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Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function. - Timely contribution that describes a rapidly changing field - Leading researchers in the field - Broad coverage: Instrumentation, basic theory, data analysis, and applications
Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function. - Timely contribution that describes a rapidly changing field - Leading researchers in the field - Broad coverage: Instrumentation, basic theory, data analysis, and applications
Advances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Magnetic Resonance Characterization of Physiologically Important Metal Ion Binding Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin labels for the study of higher-order DNA G-quadruplex structures, Orthogonal spin labeling and membrane proteins: increasing the information content and going towards in cell applications, Spectroscopic investigation of mono- and di-Mn-containing centers in biochemistry with an emphasis on application of paramagnetic resonance techniques, and more. Additional chapters cover In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance, an Update, EPR contributions to understanding molybdenum-containing enzymes, EPR spectroscopy of Type I reaction centers, Characterization of a substrate-derived radical in the NosN reaction during the biosynthesis of nosiheptide, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology series - Includes the latest information on Advances in Biomolecular EPR
Basic theory, applications, and recent trends in analytical techniques used in crude oil and related products analysis This book covers the application of different spectroscopic methods to characterize crude oil and related products. Its topics are presented in a pedagogical manner so that those new to the subject can better understand the content. The book begins by familiarizing the reader with the rheological characterization of crude oil and related products. Subsequent chapters are directed towards the current trends of different spectroscopic methods for the characterization of crude oil. Analytical Characterization Methods for Crude Oil and Related Products features chapters on: optical interrogation of petroleum asphaltenes (myths and reality); ESR characterization of organic free radicals in petroleum products; high-field, pulsed, and double resonance studies of crude oils and their derivatives; NMR spectroscopy in bitumen characterization; applications of Raman spectroscopy in crude oil and bitumen characterization; and more. Uses a bottom-up approach—starting from the basic theory of the technique followed by its applications and recent trends in crude oil analysis Includes informative content so as to take a technician to the level of using a particular analytical method Covers relevany information so as to enable a manager in the industry to make purchasing decisions Analytical Characterization Methods for Crude Oil and Related Products is aimed at researchers in academia as well as technicians and developers of new analytical methods in the oil industry and related areas. It will also be of interest to professionals, scientists, and graduate students in analytical sciences dealing with oil and environmental analysis.
Written by a pioneer in the development of spin labeling in biophysics, this expert book covers the fundamentals of nitroxide spin labeling through cutting-edge applications in chemistry, physics, materials science, molecular biology, and biomedicine. Nitroxides have earned their place as one of the most popular organic paramagnets due to their suitability as inhibitors of oxidative processes, as a means to polarize magnetic nuclei, and, in molecular biology, as probes and labels to understand molecular structures and dynamics AS DRAGS FOR CANCER AND OTHER DISEASES. Beginning with an overview of the basic methodology and nitroxides’ 145-year history, this book equips students with necessary background and techniques to undertake original research and industry work in this growing field.
This unique, self-contained resource is the first volume on electron paramagnetic resonance (EPR) spectroscopy in the eMagRes Handbook series. The 27 chapters cover the theoretical principles, the common experimental techniques, and many important application areas of modern EPR spectroscopy. EPR Spectroscopy: Fundamentals and Methods is presented in four major parts: A: Fundamental Theory, B: Basic Techniques and Instrumentation, C: High-Resolution Pulse Techniques, and D: Special Techniques. The first part of the book gives the reader an introduction to basic continuous-wave (CW) EPR and an overview of the different magnetic interactions that can be determined by EPR spectroscopy, their associated theoretical description, and their information content. The second provides the basics of the various EPR techniques, including pulse EPR, and EPR imaging, along with the associated instrumentation. Parts C and D builds on parts A and B and offer introductory accounts of a wide range of modern advanced EPR techniques, with examples of applications. The last two parts presents most of the new advances that do not appear in most of the classical EPR textbooks that focus on CW EPR. EPR Spectroscopy: Fundamentals and Methods contains, in concise form, all the material needed to understand state-of-the-art EPR spectroscopy at the graduate school/research level, whilst the editors have ensured that it presents the topic at a level accessible to newcomers to the field and others who want to know its range of application and how to apply it.
Amino Acids, Peptides and Proteins comprises a comprehensive and critical review of significant developments at the biology and chemistry interface. Compiled by leading researchers in their subject, this volume incorporates current trends and emerging areas in topics such as magnetic resonance studies of membrane active peptides, proteins and peptides for the diagnosis and therapy of Leishmania donovani parasite infections and advances in the design of ligands interacting with proteases causing infectious respiratory syndrome. Appealing broadly to researchers in academia and industry, it will be of great benefit to any researcher wanting a succinct reference on developments in this area now and looking to the future.
Who would have thought preparations for a March 2014 Sacred Libation Ceremony honoring one-hundred forty-eight African American women lynched in America would result in the observation melanin is worth more than gold? Dr. Frances Cress Welsing first told us the chemical melanin is produced through a process known as melanogenesis upon introduction of the chemical tyrosine to the enzyme tyrosinase. Melanin is found in such diverse places as bird feathers, animal fur, reptile scales, microorganisms, cephalopod ink, mushrooms and even fossils. Additionally, melanin is found in the hair, skin and eyes of people. Melanin is subjected to intense scientific scrutiny. Nevertheless, the highly educated people studying it had no idea melanin is worth more than gold. In June 2014 a post to the Keyamsha the Awakening blog openly declared melanin was worth $353 a gram and $300 a gram more than gold. Shortly afterwards, hoaxers began bombarding the blog with comments claiming "melanin thieves" were harvesting melanin from Black people. The hoax was easily falsified. The hoaxers made certain to never mention the company selling melanin extracted from the ink of sepia officinalis, the common cuttlefish. During the intervening years the melanin thieves hoaxers persisted in their efforts. In March 2017, the melanin thieves hoaxers launched a "Melanin Twitter bomb" involving the dollar value of melanin after publicity of black women and girls missing in Washington, D.C. was released. Their actions exposed a frailty in their psyche. They also reveal it is possible to wipe out false information involving melanin and take the melanin challenged inferiority complex (aka racism/white supremacy) along for the ride. It then became clear the time had come to enter the fray and dispel the myths about melanin. This book completely obliterates the false narrative of melanin. Perceiving facts from a melanin-centered perspective bestows upon us an expanded awareness of the world and our place in it. It helps provide the average person a means to immerse themselves in melaninology and emerge a "melaninologist." Essentially, we get to know ourselves. To date no other path for the public to independently verify, or falsify, outlandish claims regarding melanin being worth more than gold on their own without any "guru" to guide them have been made available. At this writing, melanin is worth over $395 a gram more than gold. In ancient Kemet (misnomered Egypt) such words were known as hekau or words of power with the ability to heal. For nearly one-hundred years, since August 13, 1920, melanin put the "B" in R.B.G. and the "Black" in Red, Black and Green as the flag of Africans, at home and abroad.. Those are hekau, also. Our Blood, Our Melanin and Africa unites us. More hekau. The Afro, official currency of the United States of Africa, also known as the African Union, is worth $2.22. We are swathed in hekau to such an extent Mchakato Wa Uponyaji (Swahili for the process of healing) has begun. All of which indicates we are living in a new era: the era when all the generations of man can be called blessed on a planet that works for everyone. This era demands we convene the Ubuntu Convention. That plebiscite sets the stage for drafting the Ubuntu Declaration. In emulating the success of the August 1920 Universal Negro Improvement Association convention, we deliberately create the world where we intend to live. The revival of the U.N.I.A. with 12 million dues-paying card-carrying members positions the organization to have a treasury flush with over $400 million liquid. All of which represents a quantum shift in awareness, perception and power underway as you read this. Through our own actions we bring about the total, complete, and absolute Redemption of Africa for all time.
This book describes the methods of analysis and determination of oxidants and oxidative stress in biological systems. Reviews and protocols on select methods of analysis of ROS, RNS, oxygen, redox status, and oxidative stress in biological systems are described in detail. It is an essential resource for both novices and experts in the field of oxidant and oxidative stress biology.