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Combines fundamental and applied aspects, including preparing novel enzyme systems isolated from nature and using enzymes in a wide variety of industrial processes. A major theme is enzyme technology, which has had a broad impact in biotechnology, synthetic chemistry, pharmaceuticals, and agriculture. Some 120 papers include studies of biosensors for environmental monitoring, fighting nerve-agent chemical weapons with enzyme technology, the kinetics of inactivating enzymes by inert gas bubbling, applying thick-film technology in the mass production of biosensors, developing recombinant bacteria for degrading dibenzothiophene, sorbitol oxidase from microorganisms, improving the stability of soybean and horseradish peroxidases by covalent chemical modification, analyzing glycolysis-relevant compounds in saliva by microbiosensors, and designing and constructing a new nucleozyme. No subject index. Annotation copyrighted by Book News, Inc., Portland, OR
The recent worldwide explosion of interest in enzymes as cata lysts in industrial processes has arisen primarily because of the potential of major innovative advances which have taken place over the last two decades, foremost among these being novel methods of enzyme immobilization and affinity chromatography for rapid enzyme purification. This interest is now being further stimulated by the remarkable commercial success of several enzyme-based industrial processes, particularly the production of high-fructose syrup in the U. S. and amino acid production in Japan. With the initiation of these and other processes, together with the readying for commercial ization of several other enzyme-based operations, interest has ex panded in other areas in which enzymes may playa useful role, particularly in medicine and analytical chemistry. The development of this technology has required the cooperative efforts of practi tioners of several disciplines, primarily chemical engineers, bio chemists and other life scientists. Indeed, from this cooperation is arising the new interdisciplinary field of Enzyme Engineering. To stimulate communication, information exchange and advance ment of knowledge in this new field on an international level the Engineering Foundation, through the efforts of Lemuel B. Wingard, Jr. , initiated in 1971 a series of international conferences on Enzyme Engineering to be held biannually. The first two conferences were held in Henniker, New Hampshire, in the summers of 1971 and 1973, respectively, while the third conference, from which these proceedings derived, was held in August 1975 in Portland, Oregon.
Considerable worldwide interest has arisen in recent years in the controlled use of enzymes as catalysts in industrial processing, analytical chemistry and medical therapy. This interest has genera ted the new interdisciplinary field of Enzyme Engineering, which includes both the scientific and technologic aspects of the produc tion, purification, immobilization, and application of enzymes in a variety of situations and reactor configurations. A series of Engineering Foundation conferences on Enzyme Engineering was initia ted to provide an international forum for the exchange of ideas and information over the entire range of this new field. The outstanding success of the first two conferences attests to the vigor and poten tial of this field to contribute significantly to a better under standing and resolution of some of the major problems faced by man kind. The first conference, which was held August 9-13, 1971, at Henniker, New Hampshire, U. S. A. , aided significantly in molding the several traditional disciplines that interact to form the field of Enzyme Engineering. The conference was highly successful mainly because many of the key scientists and engineers from the several facets of Enzyme Engineering were brought together for the first time at a single residential meeting. The result was an exchange of ideas and "education" of one another in the pertinent principles of the diverse disciplines which contribute to this field. The second conference, held August 5-10, 1973, at Henniker, New Hampshire, U. S. A.
Enzyme Engineering An authoritative and up-to-date discussion of enzyme engineering and its applications In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deliver a robust treatment of enzyme engineering and its applications in various fields such as biotechnology, life science, and synthesis. The book begins with an introduction to different protein engineering techniques, covers topics like gene mutagenesis methods for directed evolution and rational enzyme design. It includes industrial case studies of enzyme engineering with a focus on selectivity and activity. The authors also discuss new and innovative areas in the field, involving machine learning and artificial intelligence. It offers several insightful perspectives on the future of this work. Readers will also find: A thorough introduction to directed evolution and rational design as protein engineering techniques Comprehensive explorations of screening and selection techniques, gene mutagenesis methods in directed evolution, and guidelines for applying gene mutagenesis in organic chemistry, pharmaceutical applications, and biotechnology Practical discussions of protein engineering of enzyme robustness relevant to organic and pharmaceutical chemistry Treatments of artificial enzymes as promiscuous catalysts Various lessons learned from semi-rational and rational directed evolution A transdisciplinary treatise, Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science is perfect for protein engineers, theoreticians, organic, and pharmaceutical chemists as well as transition metal researchers in catalysis and biotechnologists.
In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course. - Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject - Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification - Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series
Metabolic engineering is a rapidly evolving field that is being applied for the optimization of many different industrial processes. In this issue of Advances in Biochemical Engineering/Biotechnology, developments in different areas of metabolic engineering are reviewed. The contributions discuss the application of metabolic engineering in the improvement of yield and productivity - illustrated by amino acid production and the production of novel compounds - in the production of polyketides and extension of the substrate range - and in the engineering of S. cerevisiae for xylose metabolism, and the improvement of a complex biotransformation process.
Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series
The Enzymes, Volume 47, highlights new advances in the field, with this new volume presenting interesting chapters on The Multipurpose Family of Oxidases, Vanillyl alcohol oxidase, Choline oxidases, Aryl alcohol oxidase, D- and L-amino acid oxidases, Sugar oxidases, Phenolic Compounds hydroxylases, Baeyer-Villiger Monooxygenases, Flavin-dependent halogenases, Flavin-dependent dehalogenases, Styrene Monooxygenases, Bacterial luciferases, Cellobiose Dehydrogenases, Prenylated flavoenzymes, Ene-reductases, Flavoenzymes in Biocatalysis. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in The Enzymes series