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To overcome stresses such as osmotic shock and pathogen attack, internal signals activate plant defense responses. The F8A24.12 gene from Arabidopsis thaliana encodes the serine/threonine protein kinase with the highest similarity at the structural and functional level to a salt-stress induced gene, Esi47, from the salt tolerant wheatgrass Lophopyrum elongatum . Both genes have a small upstream open reading frame (suORF) in their 5 ' untranslated region (5 ' UTR). This element was previously shown to have a regulatory function in the Esi47 gene. Northern blot analysis revealed that under salt stress both genes have an increased level of transcription. Transgenic Arabidopsis plants were produced carrying reporter gene encoding the enzyme?-glucuronidase (GUS) under the control of the F8A24.12 promoter and 5 ' UTR. GUS histochemical assays revealed that F8A24.12 gene expression occurs throughout the life of the plant, particularly in the root elongation zone, in the vascular tissues of roots and leaves, in the flower and in the petal and sepal abscission zone. GUS fluorometric assays demonstrated that the quantity of GUS protein is induced by methyl jasmonate (MeJA) treatment after 12, 24 and 36 hrs and by salt stress after 24 hrs treatment. These data suggest that the F8A24.12 gene is part of the jasmonic acid signaling pathway. This phytohormone is related to the plant growth and plant defense responses. GUS fluorometric assays were also performed on transgenic Arabidopsis carrying an altered suORF promoter-GUS fusion. Mutation of the suORF obliterated the MeJA and salt responsiveness of the F8A24.12 gene promoter plus 5 ' UTR transgene.
Researchers in the field of ecological genomics aim to determine how a genome or a population of genomes interacts with its environment across ecological and evolutionary timescales. Ecological genomics is trans-disciplinary by nature. Ecologists have turned to genomics to be able to elucidate the mechanistic bases of the biodiversity their research tries to understand. Genomicists have turned to ecology in order to better explain the functional cellular and molecular variation they observed in their model organisms. We provide an advanced-level book that covers this recent research and proposes future development for this field. A synthesis of the field of ecological genomics emerges from this volume. Ecological Genomics covers a wide array of organisms (microbes, plants and animals) in order to be able to identify central concepts that motivate and derive from recent investigations in different branches of the tree of life. Ecological Genomics covers 3 fields of research that have most benefited from the recent technological and conceptual developments in the field of ecological genomics: the study of life-history evolution and its impact of genome architectures; the study of the genomic bases of phenotypic plasticity and the study of the genomic bases of adaptation and speciation.
Plant neurobiology is a newly emerging field of plant sciences. It covers signalling and communication at all levels of biological organization – from molecules up to ecological communities. In this book, plants are presented as intelligent and social organisms with complex forms of communication and information processing. Authors from diverse backgrounds such as molecular and cellular biology, electrophysiology, as well as ecology treat the most important aspects of plant communication, including the plant immune system, abilities of plants to recognize self, signal transduction, receptors, plant neurotransmitters and plant neurophysiology. Further, plants are able to recognize the identity of herbivores and organize the defence responses accordingly. The similarities in animal and plant neuronal/immune systems are discussed too. All these hidden aspects of plant life and behaviour will stimulate further intense investigations in order to understand the communicative plants in their whole complexity.
The tomato is commercially important throughout the world both for the fresh fruit market and the processed food industries. It is grown in a wide range of climates in the field, under protection in plastic greenhouses and in heated glasshouses. Genetic, physiological and pathological investigations frequently adopt the tomato plant as a convenient subject. Hitherto, much of the information on tomatoes has been fragmented: tomatoes grown in the field and under protection have been considered separately and the more fundamental findings from research have often failed to reach those involved directly or indirectly in commercial crop production. Similarly, the research scientist is often unaware of the problems of commercial crop production and the possible relevance of his work to the crop. This book is an attempt to rectify that situation. By giving a thorough scientific review of all factors influencing tomato production systems, it is hoped that this book will prove useful to students, researchers and commercial producers alike. It gives the basis for the develop ment of improved cultivars, the formulation of strategies for managing pest, disease and disorder problems and the production of high yields of good quality fruit as well as suggesting important areas for scientific initiatives. The extensive bibliographies provide a comprehensive database for tomato researchers. Such a vast subject could not be covered with authority by anyone author.
New ways to improve cereal crops against fungal, bacterial, and viral diseases are covered in this book that was put together by a group of experts. These include genetics, genome editing systems, and nano-biotechnological tools. Cereal crops are mainly the world's leading food crops and feed a large share of the world population. However, external factors, such as pathogens, have often threatened their productivity. Like wheat, rice, maize, oats, barley, millet and storage, etiology, epidemiology, and diseases in cereal crop management. In addition, the importance of crop genetics and genomics in combating pathogens has been discussed. This book offers up-to-date information on new methods, such as the potential of the genome editing system for crop improvement, in particular the CRISPR-Cas system. The current volume also talks about identification, plant breeding, genome editing, and nanotechnology tools that can be used to fight disease in cereal crops. This book is good for students, teachers, and researchers who study biotic stress in cereals, as well as scientists who study nanotechnology, disease resistance, pathogen biology, genome editing, agriculture sciences, and future biotechnology.
This Methods in Molecular Biology book covers topics such as how to image the structure of plant ovules and embryos, tools for establishing cell lineages, methods for studying the totipotency of plant cells, fluorescence-activated cell sorting and more."