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Entomopathogenic bacteria (Bacillus thuringiensis and B. sphaericus) are increasingly used as biopesticides to control larval insect populations which are either agricultural or forestry pests and to reduce those which as adults are vectors of severe human diseases. This new book, the first since 1993 to address all aspects of entomopathogenic bacteria, provides undergraduate and graduate students as well as research scientists with a complete, modern view of this important group of bacteria. The authors, chosen for their sustained contributions to the field, cover both fundamental and applied research in this area. The main topics include bacterial ecology and taxonomy, toxin diversity, activity and mode of action, regulation and environment of the genes, safety and ecotoxicology, production and field application of the bacteria, and outbreaks of resistant populations. The book concludes with the most recent data obtained on transgenic biotechnology and addresses environmental impact issues.
This field manual is designed to provide background and instruction on a broad spectrum of techniques and their use in the evaluation of entomopathogens in the field. The second edition provides updated information and includes two additional chapters and 12 new contributors. The intended audience includes researchers, graduate students, practitioners of integrated pest management (IPM), regulators and those conducting environmental impact studies of entomopathogens.
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups.This book will be an essential laboratory reference for insect pathologists.Features include:* Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens* Details of the practical knowledge needed by beginners to apply the techniques* Chapters written by an international group of experts* Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques* Provides extensive supplemental literature and recipes for media, fixatives and stains
This volume provides background theory and practical protocols for bioassays of bacteria, viruses, fungi and nematodes that can be used as biological control agents against insect pests of agricultural and medical importance.
The 38 chapters of this Field Manual provide the tools required for planning experiments with entomopathogens and their implementation in the field. Basic tools include chapters on the theory and practice of microbial control agents, statistical design of experiments, equipment and application strategies. The major pathogen groups are covered in individual chapters (virus, bacteria, protozoa, fungi, nematodes). Subsequent chapters deal with the impact of naturally occurring and introduced exotic pathogens and inundative application of microbial control agents. The largest section of the Manual is composed of 21 chapters on the application and evaluation of entomopathogens in a wide range of agricultural, forest, domestic and aquatic habitats. Mites and slugs broaden the scope of the book. Supplementary techniques and media for follow-up laboratory studies are described. Three final chapters cover the evaluation of Bt transgenic plants, resistance to insect pathogens and strategies to manage it, and guidelines for evaluating the effects of MCAs on nontarget organisms. Readership: Researchers, graduate students, practitioners of integrated pest management, regulators, those doing environmental impact studies. The book is a stand-alone reference, but is also complementary to the laboratory-oriented Manual of Techniques in Insect Pathology and similar comprehensive texts.
This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.
This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.
The main scientists working with enhancing fungal, bacterial, virus and insect biological control agents on different targets present the latest progress in overcoming the barrier of insufficient virulence. This multi-disciplinary group review their own work and that of others, and describe the approaches being used, the successes and the barriers yet to be overcome. There is no up-to-date equivalent work describing biocontrol, let alone enhanced biocontrol.
A comprehensive, edited volume pulling together research on manipulation of the crop microbiome for climate resilient agriculture Microbes for Climate Resilient Agriculture provides a unique collection of data and a holistic view of the subject with quantitative assessment of how agricultural systems will be transformed in coming decades using hidden treasure of microbes. Authored by leaders in the field and edited to ensure conciseness and clarity, it covers a broad range of agriculturally important crops, discusses the impact of climate change on crops, and examines biotechnologically and environmentally relevant microbes. The book encapsulates the understanding of microbial mediated stress management at field level, and will serve as a springboard for novel research findings and new applications in the field. Chapter coverage includes: the role of the phytomicrobiome in maintaining biofuel crop production in a changing climate; the impact of agriculture on soil microbial community composition and diversity in southeast Asia; climate change impact on plant diseases; microalgae; photosynthetic microorganisms and bioenergy prospects; amelioration of abiotic stresses in plants through multi-faceted beneficial microorganisms; role of methylotrophic bacteria in climate change mitigation; conservation agriculture for climate change resilience; archaeal community structure; mycorrhiza-helping plants to navigate environmental stresses; endophytic microorganisms; bacillus thuringiensis; and microbial nanotechnology for climate resilient agriculture. Clear and succinct chapters contributed and edited by leaders in the field Covers microbes' beneficial and detrimental roles in the microbiome, as well as the functions they perform under stress Discusses the crop microbiome, nutrient cycling microbes, endophytes, mycorrhizae, and various pests and diseases, and their roles in sustainable farming Places research in larger context of climate change's effect on global agriculture Microbes for Climate Resilient Agriculture is an important text for scientists and researchers studying microbiology, biotechnology, environmental biology, agronomy, plant physiology, and plant protection.
Principles of Insect Pathology, a text written from a pathological viewpoint, is intended for graduate-level students and researchers with a limited background in microbiology and in insect diseases. The book explains the importance of insect diseases and illuminates the complexity and diversity of insect-microbe relationships. Principles of Insect Pathology combines the disciplines of microbiology (virology, bacteriology, mycology, protozoology), pathology, and immunology within the context of the insect host, providing a format which is understandable to entomologists, microbiologists, and comparative pathologists.