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As membrane trafficking research has expanded over the past thirty years, a remarkable convergence of information has been gained by using genetic approaches in yeast cells with biochemical approaches in mammalian cells. This book reflects these advances by devoting one section of the book to yeast cells and the other to mammalian cells, with each section providing both classic and cutting-edge techniques to study macromolecular transport across the membranes.
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
Cell surface membranes have long been characterized as two-dimensional fluids whose mobile components are randomized by diffusion in the plane of the membrane bilayer. Recent research has indicated that cell surface membranes are highly organized and ordered and that important functional units of membranes appear as arrays of interacting molecules rather than as single, freely diffusing molecules. Mobility and Proximity in Biological Membranes provides an overview of the results obtained from biophysical methods for probing the organization of cell surface membranes. These results are presented in the context of detailed treatments of the theory and the technical demands of each of the methods. The book describes a versatile and easily applied mode for investigating molecular proximities in plasma membranes in a flow cytometer. Its analysis of lipid fluidity and viscosity of membranes and the rotational mobility of proteins offers intimate insight into the physical chemistry of biological membranes. The electrophysiology of lymphocytes is presented with focus on its importance in different diseases. New techniques are described, and new data, new possibilities, and future trends are presented by world experts. This book's chapters can serve both as guides to the existing literature and as starting points for new experiments and approaches associated with problems in membrane function.
This book covers the past, present and future of the intra-cellular trafficking field, which has made a quantum leap in the last few decades. It details how the field has developed and evolved as well as examines future directions.
"Ypt/Rab GTPases form the largest branch of the Ras-related small GTPase superfamily and regulate intracellular membrane trafficking in all eukaryotes. Since their discovery over two decades ago, a wealth of information has accumulated about the roles that"
"Yet another cell and molecular biology book? At the very least, you would think that if I was going to write a textbook, I should write one in an area that really needs one instead of a subject that already has multiple excellent and definitive books. So, why write this book, then? First, it's a course that I have enjoyed teaching for many years, so I am very familiar with what a student really needs to take away from this class within the time constraints of a semester. Second, because it is a course that many students take, there is a greater opportunity to make an impact on more students' pocketbooks than if I were to start off writing a book for a highly specialized upper- level course. And finally, it was fun to research and write, and can be revised easily for inclusion as part of our next textbook, High School Biology."--Open Textbook Library.
Membrane proteins are essential determinants of many biological processes in plants. They function in metabolic processes, signal transduction, transport of small molecules and polymers across endo- and plasma membranes, and intercompartmental trafficking of proteins, lipids, and cell wall components. During these integrative processes, dynamic interactions of membrane proteins with other membrane or soluble components are thought to provide a high degree of flexibility that usually characterizes higher plants. This concept is supported by the recent release of a first, partial Arabidopsis interactome by the Arabidopsis Interactome Mapping Consortium (http://www.sciencemag.org/content/333/6042/601.full.htm). The Arabidopsis interactome reveals a strong enrichment of a few network communities, including those for transmembrane transport and vesicle trafficking. Strikingly, the large transmembrane transport community shares a high amount of proteins with the vesicle trafficking community suggesting a strong physical and functional overlap and interaction.
The peripheral membrane proteins transduce the extracellular signaling into the cells. The peripheral membrane proteins include the G protein-coupled receptors, receptor tyrosine kinases, channels and transporters. The signals, which are transduced via the peripheral membrane proteins, activate several pathways including G protein signaling, MAPK signaling, TNF signaling, TGF? signaling, Wnt signaling and Hedgehog signaling. The peripheral membrane proteins transduce the signaling from the extracellular ligands into the cells. This book intends to provide a comprehensive overview of the features and signaling of peripheral membrane proteins, which includes the molecular structure and interaction. The insights into membrane proteins associated with diseases and therapeutics and the effects of drugs and chemicals are also discussed in this book.