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Electromanipulation of Cells is the first comprehensive, balanced overview of this dynamic discipline. Edited by leading authorities in the field, the book surveys state-of-the-art research as well as recent practical applications of electric field technologies.
This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.
"WHAT DOES NOT NEED TO BE BIG, WILL BE SMALL", a word by an engineer at a recent conference on chips technology. This sentence is particularly true for chemistry. Microfabrication technology emerged from microelectronics into areas like mechanics and now chemistry and biology. The engineering of micron and submicron sized features on the surface of silicon, glass and polymers opens a whole new world. Micromotors smaller than human hair have been fabricated and they work fine. It is the declared goal of the authors to bring these different worlds together in this volume. Authors have been carefully chosen to guarantee for the quality of the contents. An engineer, a chemist or a biologist will find new impulses from the various chapters in this book.
This volume is volume entirely dedicated to microfabricated cell-based systems. It will provide readers with a quick introduction to the field as well as with a variety of specific examples of such Lab-on-Chip systems for cellomics applications. It will give investigators inspiration for innovative research topics, whereas end users will be surprised about the wide variety of new and exciting applications.
Several micro- and nanomanipulation techniques have emerged in recent decades thanks to advances in micro- and nanofabrication. For instance, the atomic force microscope (AFM) uses a nano-sized tip to image, push, pull, cut, and indent biological material in air, liquid, or vacuum. Using micro- and nanofabrication techniques, scientists can make manipulation tools, such as microgrippers and nanotweezers, on the same length scale as the biological samples. Micro and Nano Techniques for the Handling of Biological Samples reviews the different techniques available to manipulate and integrate biological materials in a controlled manner, either by sliding them along a surface (2-D manipulation), or by gripping and moving them to a new position (3-D manipulation). The advantages and drawbacks are mentioned together with examples that reflect the state-of-the-art in manipulation techniques for biological samples. Thanks to the advances in micro- and nanomanipulation techniques, the integration of biomaterials with physical transducers has been possible, giving rise to new and highly sensitive biosensing devices. Although great progress has been made, challenges are still present. To understand the complex interactions between and inside biological samples, scientists will always be working on improving technologies to manipulate, transport, sort, and integrate samples in different environments. Balanced between simplicity for the beginner and hardcore theory for the more advanced readers, this book is the ideal launching point for sharpening the scientific tools required to address these challenges.
The Fifth International Conference on Micro Total Analysis Systems, also known as JlTAS 2001, will highlight the latest exciting events in the world ofminiaturized devices and systems for performing chemical and biochemical experimentation This conference has become mandatory for those of us working in this field as it is indeed helping to define our discipline. We are grateful to the people of the MESA Research Institute of the University of Twente, particularly Piet Bergveld and Albert van den Berg, for starting this meeting in 1994. Their original intention was for the JlTAS meeting to be a small informal workshop. This workshop flavor was sustained through the second meeting held in Basel in 1996, but already in 1998 at the third meeting in Banff it was clear that the "workshop" had become a conference with 420 attendees. It was due to this clearly growing interest in microchemical systems that it was decided we should consider gradually moving toward an annual format and prepare for the possibility that the meeting would increase in popularity. Albert van den Berg was still yearning for a workshop at the JlTAS 2000 meeting and planned a single session format. Again there was a large increase in submitted abstracts (more than 230 total) and a further increase in attendance. The JlTAS steering committee again agreed that we would have to prepare to address the demand the meeting was receiving.
A manual that details the techniques of electrofusion and electroporation by researchers who were the first to show that the platelet membrane glycoproteins GP IIb and GP IIIa are associated in a complex which triggered interest in electrofusion.
This book is a printed edition of the Special Issue "Micro/Nano-Chip Electrokinetics" that was published in Micromachines
MEMS technology and applications have grown at a tremendous pace, while structural dimensions have grown smaller and smaller, reaching down even to the molecular level. With this movement have come new types of applications and rapid advances in the technologies and techniques needed to fabricate the increasingly miniature devices that are literally changing our world. A bestseller in its first edition, Fundamentals of Microfabrication, Second Edition reflects the many developments in methods, materials, and applications that have emerged recently. Renowned author Marc Madou has added exercise sets to each chapter, thus answering the need for a textbook in this field. Fundamentals of Microfabrication, Second Edition offers unique, in-depth coverage of the science of miniaturization, its methods, and materials. From the fundamentals of lithography through bonding and packaging to quantum structures and molecular engineering, it provides the background, tools, and directions you need to confidently choose fabrication methods and materials for a particular miniaturization problem. New in the Second Edition Revised chapters that reflect the many recent advances in the field Updated and enhanced discussions of topics including DNA arrays, microfluidics, micromolding techniques, and nanotechnology In-depth coverage of bio-MEMs, RF-MEMs, high-temperature, and optical MEMs. Many more links to the Web Problem sets in each chapter
Now in its third edition, Fundamentals of Microfabrication and Nanotechnology continues to provide the most complete MEMS coverage available. Thoroughly revised and updated the new edition of this perennial bestseller has been expanded to three volumes, reflecting the substantial growth of this field. It includes a wealth of theoretical and practical information on nanotechnology and NEMS and offers background and comprehensive information on materials, processes, and manufacturing options. The first volume offers a rigorous theoretical treatment of micro- and nanosciences, and includes sections on solid-state physics, quantum mechanics, crystallography, and fluidics. The second volume presents a very large set of manufacturing techniques for micro- and nanofabrication and covers different forms of lithography, material removal processes, and additive technologies. The third volume focuses on manufacturing techniques and applications of Bio-MEMS and Bio-NEMS. Illustrated in color throughout, this seminal work is a cogent instructional text, providing classroom and self-learners with worked-out examples and end-of-chapter problems. The author characterizes and defines major research areas and illustrates them with examples pulled from the most recent literature and from his own work.