of the technique and pharmacology, each chapter is dedicated to a different section of the body. The guide is formatted as a pocket flip book with ‘quick tabs’ for easy navigation and each X-Ray image is presented with a duplicate image with a colour overlay outlining the anatomy. Authored by experts in the field, led by Texas-based Rudy Garza, this quick reference manual provides clinicians with concise and valuable information on accurate interpretation of imaging and anatomy, and needle guidance. Key points Step by step guide to fluoroscopic guided pain management procedures Presented as a pocket flip book with ‘quick tabs’ for easy navigation Duplicate images with colour overlay outlining anatomy accompany each X-Ray image Authored by experts in the field led by Texas-based Rudy Garza
The chapters in CYTOMETRY MCB volumes, including this 4th Edition, provide comprehensive description of particular cytometric methods and review their applications. Some chapters also describe new instrumentation and provide fundamental information on use of new fluorescent probes and on data analysis. Although the term "edition" suggests the update of earlier volumes, in fact, nearly all chapters of the 4th Edition are devoted to new topics. The authors were invited to present not only technical protocols, such as available in other methodology books that specialize in the protocol format, but also to discuss the aspects of the methodology that generally are not included in the protocols. Many chapters, thus, present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical setting, common traps and pitfalls, problems with data interpretation, comparison with alternative assays, choice of the optimal assay, etc. Some chapters review applications of cytometry and complementary methodologies to particular biological problems or clinical tasks. - Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays - Discusses many new assays developed since the previous edition - Presents recent developments in cytometric intrumentation/technology
It's summer time and the temperature is rising in the small coastal town of Robe, where Astonvale's A-list comes to catch up, dress down and be seen. Professional organiser Celeste Pretty has a summer getaway planned in well-to-do Robe, the 'Hamptons' of Australia. She's staying at the beach 'shack' of her hunky new builder beau, Lenny Muscat. The first week she'll de-clutter the holiday home of fashion designer Sabine Cloud with her assistant, Flip Belmont. Then she'll enjoy loved-up bliss with Lenny. But despite Celeste's penchant for organisation, things have a way of veering off-script. She soon discovers the last person who occupied Sabine's pad - the designer's model daughter, Romy - died from a possible overdose. Plus, Sabine's uber-glam real estate agent seems intent on throwing herself at Lenny. Meanwhile, Flip is distracted by the bad-boy son of a rock star, Blaise Zenith, who some say is responsible for Romy going off the rails. And Celeste thought sunburn and sand in the house were all she'd have to worry about these holidays.
This textbook is a practical guide to the use of small animal imaging in preclinical research that will assist in the choice of imaging modality and contrast agent and in study design, experimental setup, and data evaluation. All established imaging modalities are discussed in detail, with the assistance of numerous informative illustrations. While the focus of the new edition remains on practical basics, it has been updated to encompass a variety of emerging imaging modalities, methods, and applications. Additional useful hints are also supplied on the installation of a small animal unit, study planning, animal handling, and cost-effective performance of small animal imaging. Cross-calibration methods and data postprocessing are considered in depth. This new edition of Small Animal Imaging will be an invaluable aid for researchers, students, and technicians involved in research into and applications of small animal imaging.
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.TOPICS COVERED:* Single and multiphoton confocal microscopy* Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies* Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells* Use of optical probes to detect microorganisms* Raman and confocal raman microspectroscopy* Fluorescence lifetime imaging microscopy* Digital CCD cameras and their application in biological microscopy
In vivo nuclear magnetic and electron spin resonance spectroscopy is concerned, inter alia, with the noninvasive observation of metabolic changes in living systems, including animals and humans. Typically, the physiologi cal (or pathological) state of an organ or tissue is monitored. This multi faceted approach was developed during the 1980s. It is still a research technique, but will undoubtedly become a clinical tool. We are proud to present this volume (the eleventh of our series) in which some of the pioneers in this area summarize their contributions and review related literature. Bolinger and Lenkinski describe a variety of localization methods suitable for clinical applications of NMR spectroscopy. Schleich, Caines, and Rydzewski summarize their contributions to approaches involving off-resonance rotating frame relaxation and critically compare these with other NMR techniques that may yield similar information. Chang and James outline their approach and share their experience with the technical aspects 1 31 of H and P NMR spectroscopy and spatially localized spectroscopy in studies of brain ischemia. Sodium plays an important role in living systems, a key aspect being the large gradient between intra- and extracellular concentrations of sodium that is maintained by a variety of transport mechanisms. Miller and Elgavish give us a comprehensive review of an important research tool in this 23 area- Na NMR spectroscopy as aided by shift reagents.
Introduction to Biological Imaging Discover what biological imaging is able to accomplish in this up-to-date textbook One of the fundamental goals of biology is to understand how living organisms establish and maintain their spatiotemporal organization of the biochemical, cell biological and developmental biology processes that sustain life. Biological systems are inherently complex with a large number of components needed to sustain cellular function. In order to understand any complex system, one must determine its composition by identifying the components it is made of, how each of these components function and carry out their specific task, and how they interact with one another to function together. To grasp the link of such changes to physiological cell and tissue function and/or pathogenesis/disease progression, we need to understand how modifications alter macromolecular function, macromolecular interactions, and/or spatiotemporal distribution and overall supramolecular structural organization. Biological imaging holds the key to understanding spatiotemporal organization, and will thus be increasingly important for the next generations of biological and biochemical researchers. Introduction to Biological Imaging provides the first comprehensive textbook surveying this subject. It elucidates the fundamental principles underlying the capture and production of bioimages, the requirements of image analysis and interpretation, and some key problems and solutions in bioimaging. It includes everything experimental biologists need to incorporate appropriate bioimaging solutions into their work. Introduction to Biological Imaging readers will also find: Coverage of all major types of biological imaging, including medical imaging, cellular imaging, macromolecular imaging, and more Advice on preparing samples for various imaging methods Specific examples in each chapter connecting bioimaging process to the production of real experimental data Introduction to Biological Imaging is a valuable introduction for undergraduate or graduate students in courses relating to bioimaging, as well as scientists and researchers in the biological and medical fields who want a one-stop reference for the full range of imaging techniques.
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
The advent of contrast-enhanced MRA in the early to mid 1990s revolutionized the clinical approach to vascular imaging: an accurate non-invasive imaging modality, not requiring ionizing radiation or potentially nephrotoxic iodinated contrast media, was able to compete with the more hazardous and invasive catheter angiography. Today, MRA is a safe, easy-to-perform procedure routinely used in most imaging centers, and the continued development of faster, more powerful magnets and more effective contrast agents is increasingly helping to overcome many of the early limitations of the technique. Subdivided into three sections (technique, indications and practical applications) and written by internationally renowned experts in the field, this volume stands out in the current literature on MRA by providing: - detailed information on sequence parameters for different magnets and vascular territories for the optimization of the MRA technique; - a broad overview of the principal indications for which contrast-enhanced MR angiography is ideally suited; - the typical MR imaging findings associated with various pathologies; - the appropriateness of contrast-enhanced MRA as compared with other modalities, such as CTA and CEUS; - the potential pitfalls and limitations of the technique in clinical routine. The volume will prove invaluable to radiologists and clinicians wishing to expand, improve, or consolidate their routine approach to vascular MR imaging.