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by by Professor Professor A. A. G. G. Everson Pearse Pearse The tide of of the Symposium, at at which the the papers which compromise this volume were presented, is ofthe utmost importance. It was not 'Fixation and Tissue Destruction' or 'Fixation 'Fixation and and Loss Loss ofTissue Components', Components', but but 'Fixation and and Tissue Tissue Preservation'. Historical Historical fixatives, fixatives, some some still still with with us us in the field oflight oflight microscopy microscopy after after over over 100 100 years, are are not not less less guilty guilty than than the the new new historical 'fixatives' of of ultrastructural ultrastructural practice practice some some ofwhich of which remove up up to to 60% 60% ofthe of the material material originally present in in the specimen and and convert the remainder into chemically inert products. There There must must be be few few histochemists histochemists who who do not not appreciate appreciate the great need need for for improve improve ments ments in in the the practice practice of of fixation, fixation, but but whence whence can can we we hopefully hopefully expect expect the arrival arrival of such such improvements? improvements ? Apart Apart from from out out ofthe ofthe blue, or revolutionary, revolutionary, advances advances such such as as the advent advent of of glutaralde hyde, there are perhaps three principal sources.
During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.
This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.
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