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Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups.This book will be an essential laboratory reference for insect pathologists.Features include:* Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens* Details of the practical knowledge needed by beginners to apply the techniques* Chapters written by an international group of experts* Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques* Provides extensive supplemental literature and recipes for media, fixatives and stains
The 38 chapters of this Field Manual provide the tools required for planning experiments with entomopathogens and their implementation in the field. Basic tools include chapters on the theory and practice of microbial control agents, statistical design of experiments, equipment and application strategies. The major pathogen groups are covered in individual chapters (virus, bacteria, protozoa, fungi, nematodes). Subsequent chapters deal with the impact of naturally occurring and introduced exotic pathogens and inundative application of microbial control agents. The largest section of the Manual is composed of 21 chapters on the application and evaluation of entomopathogens in a wide range of agricultural, forest, domestic and aquatic habitats. Mites and slugs broaden the scope of the book. Supplementary techniques and media for follow-up laboratory studies are described. Three final chapters cover the evaluation of Bt transgenic plants, resistance to insect pathogens and strategies to manage it, and guidelines for evaluating the effects of MCAs on nontarget organisms. Readership: Researchers, graduate students, practitioners of integrated pest management, regulators, those doing environmental impact studies. The book is a stand-alone reference, but is also complementary to the laboratory-oriented Manual of Techniques in Insect Pathology and similar comprehensive texts.
1. Initial Handling and Diagnosis of Diseased Invertebrates / Lawrence A. Lacey and Leellen Solter -- 2. Basic Techniques in Insect Virology / Karolin E. Eberle, Jorg T. Wennmann, Regina G. Kleespies and Johannes A. Jehle -- 3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli. / Tanja W. Fisher and Steven F. Garczynski -- 4. Bioassay of Bacterial Entomopathogens Against Insect Larvae / Maureen O'Callaghan, Travis R. Glare and Lawrence A. Lacey --5. Bacteria for use Against Soil-Inhabitating Insects / Albrecht M. Koppenhofer, Trevor A. Jackson, and Michael G. Klein -- 6. Identification of Entomopathogenic Fungi / Richard A. Humber -- 7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales / G. Douglas Inglis, Juerg Enkerli, and Mark S. Goettel -- 8. Mass Production of Entomopathogenic Hypocreales / Stefan T. Jaronski and Mark A. Jackson -- 9. Methods for the Study of Entomophthorales / Ann E. Hajek, Bernard Papierok, and Jurg ...
Insect Pathology is designed for a broad spectrum of readers. Is should be useful to students, lecturers, and researchers requiring information about the principles in insect pathology and the biology of pathogens. It should serve as a resource for specialists to learn about other insect pathogen systems, for generalists to become aware of advances in insect pathology, and for scientists and students, beginning or otherwise, interested in learning about insect pathology. This book was originally intended to update the 1949 test by E. A. Steinhaus entitled Principles of Insect Pathology. The purpose for this book was twofold: To serve (1) as a text for an insect pathology and/or biological control class and (2) as a comprehensive reference source. Because this book summarizes much of the available information, its usefulness as a textbook for an insect pathology class is apparent. Although the literature citations are extensive, they are far from complete. The literature in insect pathology is voluminous and for the past decade has been expanding at an almost exponential rate. A complete review of the literature is beyond the scope of the book, and an omission of a reference does not preclude its importance. Our citations, however, should serve as a good starting point for those who wish to obtain further information. We have attempted to cover equally all subdisciplines, but shortcomings are unavoidable. For these, we take full responsibility.
Insect Pathology: An Advanced Treatise, Volume 2 reviews the significant progress that has been made in insect pathology, as well as the major research trends in the field. This book is concerned with bacterial, fungus, protozoan, and parasitic diseases, along with the epizootiology of infectious diseases, diagnosis, methodology, and microbial control. Organized into 17 chapters, this volume begins with a brief consideration of the taxonomy of entomogenous bacteria, including common problems of bacterial taxonomy as a whole. The book then discusses diseases caused by certain spore-forming bacteria, such as clostridial pathogens and pathogens of the genus Bacillus. The text also explains milky diseases and their development within the insect host; nonsporulating bacterial pathogens that cause diseases in insects; and infections caused by Coelomomyces and Entomophthorales. The book also introduces the reader to insect diseases caused by hyphomycetous Fungi Imperfecti (Deuteromycetes) not usually associated in nature with a perfect stage, diseases associated with Cordyceps, and infections attributed to parasites belonging to the group Sporozoa. This book is essential reading for entomologists.
Principles of Insect Pathology, a text written from a pathological viewpoint, is intended for graduate-level students and researchers with a limited background in microbiology and in insect diseases. The book explains the importance of insect diseases and illuminates the complexity and diversity of insect-microbe relationships. Principles of Insect Pathology combines the disciplines of microbiology (virology, bacteriology, mycology, protozoology), pathology, and immunology within the context of the insect host, providing a format which is understandable to entomologists, microbiologists, and comparative pathologists.
Microbial Control of Insect and Mite Pests: From Theory to Practice is an important source of information on microbial control agents and their implementation in a variety of crops and their use against medical and veterinary vector insects, in urban homes and other structures, in turf and lawns, and in rangeland and forests. This comprehensive and enduring resource on entomopathogens and microbial control additionally functions as a supplementary text to courses in insect pathology, biological control, and integrated pest management. It gives regulators and producers up-to-date information to support their efforts to facilitate and adopt this sustainable method of pest management. Authors include an international cadre of experts from academia, government research agencies, technical representatives of companies that produce microbial pesticides, agricultural extension agents with hands on microbial control experience in agriculture and forestry, and other professionals working in public health and urban entomology. - Covers all pathogens, including nematodes - Addresses the rapidly progressing developments in insect pathology and microbial control, particularly with regard to molecular methods - Demonstrates practical use of entomopathogenic microorganisms for pest control, including tables describing which pathogens are available commercially - Highlights successful practices in microbial control of individual major pests in temperate, subtropical, and tropical zones - Features an international group of contributors, each of which is an expert in their fields of research related to insect pathology and microbial control