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Fish kills are graphic evidence of serious problems in a lake or stream. If the kill is related to the presence of toxic chemicals, there may be human health concerns, in addition to the obvious damage to the ecosystem and the fisheries resources. Depending on the cause of a fish kill, legal and economic ramifications may be involved. If the kill is caused by human or corporate actions, litigation is likely to follow, with possible court-awarded damages and assessed costs for cleanup and restoration. This manual is intended to help fisheries biologists and others to prepare for a fish kill investigation.