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Plant bacterial diseases are a major factor in economic crop losses worldwide.The transportation and use of clean, pathogen-free seed and propagative material is the most vital step in controlling bacterial diseases, but this requires accurate, rapid, sensitive, and reliable pathogen detection.The all-new second edition of Detection of Plant-Pathogenic Bacteria in Seed and Other Planting Material is an essential, easy-to-use lab manual describing and illustrating the latest methods for detecting a wide range of bacteria responsible for many of the world’s most economically significant bacterial plant diseases in crops.Yet this book does more than describe and illustrate the use of real-time PCR, rapid PCR-based assays, and other methods for specific, sensitive detection of bacteria in seed and propagative material. Its 48 chapters offer background information on the given bacteria and the diseases they cause, describe symptoms, detail their epidemiology, identify strategies for disease management, and provide protocols for detection in thorough detail.
The need for detection assays. Review of extraction and isolation methods. Detection and identification of bacteria. Detection of plant paghogenic bacteria in grain legumes and cereals. Detection of plant pathogenic bacteria in vegetables. Detection of plant pathogenic bacteria in vegetatively propagated crops.
Identification schemes; Gram-negative bacteria; Gram-positive bacteria; Cell wall-free prokaryotes.
Significant advancements have been made in pathogen detection technologies during the last decade. Indexing of plants and plant parts for the presence of specific pathogens has been most effective in some instances for avoiding and/or controlling disease. The new technologies for detecting low levels of pathogens will increase the value of indexing as a tool for plant disease control. Providing an overview of the status of detection technology, this volume is directed not only to scientists and students interested in detection technology, but also to those interested in formulating and implementing disease control and quarantine regulations. This book provides a conceptual framework which presents the current scientific literature, state-of-the-art assessments, and speculations on future developments and requirements of pathogen indexing methods. Chapters cover the different pathogen groups, review current practices in areas where detection technology has become important, and provide perspectives on how indexing technologies can be applied, how well it has worked, and which problems remain. Statistical treatment of detection limits, sampling strategies, risk assessment, cost, standardization, and quality control are also covered.
Rice seed health and quarantine; The rice plant and its environment; Equipment; Samples and sampling; dry seed inspection; Fungi; Bacteria; Nematodes; Viruses and mycoplasmalike organisms; Field inspection; Seed treatment; Weed seed; Insect pests; Fungal pathogens; Bacterial pathogens; Nematode pest; Organisms causing grain discoloration and damage.
This substantially updated edition now in full colour provides key techniques used when working with fungal and fungal-like plant pathogens. As a practical manual it also deals with disease recognition, detection and identification of fungi, plus methods to characterise and curate fungi and handle them under quarantine and quality assurance systems. Fungal Plant Pathogens: Applied Techniques, 2nd edition provides a valuable guide to investigating fungal plant diseases and interpreting laboratory findings for postgraduate and advanced undergraduate students, extension plant pathologists, consultants and advisers in agriculture, forestry and horticulture, and the food supply chain.
The global population is increasing rapidly, and feeding the ever-increasing population poses a serious challenge for agriculturalists around the world. Seed is a basic and critical input in agriculture to ensure global food security. Roughly 90 percent of the crops grown all over the world are propagated by seed. However, seed can also harbour and spread pathogens, e.g. fungi, bacteria, nematodes, viruses etc., which cause devastating diseases. Seed-borne pathogens represent a major threat to crop establishment and yield. Hence, timely detection and diagnosis is a prerequisite for their effective management. The book "Seed-Borne Diseases of Agricultural Crops: Detection, Diagnosis & Management" addresses key issues related to seed-borne/transmitted diseases in various agricultural crops. Divided into 30 chapters, it offers a comprehensive compilation of papers concerning: the history of seed pathology, importance of seed-borne diseases, seed-borne diseases and quarantine, seed health testing and certification, detection and diagnosis of seed-borne diseases and their phytopathogens, host-parasite interactions during development of seed-borne diseases, diversity of seed-borne pathogens, seed-borne diseases in major agricultural crops, non-parasitic seed disorders, mechanisms of seed transmission and seed infection, storage fungi and mycotoxins, impact of seed-borne diseases on human and animal health, and management options for seed-borne diseases. We wish to thank all of the eminent researchers who contributed valuable chapters to our book, which will be immensely useful for students, researchers, academics, and all those involved in various agro-industries.
Provides fundamental knowledge every plant scientist and student of plant pathology should know, including important historical events that gave birth to the field as well as its recent advances. Illustrates the symptoms caused by bacteria in a way that facilitates comprehension of the many different types of plant diseases that they cause. Each symptom type is presented with a detailed example of a causal agent and its characteristics, diagnostics, and mechanisms of virulence and pathogenicity. Also includes an extended discussion on the molecular mechanisms of virulence and a chapter on epidemiology and disease control.
This volume continues the series of books on “Plant Pathology in the 21st Century”, and contains the papers given at the 10th International Congress of Plant Pathology (ICPP 2013) held in Beijing, August 25-30, 2013 concerning seed health. Many pathogens are transmitted throughout infected seeds and propagation material .The fact that propagation material production is very much concentrated in few establishments, favors the quick spread of new diseases throughout seed commercialization. This phenomenon is very much accelerated in a globalized system. The book covers case studies of contamination, aspects of detection and diagnosis as well as disease management strategies, with special emphasis towards seed treatments with unconventional products. This book will be useful for all plant pathologists as well as students in advanced courses.