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Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Reach beyond your own personal experience and knowledge and rise faster and farther with this “engaging and practical” leadership guide (Stephen R. Covey, author of The 7 Habits of Highly Effective People). Being an effective leader means making decisions. But many of those decisions may pertain to situations or information you aren’t be familiar with. What is a leader to do then? You have to make choices about the future for yourself, your team, and your organization. You need to get your team aligned in the same direction while building key relationships both internally and externally. And, you have to do all this while you maintain your sanity among competing demands. Leadership expert Dave Jennings doesn’t ask you to change your leadership skill set. He invites you to change your mind set about how you approach life when you are in over your head—sometimes way over your head. Catapulted is a “great read” that will provide you with the tools to succeed in situations that go beyond your comfort zone. Get ready to fly (Gary Bowen, chief financial officer, OGIO International).
LIFE Magazine is the treasured photographic magazine that chronicled the 20th Century. It now lives on at LIFE.com, the largest, most amazing collection of professional photography on the internet. Users can browse, search and view photos of today’s people and events. They have free access to share, print and post images for personal use.
As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology. - Documents many diverse uses for confocal microscopy in disciplines that broadly span biology - The methods presented include shortcuts and conveniences not included in the initial publications - Techniques are described in a context that allows comparisons to other related methodologies - Methodologies are laid out in a manner that stresses their general applicability and reports their potential limitations
This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.
Spiritual Revolution will shake the way you think about the "supernatural" power of God-and your role in combating the counterfeit signs and wonders of today's cults. Spiritual Revolution contains stories, insights, and practical suggestions about how you can aggressively choose to live in the supernatural power of God. In this powerful book filled with facts and personal experiences, you will clearly discover the difference between: Churchianity & Christianity. Godly power & satanic control. Fact & truth. Supernatural visitations & occult experiences. Christians no longer need to sit idly by while the world is bombarded with new age practices, witchcraft, and satanism. No matter who you are or where you live, you play a vital role in establishing God's plan for the earth. Join the Spiritual Revolution! Author, teacher, and prophet Patricia King sounds the alarm for believers to entertain angelic creatures and slay the dragons. She leads you into the spiritual dimension where you will discover the glorious "Third Heaven" through the power of the Holy Spirit. Enlist in the Spiritual Revolution and you will be transformed into a powerful witness throughout an eternal lifetime. Book jacket.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. - Basic Principles, Specialized Uses, and Genetic Applications - LCM and its application in genomics and proteomics - Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections - Noncontact laser catapulting for the functional genomics and proteomics - Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more
Why literally shouldn't be taken literally. Why Americans think home in on something is a mistake and Brits think hone in is. Is it OK to spell OK okay? What's wrong with hence why? Was Alanis Morrisette ever ironic? Fowler's Dictionary of Modern English Usage is the world-famous guide to English usage, loved and used by writers, editors, and anyone who values correct English since it first appeared in 1926. Fowler's gives comprehensive and practical advice on complex points of grammar, syntax, punctuation, style, and word choice. Now enlarged and completely revised to reflect English usage in the 21st century, it provides a crystal-clear, authoritative picture of the English we use, while illuminating scores of usage questions old and new. International in scope, it gives in-depth coverage of both British and American English usage issues, with reference also to the English of Australia, Canada, India, New Zealand, and South Africa. The thousands of authentic examples in the book vividly demonstra
Tough times don't last but tough people do! Perseverance breaks resistance! You need to be inspired for action and for success. Success does not come on a silver plate but it belongs to those that have the staying power. This book is for all that are determined to stay in the game until they attain their goals and purpose for life.