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Biodegradation plays an important role in the removal of hydrocarbons from the environment. Compared to the extensive studies concerning aerobic hydrocarbon degradation, there is still a lack of knowledge with regard to the degradation of hydrocarbons under anoxic conditions. The current thesis, containing three subprojects, focused on the anaerobic degradation of the BTEX (abbreviation for benzene, toluene, ethylbenzene, xylene) compound - especially ethylbenzene, n-alkanes and phenols. Phenols are close relatives of BTEX compounds; the biodegradation pathways of phenols and BTEX share many similarities with regard to the activation mechanisms and the enzymes involved. Stable isotope analysis was applied in this thesis as the main tool for the characterization of hydrocarbon degradation pathways and involved microorganisms. The carbon or hydrogen stable isotope fractionation patterns upon biodegradation of TEX, n-alkanes and phenols were determined to elucidate the degradation pathways. Carbon stable isotope labelling method was applied to identify the key-players in an ethylbenzene-degrading enrichment culture. ...
In this book international experts discuss the state-of-the-art in the biological degradation of hydrocarbons to meet remedial or disposal goals. The work focuses on practical applications, often on globally important scales including the remediation of some of the world’s largest crude oil spills. Other related chapters discuss important implications of microbial transformation of hydrocarbons, including treatment of high fat processing wastes, impacts of microbial biodegradation activity on industrial processes, and the implications of microbial oil degradation in relation to modern oil extraction processes like hydraulic fracturing of shales and extraction of oil sands.
The use of Compound-specific Stable Isotope Analysis (CSIA) is increasing in many areas of science and technology for source allocation, authentication, and characterization of transformation reactions. Until now, there have been no textbooks available for students with an analytical chemical background or basic introductory books emphasising the instrumentation and theory. This book is the first to focus solely on stable isotope analysis of individual compounds in sometimes complex mixtures. It acts as both a lecture companion for students and a consultant for advanced scientists in fields including forensic and environmental science. The book starts with a brief history of the field before going on to explain stable isotopes from scratch. The different ways to express isotope abundances are introduced together with isotope effects and isotopic fractionation. A detailed account of the required technical equipment and general procedures for CSIA is provided. This includes sections on derivatization and the use of microextraction techniques in GC-IRMS. The very important topic of referencing and calibration in CSIA is clearly described. This differs from approaches used in quantitative analysis and is often difficult for the newcomer to comprehend. Examples of successful applications of CSIA in food authenticity, forensics, archaeology, doping control, environmental science, and extraterrestrial materials are included. Applications in isotope data treatment and presentation are also discussed and emphasis is placed on the general conclusions that can be drawn from the uses of CSIA. Further instrumental developments in the field are highlighted and selected experiments are introduced that may act as a basis for a short practical course at graduate level.
Enhanced analytical capabilities and separation techniques, improved detection limits, and accessibility of instrumentation have led to massive strides in the use of isotopes to assess microbial processes in surface and subsurface sediments. Considering the rapid growth of research and commercial interest in stable isotope and radioisotope applicat
Proceedings of the 42nd OHOLO Conference held in Eilat, Israel, May 3-7, 1998
Bioavailability refers to the extent to which humans and ecological receptors are exposed to contaminants in soil or sediment. The concept of bioavailability has recently piqued the interest of the hazardous waste industry as an important consideration in deciding how much waste to clean up. The rationale is that if contaminants in soil and sediment are not bioavailable, then more contaminant mass can be left in place without creating additional risk. A new NRC report notes that the potential for the consideration of bioavailability to influence decision-making is greatest where certain chemical, environmental, and regulatory factors align. The current use of bioavailability in risk assessment and hazardous waste cleanup regulations is demystified, and acceptable tools and models for bioavailability assessment are discussed and ranked according to seven criteria. Finally, the intimate link between bioavailability and bioremediation is explored. The report concludes with suggestions for moving bioavailability forward in the regulatory arena for both soil and sediment cleanup.
This handbook provides a comprehensive overview of microbial interactions with the major forms of hydrocarbons, oils, and lipids in or entering the biosphere. It is the definitive resource on the physiological mechanisms and adaptive strategies characteristic of the microbial lifestyle that plays out at hydrophobic material: aqueous liquid interfaces.
The book discusses ways to overcome the side effects of using hydrocarbon-based products as energy sources. Hydrocarbons produce raw crude oil waste of around 600,000 metric tons per annum, with a range of uncertainty of 200,000 metric tons per year. The various chapters in this book focus on approaches to reduce these wastes through the application of potential microbes, in a process called bioremediation. The book is a one-stop reference resource on the methods, mechanisms and application of the bio-composites, in the laboratory and field. Focusing on resolving a very pressing environmental issue, it not only provides details of existing challenges, but also offers deeper insights into the possibility of solving problems using hydrocarbon bioremediation.