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A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. - Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions - Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT - Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence - Includes quality control information crucial in research and clinical laboratories - Most chapters are written by the inventors and principal developers of the methodologies - Includes color plates, 77 figures, and 18 tables
The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.
A concise clinical reference that facilitates the diagnosis of intrauterine and perinatally acquired infections was the goal in creating the Congenital and Perinatal Infections: A Concise Guide to Diagnosis. Information about the natural history, m- agement, and outcome of these infections is well detailed in many other sources and so has not been included. Rather, the focus of the book is diagnosis. The initial chapters provide general information about serological and nonserological assays that are used for the diagnosis of infections, and a chapter about the placenta includes details about histopathological findings that can be helpful with the diagnosis of congenital inf- tions. The remainder of the book is devoted to the diagnosis of specific congenital and/ or perinatal infections. As illustrated in the chapters about specific infections, the approach to diagnosis of a congenital or perinatally acquired infection in the neonate begins, when possible, with consideration and diagnosis of infection in the pregnant woman, knowledge of how the infection is transmitted, and the risk of that infection for the woman and her fetus or neonate. The possibility of congenital or perinatal infection in neonates is usually considered because of the diagnosis of, or concern about a s- cific infection in, a mother during pregnancy that can be transmitted to the neonate or because of clinical findings in the neonate at birth that suggest an infectious cause.
This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.
Shanghai COVID-19 Medical Treatment Expert Team edits this timely guide for effective prevention and control of COVID-19. Readers will obtain useful guidance on prevention and control of COVID-19 in different places ranging from homes, outdoors, workplaces, etc. You will know 'What is the purpose and significance of home quarantine?', ' When do you need to wear a mask?', 'How should you wash your hands?', 'Do you need to wear a mask in an elevator?', 'What foods are safe to eat and what are not?', 'How to deal with express parcels from major epidemic areas or other areas?' and many other useful tips.Related Link(s)
The essential reference of clinical virology Virology is one of the most dynamic and rapidly changing fields of clinical medicine. For example, sequencing techniques from human specimens have identified numerous new members of several virus families, including new polyomaviruses, orthomyxoviruses, and bunyaviruses. Clinical Virology, Fourth Edition, has been extensively revised and updated to incorporate the latest developments and relevant research. Chapters written by internationally recognized experts cover novel viruses, pathogenesis, epidemiology, diagnosis, treatment, and prevention, organized into two major sections: Section 1 provides information regarding broad topics in virology, including immune responses, vaccinology, laboratory diagnosis, principles of antiviral therapy, and detailed considerations of important organ system manifestations and syndromes caused by viral infections. Section 2 provides overviews of specific etiologic agents and discusses their biology, epidemiology, pathogenesis of disease causation, clinical manifestations, laboratory diagnosis, and management. Clinical Virology provides the critical information scientists and health care professionals require about all aspects of this rapidly evolving field.
This volume covers multidisciplinary approaches on various aspects of Chikungunya Virus (CHIKV) research that was brought together from leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, bioinformatics, and proteomics approaches in cellular response studies. Chikungunya Virus: Methods and Protocols also discusses methods in immunology and animal model studies, as well as different strategies of antivirals and vaccine development for therapeutics against CHIKV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Chikungunya Virus: Methods and Protocols is a useful and comprehensive resource for CHIKV researchers and anyone interested in learning more about the Chikungunya Virus.
Biosafety in the Laboratory is a concise set of practical guidelines for handling and disposing of biohazardous material. The consensus of top experts in laboratory safety, this volume provides the information needed for immediate improvement of safety practices. It discusses high- and low-risk biological agents (including the highest-risk materials handled in labs today), presents the "seven basic rules of biosafety," addresses special issues such as the shipping of dangerous materials, covers waste disposal in detail, offers a checklist for administering laboratory safetyâ€"and more.
Detection and Analysis of SARS Coronavirus Detecting and analyzing the COVID-19 pandemic with biosensor technology The highly contagious SARS CoV-2 pathogen has challenged health systems around the world as they struggle to detect and monitor the spread of the pathogen. In Detection and Analysis of SARS Coronavirus: Advanced Biosensors for Pandemic Viruses and Related Pathogens expert chemists Chaudhery Mustansar Hussain and Sudheesh K. Shukla deliver a practical analysis of how contactless coronavirus detectors may be developed using existing biosensor technology. The editors outline current challenges in the field, the bioanalytical principles for coronavirus detection, and available biosensor technology. They then move on to how available technology might be adapted to detect coronaviruses and how commercialization of the technology might unfold. The lessons learned in this book are readily applicable to the study of other current and emerging pathogens. Readers will also enjoy: A thorough introduction to the current diagnostic approaches for COVID-19, including common challenges, technology adaptation, and future potential An exploration of bio-analytical strategies for SARS CoV-2/COVID-19, including COVID detection via nanotechnology, biosensing approaches, and the role of nanotechnology in coronavirus detection Practical discussions of biosensors for the analysis of SARS CoV-2/COVID-19, including sensor development for coronavirus and chemical sensors for coronavirus diagnosis In-depth treatments of the commercialization and standardization for analytical technologies Perfect for virologists, pharmaceutical industry professionals, and sensor developers, Detection and Analysis of SARS Coronavirus is also an indispensable resource for those working in analytical research institutes, biotechnology industry professionals, and public health agency professionals.