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Chromatin and Chromosomal Protein Research II
Chromatin and Chromosomal Protein Research I
Isolation of nuclei and preparation of chromatin. II. Procedures for minimizing preotease activity during isolation of nuclei, chromatin, and the histones. Isolation of nuclei from animal cells in culture. Preparation of chromatin from animal tissues and cultured cells. Methods for isolation of nuclei from cultured mammalian cells; conditions for preferential retention of selected histones. Methods for isolation of nuclei from spermatozoa. Manual enucleation of Xenopus oocytes. Macro and micro methods for isolation and infection of cell components from polytene tissues. Isolation of nuclei and chromatin from phycomyces blakesleeanus. Chromosomes. Mammalian metaphase chromosomes. Analytical techniques for isolated metaphase chromosome fractions. Methods and mechanisms of chromosome banding. Cytological analysis of drosophila polytene chromosomes. Isolation and purification of nucleoli and nucleolar chromatin from mammalian cells. Nucleolar proteins. Fractionation and characterization of histones. II...
METHODS IN CELL BIOLOGY,VOLUME 19: CHROMATIN AND CHROMOSOMAL PROTEIN RESEARCH IV.
Chromatin and Chromosomal Protein Research IV
Chromatin and Chromosomal Protein Research III
Chromatin and Chromosome Structure consists of chapters that reflect a 1975 seminar course and the reported observations after the session. The seminar is organized for the benefit of both faculty and students in the Biology Ph.D. Program of the City University of New York. This book begins with a presentation of the conformational studies of histones. It then describes the histone-DNA interactions and the subunits isolated either from chromatin or from nuclei. It also explains the post-synthetic modifications of histone structure. Furthermore, the book talks about the chemistry and function of nuclear nonhistone proteins, low molecular weight nuclear RNA, and polytene chromosome structure. The regulation of gene expression in chick oviduct model system and the main features of lampbrush chromosomes are shown as well. Aside from the students and faculty of the above-mentioned university, this compilation will be invaluable as well to other undergraduate and graduate students; professors; and researchers. It will also be a good reference to those who are specializing in chromatin and chromosome studies.