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The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to anyone doing biological or biochemical research. This practical guide to immunotechniques is especially designed to be easily understood by people with little practical experience using antibodies. It clearly presents detailed, easy-to-follow, step-by-step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage. Key Features * Detailed, easy-to-follow, step-by-step protocols * Convenient, easy-to-use format * Extensive practical information * Essential background information * Helpful hints
Cerebral Palsy in Infancy is a thought-provoking book which introduces a new way of thinking on the development and use of interventions. Relevant to current practice, it advocates early, targeted activity that is focused on increasing muscle activation, training basic actions and minimizing (or preventing) mal-adaptive changes to muscle morphology and function. The authors present recent scientific findings in brain science, movement sciences (developmental biomechanics, motor control mechanisms, motor learning, exercise science) and muscle biology. This knowledge provides the rationale for active intervention, underpinning the need for an early referral to appropriate services. The book features methods for promoting relatively intensive physical activity in young infants without placing a burden on parents which include assistive technologies such as robotics, electronic bilateral limb trainers and baby treadmills. Cerebral Palsy in Infancy begins by specifying the guidelines for training and exercise, outlining the rationale for such intervention. It goes on to cover the fundamentals of neuromotor plasticity and the development and negative effects of limited motor activity on brain organization and corticospinal tract development. Neuromuscular adaptations to impairments and inactivity are discussed along with the General Movement assessment that can provide early diagnosis and prognosis, facilitating very early referral from paediatric specialists to training programs. The book ends with a section featuring various methods of training with the emphasis on preventing/minimizing muscle contracture, stimulating biomechanically critical muscle activity and joint movement. An ideal clinical reference for those working to improve the lives of infants suffering from cerebral palsy. CONTRIBUTORS: Adel Abdullah Alhusaini (Saudi Arabia); David I. Anderson (USA); Nicolas Bayle (France); Roslyn Boyd (Australia); Giovanni Cioni (Italy); Diane L. Damiano (USA); Janet Eyre (UK); Linda Fetters (USA); Mary Galea (Australia); Andrew M Gordon (USA); Martin Gough (UK); Richard L Lieber (USA); Jens Bo Nielsen (Denmark); Micah Perez (Australia); Caroline Teulier (France). "This book provides a comprehensive overview of the challenges of motor development and the consequent impact of poor motor function in later childhood for infants with cerebral palsy (CP)."Reviewed by: Oxford Brookes University on behalf of the British Journal of Occupational Therapy, Dec 2014 conceived and edited by Roberta Shepherd with contributions from internationally renowned expert clinicians and researchers discusses new research and new evidence-based treatment interventions shows how to organize very early and intensive physical activity in young infants to stimulate motor development and growth therapies include the specificity of training and exercise, with emphasis on promoting muscle activity and preventing contracture by active instead of passive stretching methods include new interactive technologies in enhancing home-based training sessions carried out by the infant's family extensive referencing in each chapter for further study chapters feature "Annotations" which illustrate scientific findings
Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.
Rapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. It discusses the effective and rapid methods for the diagnosis of virus infections. It addresses the application of fluorescent antibody techniques to the diagnosis and the investigation of virus infections and the assessment of their value both to the clinician and to the virologist. Some of the topics covered in the book are the fluorescence microscopy; nature of fluorescence; filter systems; transmitted light microscopy; interference filters for transmitted light microscopy; lamp centration and alignment of microscope; contrast-fluorescence condensers; photomicrography; and choice of microscope. The culture of viruses for production of antiserum is fully covered. The selection of cell lines or tissues for virus culture is discussed in detail. The text describes in depth the fluorescent antibody staining techniques. The preparation of specimens from respiratory infections is presented completely. A chapter is devoted to the respiratory syncytial virus. Another section focuses on the analysis influenza virus, paramyxoviruses, and picornaviruses. The book can provide useful information to doctors, virologists, students, and researchers.
The Atlas of Sexually Transmitted Diseases and AIDS, 4th Edition, by Drs. Stephen A. Morse, King K. Holmes, Adele A. Moreland, MD, and Ronald C. Ballard, provides you with an exclusive gallery of STD and AIDS images so you can better diagnose and treat these diseases. Approximately 1,100 unique images – most in full color and 30% new to this edition – depict the clinical signs associated with each type of infection. You’ll also find expert guidance on new vaccines, screening techniques, treatment guidelines, and best practices in the field. Get expert advice on the tests available to reach a definitive diagnosis and review therapeutic options, treatment guidelines, prevention strategies, and management of complications. Access appendices on the selection and evaluation of diagnostic tests, quality control, and test technologies. Effectively diagnose all types of STDs and HIV/AIDS with approximately 1,100 images—most in full color and more than 30% new to this edition―that depict the epidemiology as well as the clinical manifestations of these diseases. Effectively utilize new vaccines for HPV and Hepatitis B, new screening tests for Chlamydia, new drugs under development, new treatment guidelines and best practices in HIV screening, and much more.
"Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology's art program enhances students' understanding of concepts through clear and effective illustrations, diagrams, and photographs. Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology."--BC Campus website.
This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.