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This volume provides the first cohesive and authoritative account of this important field. The book will be of interest to protozoologists, protistologists, evolutionary biologists, and ecologists dealing with aquatic or soil ecosystems.
Handbook of Methods in Aquatic Microbial Ecology is the first comprehensive compilation of 85 fundamental methods in modern aquatic microbial ecology. Each method is presented in a detailed, step-by-step format that allows readers to adopt new methods with little difficulty. The methods represent the state of the art, and many have become standard procedures in microbial research and environmental assessment. The book also presents practical advice on how to apply the methods. It will be an indispensable reference for marine and freshwater research laboratories, environmental assessment laboratories, and industrial research labs concerned with microbial measurements in water.
The term "zooplankton" describes the community of floating, often microscopic, animals that inhabit aquatic environments. Being near the base of the food chain, they serve as food for larger animals, such as fish. The ICES (International Council for the Exploration of the Sea) Zooplankton Methodology Manual provides comprehensive coverage of modern techniques in zooplankton ecology written by a group of international experts. Chapters include sampling, acoustic and optical methods, estimation of feeding, growth, reproduction and metabolism, and up-to-date treatment of population genetics and modeling. This book will be a key reference work for marine scientists throughout the world. Sampling and experimental design Collecting zooplankton Techniques for assessing biomass and abundance Protozooplankton enumeration and biomass estimation New optical and acoustic techniques for estimating zooplankton biomass and abundance Methods for measuring zooplankton feeding, growth, reproduction and metabolism Population genetic analysis of zooplankton Modelling zooplankton dynamics This unique and comprehensive reference work will be essential reading for marine and freshwater research scientists and graduates entering the field.
This book presents a compendium of molecular biology applications for the study of aquatic community ecology. The collection presents the diversity of approaches that have been used, and provides future directions for the study of `molecular ecology' of aquatic communities, from viruses to fish, and in aquatic systems ranging from freshwater streams and lakes to estuaries and oceans. This collection of papers will provide a useful text and resource for upper-level undergraduate and graduate students in ecology, as well as for the researcher and educator.
Biodiversity of Fungi is essential for anyone collecting and/or monitoring any fungi. Fascinating and beautiful, fungi are vital components of nearly all ecosystems and impact human health and our economy in a myriad of ways. Standardized methods for documenting diversity and distribution have been lacking. A wealth of information, especially regrading sampling protocols, compiled by an international team of fungal biologists, make Biodiversity of Fungi an incredible and fundamental resource for the study of organismal biodiversity. Chapters cover everything from what is a fungus, to maintaining and organizing a permanent study collection with associated databases; from protocols for sampling slime molds to insect associated fungi; from fungi growing on and in animals and plants to mushrooms and truffles. The chapters are arranged both ecologically and by sampling method rather than by taxonomic group for ease of use. The information presented here is intended for everyone interested in fungi, anyone who needs tools to study them in nature including naturalists, land managers, ecologists, mycologists, and even citizen scientists and sophiscated amateurs. Covers all groups of fungi - from molds to mushrooms, even slime molds Describes sampling protocols for many groups of fungi Arranged by sampling method and ecology to coincide with users needs Beautifully illustrated to document the range of fungi treated and techniques discussed Natural history data are provided for each group of fungi to enable users to modify suggested protocols to meet their needs
Readers familiar with the first three editions of Ecology and Classification of North American Freshwater Invertebrates (edited by J.H. Thorp and A.P. Covich) will welcome the comprehensive revision and expansion of that trusted professional reference manual and educational textbook from a single North American tome into a developing multi-volume series covering inland water invertebrates of the world. The series entitled Thorp and Covich’s Freshwater Invertebrates (edited by J.H. Thorp) begins with the current Volume I: Ecology and General Biology (edited by J.H. Thorp and D.C. Rogers), which is designed as a companion volume for the remaining books in the series. Those following volumes provide taxonomic coverage for specific zoogeographic regions of the world, starting with Keys to Nearctic Fauna (Vol. II) and Keys to Palaearctic Fauna (Vol. III). Volume I maintains the ecological and general biological focus of the previous editions but now expands coverage globally in all chapters, includes more taxonomic groups (e.g., chapters on individual insect orders), and covers additional functional topics such as invasive species, economic impacts, and functional ecology. As in previous editions, the 4th edition of Ecology and Classification of North American Freshwater Invertebrates is designed for use by professionals in universities, government agencies, and private companies as well as by undergraduate and graduate students. Global coverage of aquatic invertebrate ecology Discussions on invertebrate ecology, phylogeny, and general biology written by international experts for each group Separate chapters on invasive species and economic impacts and uses of invertebrates Eight additional chapters on insect orders and a chapter on freshwater millipedes Four new chapters on collecting and culturing techniques, ecology of invasive species, economic impacts, and ecological function of invertebrates Overall expansion of ecology and general biology and a shift of the even more detailed taxonomic keys to other volumes in the projected 9-volume series Identification keys to lower taxonomic levels
Sequences of small subunit (Ss) and large subunit (Ls) ribosomal RNA genes (rDNA) from the marine dinoflagellates Alexandrium tamarense, A. catenella, A. fundyense, A. affine, A. minutum, A. lusitanicum and A. andersoni were compared to assess the organisms' relationships. Cultures represent isolates from North America, Western Europe, Thailand, Japan, Australia and the ballast water of several cargo vessels, and include both toxic and non-toxic strains. An emphasis was placed on the A. tamarense/catenella/fundyense "species complex," a group of morphotypically-similar organisms found in many regions of the world. Two distinct SsrRNA genes, termed the "A gene" and the "B gene," were found in a toxic A. fundyense isolated from eastern North America. The B gene is considered to be a pseudogene. A restriction fragment length polymorphism (RFLP) assay developed to detect the A and B genes revealed five distinct groups of Alexandrium isolates. Three subdivide the A. tamarense/catenella/fundyense complex, but do not correlate with morphospecies designations. The two remaining groups are associated with cultures that clearly differ morphologically from the A. tamarense/ catenella/fundyense group: the fourth group consists of A. affine isolates, and the fifth group is represented by A. minutum, A.lusitanicum and A. andersoni. The B gene was only found in A. tamarense/catenella/ fundyense, but not in all members of this species complex. The B gene is not uniformly distributed among global populations of Alexandrium. All A. tamarense/catenella/fundyense isolates from North America harbor this gene, but it has also been found in some A. tamarense from scattered locations in Japan, as well as in A. tamarense from the ballast water of one cargo vessel which was on a defined run from Japan to Australia. The B gene may be endemic to North American populations of A. tamarense/catenella/fundyense. It is possible that in the recent past North American A. tamarense were introduced to Japanese waters, and cysts of these organisms have been transported from Japan to Australia. A subset of isolates examined using the the RFLP assay were also compared by cloning and sequencing a fragment of their LsrDNA. Eight major classes of LsrDNA sequences, termed "ribotypes," were identified. Five ribotypes subdivide members of the A. tamarense/catenella!fundyense complex; all isolates containing the B gene cluster as one ribotype. The three remaining ribotypes are typified by: 1) A. affine; 2) A. minutum and A. lusitanicum; and, 3) A. andersoni. LsrDNAs from A. minutum and A. lusitanicum are indistinguishable. A. minutum!lusitanicum/andersoni may represent another Alexandrium species complex, analogous to the A. tamarense/catenella/fundyense group. An organisms' ability to produce toxin appears to be correlated with its LsrDNA phylogenetic lineage. Ribotypes ascribed by the LsrDNA sequences are in complete agreement with, and offer a finer-scale resolution of, groups defined by SsrDNA restriction patterns. The SsrDNA RFLP groups and LsrDNA ribotypes are useful species- and population-specific markers. Alexandrium tamarense/catenella/fundyense exist as geneticallydistinct "strains" (populations), not three genetically-distinct species: representatives collected from the same geographic region appear the most similar, regardless of morphotype, whereas those from geographicallyseparated populations are more divergent even when the same morphospecies are compared. Contrary to this general pattern, A. tamarense/catenella from Japan were found to be exceptionally heterogeneous. Ballast water samples show that viable cysts (resting spores) of toxigenic A. tamarense/catenella are being discharged into Australian ports from multiple, genetically-distinct source populations. The rDNA sequences were also used to test theories accounting for the evolution and global dispersal of A. tamarense/catenella/fundyense. Results suggest a monophyletic radiation of these organisms from a common ancestor that included, or gave rise to, multiple morphotypes. Populations appear to have diverged as a result of vicariance (geographic isolation). The co-occurrence of genetically-distinct strains of these organisms is an indication of dispersal. An example of this is seen in Japan where an introduction of North American A. tamarense appears likely. Determining the timing of dispersal events is problematic if based strictly on rDNA sequence similarities, since these molecules undergo change on a scale of millions of years
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