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V. 1. Laboratory procedures and their applications.--v. 2. Cell growth, nutrition, cytodifferentiation, and cryopreservation.--v. 3. Plant regeneration and genetic variability.--v. 4. Cell culture in phytochemistry.--v. 5. Phytoche micals in plant cell cultures.--v. 6. Molecular biology of plant nuclear genes. --v. 7A. The molecular biology of plastids.--v. 7B. The photosynthetic apparatus: molecular biology and operation.--v. 8. Scale-up and automation in plant propagation.
V. 1. Laboratory procedures and their applications.--v. 2. Cell growth, nutrition, cytodifferentiation, and cryopreservation.--v. 3. Plant regeneration and genetic variability.--v. 4. Cell culture in phytochemistry.--v. 5. Phytoche micals in plant cell cultures.--v. 6. Molecular biology of plant nuclear genes. --v. 7A. The molecular biology of plastids.--v. 7B. The photosynthetic apparatus: molecular biology and operation.--v. 8. Scale-up and automation in plant propagation.
V. 1. Laboratory procedures and their applications.--v. 2. Cell growth, nutrition, cytodifferentiation, and cryopreservation.--v. 3. Plant regeneration and genetic variability.--v. 4. Cell culture in phytochemistry.--v. 5. Phytoche micals in plant cell cultures.--v. 6. Molecular biology of plant nuclear genes. --v. 7A. The molecular biology of plastids.--v. 7B. The photosynthetic apparatus: molecular biology and operation.--v. 8. Scale-up and automation in plant propagation.
Cell Growth, Nutrition, Cytodifferentiation, and Cryopreservation
Plant tissue culture is commonly used to describe the in-vitro and aseptic growth of any plant part on a nutrient medium. This technology is based on three fundamental objectives,1) The plant part or explants must be isolated from the rest of plant body,2) The explants must be maintained in controlled physically (environmental) and chemically designed (nutrient medium) conditions.3) Asepsis must be maintainedIt is required for asepsis to maintain a high degree of cleanliness in the laboratory, whether the techniques are being used for simple propagation, as a method to study genetic, metabolic or development charges in a model system, or for the creation of new plant variations via genetic engineering. There are a number of basic facilities and a minimum level of organization that should be available to the people working in the laboratory. The basic facility comprises of the following.
Plant Improvement and Somatic Cell Genetics includes all but one of the papers presented at two symposia held during the XIII International Botanical Congress in Sydney, Australia, on August 21-28, 1981. ""Frontiers in Plant Breeding"" and ""Cell Culture and Somatic Cell Genetics in Plant Biology"" highlight the ways in which plant breeding techniques can improve crops. The book explores the potentials as well as the limitations of plant breeding, and cellular and molecular techniques in plant improvement. Comprised of 14 chapters, this volume begins with an overview of the potential applications of exotic germplasm for tomato and cereal crop improvement. It continues with a discussion of multiline breeding, breeding of crop plants that can tolerate soil stresses, combining genomes by means of conventional methods, use of embryo culture in interspecific hybridization, use of haploids in plant improvement, and somaclonal variation and somatic hybridization as new techniques for plant improvement. The reader is also introduced to plant cell culture, as well as somatic cell genetics of cereals and grasses, somatic cell fusion for inducing cytoplasmic exchange, uses of cell culture mutants, genetic transformation of plant cells by experimental procedures in the context of plant genetic engineering, and use of molecular biology techniques for recognition and modification of crop plant genotypes. This book will be a useful resource for scientists and plant breeders interested in applying somatic cell genetics for crop improvement.