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Speckle study constitutes a multidisciplinary area with inherent complexities. In order to conquer challenges such as the variability of samples and sensitive measurements, researchers must develop a theoretical and statistical understanding of both biological and non-biological metrology using dynamic speckle laser. Dynamic Laser Speckle and Applications discusses the main methodologies used to analyze biospeckle phenomena with a strong focus on experimentation. After establishing a theoretical background in both speckle and biospeckle, the book presents the main methodologies for statistical and image analysis. It then deals with the concept of frequency decomposition before moving on to a discussion of fuzzy methods to treat dynamic speckle data. The book dedicates two sections to applications, including agricultural approaches. Additional features include photo images of experiments and software to aid in easy start-up of dynamic speckle usage. A systematic approach to new dynamic speckle laser phenomena, this book provides the physical theory and statistical background needed to analyze images formed by laser illumination in biological and non-biological samples.
This fourth volume of Light Scattering Reviews is composed of three parts. The ?rstpartisconcernedwiththeoreticalandexperimentalstudiesofsinglelightsc- tering by small nonspherical particles. Light scattering by small particles such as, for instance, droplets in the terrestrial clouds is a well understood area of physical optics. On the other hand, exact theoretical calculations of light scattering p- terns for most of nonspherical and irregularly shaped particles can be performed only for the restricted values of the size parameter, which is proportional to the ratio of the characteristic size of the particle to the wavelength?. For the large nonspherical particles, approximations are used (e. g. , ray optics). The exact th- retical techniques such as the T-matrix method cannot be used for extremely large particles, such as those in ice clouds, because then the size parameter in the v- iblex=2?a/???,wherea is the characteristic size (radius for spheres), and the associated numerical codes become unstable and produce wrong answers. Yet another problem is due to the fact that particles in many turbid media (e. g. , dust clouds) cannot be characterized by a single shape. Often, refractive indices also vary. Because of problems with theoretical calculations, experimental (i. e. , la- ratory) investigations are important for the characterization and understanding of the optical properties of such types of particles. The ?rst paper in this volume, written by B. Gustafson, is aimed at the descr- tionofscaledanalogueexperimentsinelectromagneticscattering.
This book examines Dynamic Light Scattering (DLS) and its derivatives Laser Doppler Flowmetry (LDF), Diffusing Wave Spectroscopy (DWS), Laser Speckle Contrast Imaging (LSCI), and Doppler Optical Coherence Tomography (OCT) for characterizing particle motion in turbid mediums like suspensions and solutions. It focuses on non-invasive blood flow imaging in biological tissues, detailing technological advancements, practical applications, and inherent challenges. Essential for professionals in biomedical optics and medical fields, as well as physics and engineering students, the book highlights its use in brain, skin, and micro-circulation studies, providing key insights and practical guidance. Key Features: • Presents a deep dive into DLS and its derivative techniques. • Emphasizes practical applications, including brain blood flow monitoring, skin perfusion measurements, and micro-circulation characterization. • Delivers insights into the challenges and limitations associated with DLS-based blood flow imaging.
This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Recent technological advances are significantly enhancing ones ability to image the interplay of neuronal activity, metabolism, and the associated vascular response with high spatial and temporal resolution. This Research Topic will cover these recent technological advances as well as the impact they have had on understanding the coupling of neuronal, metabolic, and vascular responses. We invite contributions to highlight new original research and to provide a forum for discussion of hot neurovascular topics. Potential contributions include, but are not limited by the following examples: - Development and application of novel optical technologies for imaging of neuronal, metabolic and vascular activity. Examples include 2-Photon Microscopy, Optical Coherence Tomography, and Second Harmonic Microscopy. - Intravital imaging of metabolites such as NADH and flavoproteins - Application of optical methods for manipulation of neuroglial circuits and vascular architectonics - Development of novel Magnetic Resonance contrasts for noninvasive imaging of blood flow, volume, and oxygen consumption. - Application of imaging tools for studying of neurovascular dysfunctions such as stroke, vascular dementia and Alzheimer’s Disease - Hypotheses, Perspectives, Commentaries and Opinions with regards to the body of recent publications that utilize imaging tools for investigation of neurogliovascular communication and the regulation of cerebral blood flow
These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving animals Imaging based on genetically encoded probes Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping Using pH-sensitive dyes for functional mapping Modulated imaging Calcium imaging of neuronal activity using 2-photon microscopy Fourier approach to optical imaging Fully updated chapters from the first edition Leading Authorities Explore the Latest Techniques Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.
Speckle study constitutes a multidisciplinary area with inherent complexities. In order to conquer challenges such as the variability of samples and sensitive measurements, researchers must develop a theoretical and statistical understanding of both biological and non-biological metrology using dynamic speckle laser. Dynamic Laser Speckle and Applications discusses the main methodologies used to analyze biospeckle phenomena with a strong focus on experimentation. After establishing a theoretical background in both speckle and biospeckle, the book presents the main methodologies for statistical and image analysis. It then deals with the concept of frequency decomposition before moving on to a discussion of fuzzy methods to treat dynamic speckle data. The book dedicates two sections to applications, including agricultural approaches. Additional features include photo images of experiments and software to aid in easy start-up of dynamic speckle usage. A systematic approach to new dynamic speckle laser phenomena, this book provides the physical theory and statistical background needed to analyze images formed by laser illumination in biological and non-biological samples.
Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, Cerebral Angiogenesis: Methods and Protocols aims to define the cellular and molecular mechanisms underlying this important process. Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety of different models in which cerebral angiogenesis can be studied, methods to characterize and quantify angiogenic events as well as several different approaches to measure changes in cerebral blood flow, different approaches to investigate the role of specific candidate genes in cerebral angiogenesis, methods of therapeutically manipulating cerebral angiogenesis by gene delivery, and finally important approaches to examine cerebral angiogenic mechanisms in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and decidedly practical, Cerebral Angiogenesis: Methods and Protocols should be a vital resource for all researchers, both those new to this field as well as those looking to use more specialized and sophisticated techniques to examine blood vessel growth in the brain.
The Biomed 2011 brought together academicians and practitioners in engineering and medicine in this ever progressing field. This volume presents the proceedings of this international conference which was hold in conjunction with the 8th Asian Pacific Conference on Medical and Biological Engineering (APCMBE 2011) on the 20th to the 23rd of June 2011 at Berjaya Times Square Hotel, Kuala Lumpur. The topics covered in the conference proceedings include: Artificial organs, bioengineering education, bionanotechnology, biosignal processing, bioinformatics, biomaterials, biomechanics, biomedical imaging, biomedical instrumentation, BioMEMS, clinical engineering, prosthetics.