Download Free Cloning Book in PDF and EPUB Free Download. You can read online Cloning and write the review.

Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species—including mice, sheep, cattle, and non-mammals—is considered as well. The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine – Dr John Gurdon; the cloning of the first mammal from a somatic cell – Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual – Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell – Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas. First and most comprehensive book on animal cloning, 100% revised Describes an in-depth analysis of current limitations of the technology and research areas to explore Offers cloning applications on basic biology, agriculture, biotechnology, and medicine
Human reproductive cloning is an assisted reproductive technology that would be carried out with the goal of creating a newborn genetically identical to another human being. It is currently the subject of much debate around the world, involving a variety of ethical, religious, societal, scientific, and medical issues. Scientific and Medical Aspects of Human Reproductive Cloning considers the scientific and medical sides of this issue, plus ethical issues that pertain to human-subjects research. Based on experience with reproductive cloning in animals, the report concludes that human reproductive cloning would be dangerous for the woman, fetus, and newborn, and is likely to fail. The study panel did not address the issue of whether human reproductive cloning, even if it were found to be medically safe, would beâ€"or would not beâ€"acceptable to individuals or society.
Animal cloning has developed quickly since the birth of Dolly the sheep. Yet many of the first questions to be raised still need to be answered. What do Dolly and her fellow mouse, cow, pig, goat and monkey clones mean for science? And for society? Why do so many people respond so fearfully to cloning? What are the ethical issues raised by cloning animals, and in the future, humans? How are the makers of public policy coping with the stunning fact that an entire animal can be reconstructed from a single adult cell? And that humans might well be next? The Cloning Source Book addresses all of these questions in a way that is unique in the cloning literature, by grounding what is effectively an interdisciplinary conversation in solid science. In the first section of the book, the key scientists responsible for the early and crucial developments in cloning speak to us directly, and other scientists evaluate and comment on these developments. The second section explores the context of cloning and includes sociological, mythological, and historical perspectives on science, ethics, and policy. The authors also examine the media's treatment of the Dolly story and its aftermath, both in the United States and in Britain. The third section, on ethics, contains a broad range of papers written by some of the major commentators in the field. The fourth section addresses legal and policy issues. It features individual and collective contributions by those who have actually shaped public policy on reproductive cloning, therapeutic cloning, and similarly contentious bioethical issues in the United States, Britain, and the European Union. Animal cloning continues for agricultural and medicinal purposes, the latter in combination with transgenics. Human cloning for therapeutic purposes has recently been made legal in Britain. The goal is to produce an early embryo and then derive stem cells that are immunologically matched to the donor. Two human reproductive cloning projects have been announced, and there are almost certainly others about which we know nothing. Sooner or later a cloned human will be born. Many lessons can be learned from the cloning experience. Most importantly, there needs to be a public conversation about the permissible uses of new and morally murky technologies. Scientists, journalists, ethicists and policy makers all have roles to play, but cutting-edge science is everybody's business. The Cloning Sourcebook provides the tools required for us to participate in shaping our own futures.
Human cloning raises the most profound questions about human nature, our faith in ourselves, and our ability to make decisions that could significantly alter the character of humanity. In this exciting and accessible book, Gregory Pence offers a candid and sometimes humorous look at the arguments for and against human cloning. Originating a human being by cloning, Pence boldly argues, should not strike fear in our hearts but should be examined as a reasonable reproductive option for couples. Pence considers how popular culture has influenced the way we think about cloning, and he presents a lucid and non-technical examination of the scientific research and relevant moral issues in the cloning debate. This book is a must-read for anyone who is concerned about the impact of technology on human life and for those with interests in medical ethics, sociology, and public policy.
This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.
Long before scientists at the Roslin Institute in Scotland cloned Dolly the sheep in 1996, American embryologist and aspiring cancer researcher Robert Briggs successfully developed the technique of nuclear transplantation using frogs in 1952. Although the history of cloning is often associated with contemporary ethical controversies, Forgotten Clones revisits the influential work of scientists like Briggs, Thomas King, and Marie DiBerardino, before the possibility of human cloning and its ethical implications first registered as a concern in public consciousness, and when many thought the very idea of cloning was experimentally impossible. By focusing instead on new laboratory techniques and practices and their place in Anglo-American science and society in the mid-twentieth century, Nathan Crowe demonstrates how embryos constructed in the lab were only later reconstructed as ethical problems in the 1960s and 1970s with the emergence of what was then referred to as the Biological Revolution. His book illuminates the importance of the early history of cloning for the biosciences and their institutional, disciplinary, and intellectual contexts, as well as providing new insights into the changing cultural perceptions of the biological sciences after Second World War.
Examines the history, current developments, future, and ethical ramifications of cloning, recombinant DNA, and gene therapy.
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
In Human Cloning a panel of distinguished philosophers, medical ethicists, religious thinkers, and social critics tackle the thorny problems raised by the now real possibility of human cloning. In their wide ranging reviews, the distinguished contributors critically examine the major arguments for and against human cloning, probe the implications of such a procedure for society, and critically evaluate the "Report and Recommendations of the National Bioethics Advisory Commission." The debate includes both religious and secular arguments, as well as an outline of the history of the cloning debate and a discussion of human cloning's impact on our sense of self and our beliefs about the meaning of life.
This collection of papers details the prospective medical benefits for development of pharmaceuticals in transgenic animals and of organs for xenotransplants, and the implications for human cloning.