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Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. - Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research - Offers insight into common challenges in implementing techniques, as well as effective solutions
Learn about the theory, techniques and applications of wavefront shaping in biomedical imaging using this unique text. With authoritative contributions from researchers who are defining the field, cutting-edge theory is combined with real-world practical examples, experimental data and the latest research trends to provide the first book-level treatment of the subject. It is suitable for both background reading and use in a course, with coverage of essential topics such as adaptive optical microscopy, deep tissue microscopy, time reversal and optical phase conjugation, and tomography. The latest images from the forefront of biomedical imaging are included, and full-colour versions are available in the eBook version. Researchers, practitioners and graduate students in optics, biophotonics, biomedical engineering, and biology who use biomedical imaging tools and are looking to advance their knowledge of the subject will find this an indispensable resource.
Biomedical optics holds tremendous promise to deliver effective, safe, non- or minimally invasive diagnostics and targeted, customizable therapeutics. Handbook of Biomedical Optics provides an in-depth treatment of the field, including coverage of applications for biomedical research, diagnosis, and therapy. It introduces the theory and fundamental
"Alberto Diaspro has been choreographing light's dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology's secrets."- From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimens
Adaptive Optics for Biological Imaging brings together groundbreaking research on the use of adaptive optics for biological imaging. The book builds on prior work in astronomy and vision science. Featuring contributions by leaders in this emerging field, it takes an interdisciplinary approach that makes the subject accessible to nonspecialists who want to use adaptive optics techniques in their own work in biology and bioengineering. Organized into three parts, the book covers principles, methods, and applications of adaptive optics for biological imaging, providing the reader with the following benefits: Gives a general overview of applied optics, including definitions and vocabulary, to lay a foundation for clearer communication across disciplines Explains what kinds of optical aberrations arise in imaging through various biological tissues, and what technology can be used to correct for these aberrations Explores research done with a variety of biological samples and imaging instruments, including wide-field, confocal, and two-photon microscopes Discusses both indirect wavefront sensing, which uses an iterative approach, and direct wavefront sensing, which uses a parallel approach Since the sample is an integral part of the optical system in biological imaging, the field will benefit from participation by biologists and biomedical researchers with expertise in applied optics. This book helps lower the barriers to entry for these researchers. It also guides readers in selecting the approach that works best for their own applications.
This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
"This beautiful book can be read as a novel presenting carefully our quest to get more and more information from our observations and measurements. Its authors are particularly good at relating it." --Pierre C. Sabatier "This is a unique text - a labor of love pulling together for the first time the remarkably large array of mathematical and statistical techniques used for analysis of resolution in many systems of importance today – optical, acoustical, radar, etc.... I believe it will find widespread use and value." --Dr. Robert G.W. Brown, Chief Executive Officer, American Institute of Physics "The mix of physics and mathematics is a unique feature of this book which can be basic not only for PhD students but also for researchers in the area of computational imaging." --Mario Bertero, Professor, University of Geneva "a tour-de-force covering aspects of history, mathematical theory and practical applications. The authors provide a penetrating insight into the often confused topic of resolution and in doing offer a unifying approach to the subject that is applicable not only to traditional optical systems but also modern day, computer-based systems such as radar and RF communications." --Prof. Ian Proudler, Loughborough University "a ‘must have’ for anyone interested in imaging and the spatial resolution of images. This book provides detailed and very readable account of resolution in imaging and organizes the recent history of the subject in excellent fashion.... I strongly recommend it." --Michael A. Fiddy, Professor, University of North Carolina at Charlotte This book brings together the concept of resolution, which limits what we can determine about our physical world, with the theory of linear inverse problems, emphasizing practical applications. The book focuses on methods for solving illposed problems that do not have unique stable solutions. After introducing basic concepts, the contents address problems with "continuous" data in detail before turning to cases of discrete data sets. As one of the unifying principles of the text, the authors explain how non-uniqueness is a feature of measurement problems in science where precision and resolution is essentially always limited by some kind of noise.