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Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.
This book discusses recent trends and developments in the microbial conversion process, which serves as an important route for biofuel production, with particular attention to bioreactors. It combines microbial conversion with multiphase flow and mass transfer, providing an alternative perspective for the understanding of microbial biomass and energy production process as well as enhancement strategy. This book is relevant to students and researchers who work in the fields of renewable energy, engineering and biotechnology. Policymakers, economists and industry engineers also benefit from this book, as it can be used as a resource for the implementation of renewable energy technologies.
This book critically discusses different aspects of algal production systems and several of the drawbacks related to microalgal biomass production, namely, low biomass yield, and energy-consuming harvesting, dewatering, drying and extraction processes. These provide a background to the state-of-the-art technologies for algal cultivation, CO2 sequestration, and large-scale application of these systems. In order to tap the commercial potential of algae, a biorefinery concept has been proposed that could help to extract maximum benefits from algal biomass. This refinery concept promotes the harvesting of multiple products from the feedstock so as to make the process economically attractive. For the last few decades, algal biomass has been explored for use in various products such as fuel, agricultural crops, pigments and pharmaceuticals, as well as in bioremediation. To meet the huge demand, there has been a focus on large-scale production of algal biomass in closed or open photobioreactors. Different nutritional conditions for algal growth have been explored, such as photoautotrophic, heterotrophic, mixotrophic and oleaginous. This book is aimed at a wide audience, including undergraduates, postgraduates, academics, energy researchers, scientists in industry, energy specialists, policy makers and others who wish to understand algal biorefineries and also keep abreast of the latest developments.
Bioremediation refers to the clean‐up of pollution in soil, groundwater, surface water, and air using typically microbiological processes. It uses naturally occurring bacteria and fungi or plants to degrade, transform or detoxify hazardous substances to human health or the environment. For bioremediation to be effective, microorganisms must enzymatically attack the pollutants and convert them to harmless products. As bioremediation can be effective only where environmental conditions permit microbial growth and action, its application often involves the management of ecological factors to allow microbial growth and degradation to continue at a faster rate. Like other technologies, bioremediation has its limitations. Some contaminants, such as chlorinated organic or high aromatic hydrocarbons, are resistant to microbial attack. They are degraded either gradually or not at all, hence, it is not easy to envisage the rates of clean-up for bioremediation implementation. Bioremediation represents a field of great expansion due to the important development of new technologies. Among them, several decades on metagenomics expansion has led to the detection of autochthonous microbiota that plays a key role during transformation. Transcriptomic guides us to know the expression of key genes and proteomics allow the characterization of proteins that conduct specific reactions. In this book we show specific technologies applied in bioremediation of main interest for research in the field, with special attention on fungi, which have been poorly studied microorganisms. Finally, new approaches in the field, such as CRISPR-CAS9, are also discussed. Lastly, it introduces management strategies, such as bioremediation application for managing affected environment and bioremediation approaches. Examples of successful bioremediation applications are illustrated in radionuclide entrapment and retardation, soil stabilization and remediation of polycyclic aromatic hydrocarbons, phenols, plastics or fluorinated compounds. Other emerging bioremediation methods include electro bioremediation, microbe-availed phytoremediation, genetic recombinant technologies in enhancing plants in accumulation of inorganic metals, and metalloids as well as degradation of organic pollutants, protein-metabolic engineering to increase bioremediation efficiency, including nanotechnology applications are also discussed.
This book addresses microalgae, which represent a very promising biomass resource for wastewater treatment and producing biofuels. Accordingly, microalgae are also an expanding sector in biofuels and wastewater treatment, as can be seen in several high-profile start-ups from around the globe, including Solix Biofuels, Craig Venter’s Synthetic Genomics, PetroSun, Chevron Corporation, ENN Group etc. In addition, a number of recent studies and patent applications have confirmed the value of modern microalgae for biofuels production and wastewater treatment systems. However, substantial inconsistencies have been observed in terms of system boundaries, scope, the cultivation of microalgae and oil extraction systems, production costs and economic viability, cost-lowering components, etc. Moreover, the downstream technologies and core principles involved in liquid fuel extraction from microalgae cells are still in their early stages, and not always adequate for industrial production. Accordingly, multilateral co-operation between universities, research institutes, governments, stakeholders and researchers is called for in order to make microalgae biofuels economical. Responding to this challenge, the book begins with a general introduction to microalgae and the algae industry, and subsequently discusses all major aspects of microalgal biotechnology, from strain isolation and robust strain development, to biofuel development, refinement and wastewater treatment.
This book focuses on various types of bioactive compounds, including secondary metabolites, oligosaccharides, polysaccharides, flavonoids, peptides/proteins, carotenoid pigments, quinones, terpenes, and polyunsaturated fatty acids, and presents an overview of their nutraceutical activities. It covers the current status and future potential of food compounds, as well as extraction technologies for bioactives derived from plant, fungi and marine-derived bioactive agents. Finally, health-promoting effects of plant, fungi and marine-derived bioactive agents are discussed. Chapters come from top researchers in this area from around the globe. The volume caters to the needs of undergraduate and post-graduate students in the area of food biotechnology, food bioprocessing, biotechnology, food engineering, etc., and also contains information pertinent to researchers.
This book presents the select peer-reviewed proceedings of the International Conference on Advances in Bioprocess Engineering and Technology (ICABET 2020). The book covers all aspects of bioprocesses, especially related to fermentation technology, food technology, environmental biotechnology, and sustainable energy. Along with this primary theme, the focus is on recent advances in bioprocessing research such as biosensors, micro-reactors, novel separation techniques, bioprocess control, bio-safety, advanced techniques for waste to wealth generation, and nanobiotechnology. This contents are divided according to the major themes of the conference: (i) Fermentation Technology and Bioreactor, (ii) Food Pharmaceuticals and Health care, (iii) Environment and Agriculture, and (iv) Sustainable Energy. This book is intended to help students, researchers, and industry professionals acquire knowledge on innovative technologies and recent advancements in the field of bioprocess engineering and technology.
"Microalgae Biotechnology for Food, Health and High Value Products” presents the latest technological innovations in microalgae production, market status of algal biomass-based products, and future prospects for microalgal applications. It provides stimulating overviews from different perspectives of application that demonstrate how rapidly the commercial production of microalgae-based food, health and high value products is advancing. It also addresses a range of open questions and challenges in this field. The book highlights the latest advances of interest to those already working in the field, while providing a comprehensive overview for those readers just beginning to learn about the promise of microalgae as a sustainable source of both specialty and commercial products. It offers a valuable asset for commercial algae producers, algae product developers, scientific researchers and students who are dedicated to the advancement of microalgae biotechnology for applications in health, diet, nutrition, cosmetics, biomaterials etc.
This book presents a broad range of technologies for sustainable agrochemistry, e.g. semiochemicals for pest management, nanotechnology for release of eco-friendly agrochemicals, and green chemistry principles for agriculture. It provides a concise introduction to sustainable agrochemistry for a professional audience, and highlights the main scientific and technological approaches that can be applied to modern agrochemistry. It also discusses various available technologies for reducing the negative impacts of agrochemicals on the environment and human health.
This book reviews the production of bioplastic from various raw materials and recycling wastewater into useful bioproducts by bacteria. In addition, it also addresses the recent advancement in pest control in rice plants, different methods to analyse genotoxicity on soil samples and the effect of phytocompounds on acrylamide-induced toxicity in Drosophilla. Interestingly, this book also discusses mesoporous silica nanoparticles' role as nanocarrier material for inhibiting the cancer cell, especially breast cancer and various biotechnological applications of marine fungal exopolysaccharides.