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Recent progress on enzyme immobilisation, enzyme production, coenzyme re generation and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. As more progress in research and application of enzymes has been made the lack of an up-to-date overview of enzyme molecular properties has become more appar ent. Therefore, we started the development of an enzyme data information sys tem as part of protein-design activities at GBF. The present book "Enzyme Hand book" represents the printed version of this data bank. In future a computer searchable version will be also available. The enzymes in this Handbook are arranged according to the Enzyme Com mission list of enzymes. Some 3000 "different" enzymes will be covered. Fre quently enzymes with very different properties are included under the same EC number. Although we intend to give a representative overview on the char acteristics and variability of each enzyme the Handbook is not a compendium. The reader will have to go to the primary literature for more detailed information. Naturally it is not possible to cover all the numerous literature references tor each enzyme (for special enzymes up to 40000) if the data representation is to be concise as is intended.
I t is a pleasure to write a few lines to welcome this labour of Iove. I t is always dangeraus to draw sharp divisions between the interests of different scientists. However, in the present stage of progress in enzymology, there are those who are primarily interested in the molecular mechanisms of the reactions of a few selected enzymes, while others are involved in the grand scheme of the chemical metabolism of cells or whole organisms. Fortunately Dr. Barman has had experience in both the molecular and the metabolic aspects of enzymology. He therefore knows the require ments of research workers interested in enzymes from many different points of view. It would be foolish to hope that a handbook of this kind will provide all the information about enzymes which different specialists would wish to find. The author has attempted to help users in the following way. If one Iooks up a particular enzyme one will find all the basic data and a very good Iist of references for more specialized information. Apart from selection of the type of information provided, the author's judgement on the reliability of data is, of course, of critical importance in a handbook. If contradicting published information about some property of an enzyme has to be sorted out, it is often neither possible to teil the whole story nor to give an objective judgement.
Enzymes: Novel Biotechnological Approaches for the Food Industry provides an in-depth background of the most up-to-date scientific research and information related to food biotechnology and offers a wide spectrum of biological applications. This book addresses novel biotechnological approaches for the use of enzymes in the food industry to help readers understand the potential uses of biological applications to advance research. This is an essential resource to researchers and both undergraduate and graduate students in the biotechnological industries. - Provides fundamental and rigorous scientific information on enzymes - Illustrates enzymes as tools to achieve value and quality to a product, either in vitro or in vivo - Presents the most updated knowledge in the area of food biotechnology - Demonstrates novel horizons and potential for the use of enzymes in industrial applications
Today, as the large international genome sequence projects are gaining a great amount of public attention and huge sequence data bases are created it be comes more and more obvious that we are very limited in our ability to access functional data for the gene products - the proteins, in particular for enzymes. Those data are inherently very difficult to collect, interpret and standardize as they are highly distributed among journals from different fields and are often sub ject to experimental conditions. Nevertheless a systematic collection is essential for our interpretation of the genome information and more so for possible appli cations of that knowledge in the fields of medicine, agriculture, etc .. Recent pro gress on enzyme immobilization, enzyme production, enzyme inhibition, coen zyme regeneration and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. It is the functional profile of an enzyme that enables a biologist of physician to analyze a metabolic pathway and its disturbance; it is the substrate specificity of an enzyme which tells an analytical biochemist how to design an assay; it is the stability, specificity and efficiency of an enzyme which determines its usefulness in the biotechnical transformation of a molecule. And the sum of all these data will have to be considered when the designer of artificial biocatalysts has to choose the optimum prototype to start with.
Recent progress on enzyme immobilisation, enzyme production, coenzyme re generation and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. As more progress in research and application of enzymes has been made the lack of an up-to-date overview of enzyme molecular properties has become more appar ent. Therefore, we started the development of an enzyme data information sys tem as part of protein-design activities at GBF. The present book "Enzyme Hand book" represents the printed vers ion of this data bank. In future a computer searchable version will be also available. The enzymes in this Handbook are arranged according to the Enzyme Com mission list of enzymes. Some 3000 "different" enzymes will be covered. Fre quently enzymes with very different properties are included under the same EC number. Although we intend to give a representative overview on the char acteristics and variability of each enzyme the Handbook is not a compendium. The reader will have to go to the primary literature for more detailed information. Naturally it is not possible to cover aII the numerous literature references for each enzyme (for special enzymes up to 40000) if the data representation is to be concise as is intended.
Recent progress in enzyme immobilisation, enzyme production, coenzyme regeneration and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. As more progress in research and application of enzymes has been made the more apparent has become the lack of an up-to-date overview of enzyme molecular properties. The need for such a data bank was also expressed by the EC-task force "Biotechnology and Information". Therefore we started the development of an enzyme data information system as part of protein-design activities at GBF. The present book "Enzyme Handbook" represents the printed version of this data bank. In future it is also planned to make a com puter searchable version available. The enzymes in the Handbook are arranged according to the 1984 Enzyme Commission list of enzymes and later supplements. Some 3000 "different" en zymes are covered. Frequently very different enzymes are included under the same E. C. number. Although we intended to give a representative overview on the molecular variability of each enzyme, the Handbook is not a com pendium. The reader will have to go to the primary literature for more detailed information. Naturally it is not possible to cover all numerous, up to 40 000, literature references for each enzyme if data representation is to be concise as is intended.
Recent progress on enzyme immobilisation, enzyme production, coenzyme re generation and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. As more progress in research and application of enzymes has been made the lack of an up-to-date overview of enzyme molecular properties has become more appar ent. Therefore, we started the development of an enzyme data information sys tem as part of protein-design activities at GBF. The present book "Enzyme Hand book" represents the printed version of this data bank. In future a computer searchable version will be also available. The enzymes in this Handbook are arranged according to the Enzyme Com mission list of enzymes. Some 3000 "different" enzymes will be covered. Fre quently enzymes with very different properties are included under the same EC number. Although we intend to give a representative overview on the char acteristics and variability of each enzyme the Handbook is not a compendium. The reader will have to go to the primary literature for more detailed information. Naturally it is not possible to cover all the numerous literature references for each enzyme (for special enzymes up to 40000) if the data representation is to be concise as is intended.
Today, as the large international genome sequence projects are gaining a great amount of public attention and huge sequence data bases are created it be comes more and more obvious that we are very limited in our ability to access functional data for the gene products -the proteins, in particular for enzymes. Those data are inherently very difficult to collect, interpret and standardize as they are highly distributed among journals from different fields and are often sub ject to experimental conditions. Nevertheless a systematic collection is essential for our interpretation of the genome information and more so for possible appli cations of that knowledge in the fields of medicine, agriculture, etc .. Recent pro gress on enzyme immobilization, enzyme production, enzyme inhibition, coen zyme regeneration and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. It is the functional profile of an enzyme that enables a biologist of physician to analyze a metabolic pathway and its disturbance; it is the substrate specificity of an enzyme which tells an analytical biochemist how to design an assay; it is the stability, specificity and efficiency of an enzyme which determines its usefulness in the biotechnical transformation of a molecule. And the sum of all these data will have to be considered when the designer of artificial biocatalysts has to choose the optimum prototype to start with.
Today, as the large international genome sequence projects are gaining a great amount of public attention and huge sequence data bases are created it be comes more and more obvious that we are very limited in our ability to access functional data for the gene products - the proteins, in particular for enzymes. Those data are inherently very difficult to collect, interpret and standardize as they are highly distributed among journals from different fields and are often sub ject to experimental conditions. Nevertheless a systematic collection is essential for our interpretation of the genome information and more so for possible appli cations of this knowledge in the fields of medicine, agriculture, etc .. Recent pro gress on enzyme immobilization, enzyme production, enzyme inhibition, coen zyme regeneration and enzyme engineering has opened up fascinating new fields for the potential application of enzymes in a large range of different areas. It is the functional profile of an enzyme that enables a biologist or physician to analyse a metabolic pathway and its disturbance; it is the substrate specificity of an enzyme which tells an analytical biochemist how to design an assay; it is the stability, specificity and efficiency of an enzyme which determines its usefulness in the biotechnical transformation of a molecule. And the sum of all these data will have to be considered when the designer of artificial biocatalysts has to choose the optimum prototype to start with.
The Organic Chemistry of Enzyme-Catalyzed Reactions is not a book on enzymes, but rather a book on the general mechanisms involved in chemical reactions involving enzymes. An enzyme is a protein molecule in a plant or animal that causes specific reactions without itself being permanently altered or destroyed. This is a revised edition of a very successful book, which appeals to both academic and industrial markets. - Illustrates the organic mechanism associated with each enzyme-catalyzed reaction - Makes the connection between organic reaction mechanisms and enzyme mechanisms - Compiles the latest information about molecular mechanisms of enzyme reactions - Accompanied by clearly drawn structures, schemes, and figures - Includes an extensive bibliography on enzyme mechanisms covering the last 30 years - Explains how enzymes can accelerate the rates of chemical reactions with high specificity - Provides approaches to the design of inhibitors of enzyme-catalyzed reactions - Categorizes the cofactors that are appropriate for catalyzing different classes of reactions - Shows how chemical enzyme models are used for mechanistic studies - Describes catalytic antibody design and mechanism - Includes problem sets and solutions for each chapter - Written in an informal and didactic style