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PHAs are synthesized by many microorganisms to serve as intracellular carbon storage molecules. In some bacterial strains, PHB can account for up to 80% of cell mass. In addition to its application in the packaging sector, PHB also has great potential as an intermediate in the production of hydrocarbon fuels. PHB can be thermally depolymerized and decarboxylated to propene which can be upgradedto hydrocarbon fuels via commercial oligomerization technologies. Cupriavidus necator is the microorganism that has been most extensively studied and used for PHB production on an industrial scale; However the substrates used for producing PHB are mainly fructose, glucose, sucrose, fatty acids, glycerol, etc., which are expensive. In this study, we demonstrate production of PHB from a processrelevant lignocellulosic derived sugar stream, i.e., saccharified slurry from pretreated corn stover. The strain was first investigated in shake flasks for its ability to utilize glucose, xylose and acetate. In addition, the strain was also grown on pretreated lignocellulose hydrolyzate slurry and evaluated in terms of cell growth, sugar utilization, PHB accumulation, etc. The mechanism ofinhibition in the toxic hydrolysate generated by the pretreatment and saccharification process of biomass, was also studied.
Concerns over dwindling fossil fuel reserves and impending climate changes have focused attention worldwide on the need to discover alternative, sustainable energy sources and fuels. Biofuels, already produced on a massive industrial scale, are seen as one answer to these problems. However, very real concerns over the effects of biofuel production on food supplies, with some of ht recent increases in worldwide food costs attributable to biofuel production, have lead to the realization that new, non-food substrates for biofuel production must be bought online. This book is an authoritative, comprehensive, up-to-date review of the various options under development for the production of advanced biofuels as alternative energy sources. A general overview and introductory chapters for each section place the field in the context as well as provide essential basic notions for the more general reader. Accomplished, internationally recognized experts carrying out research on individual focus areas contribute specific technical chapters detailing present progress and future prospects.
Petroleum-based industrial products have gradually replaced products derived from biological materials. However, biologically based products are making a comebackâ€"because of a threefold increase in farm productivity and new technologies. Biobased Industrial Products envisions a biobased industrial future, where starch will be used to make biopolymers and vegetable oils will become a routine component in lubricants and detergents. Biobased Industrial Products overviews the U.S. land resources available for agricultural production, summarizes plant materials currently produced, and describes prospects for increasing varieties and yields. The committee discusses the concept of the biorefinery and outlines proven and potential thermal, mechanical, and chemical technologies for conversion of natural resources to industrial applications. The committee also illustrates the developmental dynamics of biobased products through existing examples, as well as products still on the drawing board, and it identifies priorities for research and development.
Research and innovation in the life sciences is driving rapid growth in agriculture, biomedical science, information science and computing, energy, and other sectors of the U.S. economy. This economic activity, conceptually referred to as the bioeconomy, presents many opportunities to create jobs, improve the quality of life, and continue to drive economic growth. While the United States has been a leader in advancements in the biological sciences, other countries are also actively investing in and expanding their capabilities in this area. Maintaining competitiveness in the bioeconomy is key to maintaining the economic health and security of the United States and other nations. Safeguarding the Bioeconomy evaluates preexisting and potential approaches for assessing the value of the bioeconomy and identifies intangible assets not sufficiently captured or that are missing from U.S. assessments. This study considers strategies for safeguarding and sustaining the economic activity driven by research and innovation in the life sciences. It also presents ideas for horizon scanning mechanisms to identify new technologies, markets, and data sources that have the potential to drive future development of the bioeconomy.
This book is a printed edition of the Special Issue "Advances in Polyhydroxyalkanoate (PHA) Production" that was published in Bioengineering
This book is open access under a CC BY 4.0 license. This book defines the new field of "Bioeconomy" as the sustainable and innovative use of biomass and biological knowledge to provide food, feed, industrial products, bioenergy and ecological services. The chapters highlight the importance of bioeconomy-related concepts in public, scientific, and political discourse. Using an interdisciplinary approach, the authors outline the dimensions of the bioeconomy as a means of achieving sustainability. The authors are ideally situated to elaborate on the diverse aspects of the bioeconomy. They have acquired in-depth experience of interdisciplinary research through the university’s focus on “Bioeconomy”, its contribution to the Bioeconomy Research Program of the federal state of Baden-Württemberg, and its participation in the German Bioeconomy Council. With the number of bioeconomy-related projects at European universities rising, this book will provide graduate students and researchers with background information on the bioeconomy. It will familiarize scientific readers with bioeconomy-related terms and give scientific background for economists, agronomists and natural scientists alike.
Living systems synthesize seven different classes of polymers. They provide structure and form for cells and organisms, function as catalysts and energy storage and carry the genetic information. All these polymers possess technically interesting properties. Some of these biopolymers are already used commercially. This special volume of Advances in Biochemical Engineering/Biotechnology comprises 10 chapters. It gives an overview of the water insoluble biopolyesters, in particular of the microbially synthesized poly-hydroxyalkanoate (PHA) family. It reports the state of the art of metabolism, regulation and genetic background, the latest advances made in genetic optimization of bacteria, "construction" of transgenic plants and in vitro synthesis by means of purified enzymes. Furthermore, it describes relevant technologies and evaluates perspectives concerning increasing the economic viability and competitiveness of PHA and discusses applications in medicine, packaging, food and other fields.
With the increasing awareness and concern about the dependency on fossil resources and the depletion of crude oil reserves, experts from industrial biotechnology, renewable resources, green chemistry, and biorefineries are stimulating the transition from the fossil-based to the bio-based economy. This text confronts scientific and economic challenges and strategies for making this crucial transition. Renewable Resources for Biorefineries is the work of a strongly interdisciplinary authorship, offering perspectives from biology, chemistry, biochemical engineering, materials science, and industry. This unique approach provides an opportunity for a much broader coverage of biomass and valorisation than has been attempted in previous titles. This book also represents the fundamentally important technical and policy aspects of a bio-based economy, to ground this important science in a realistic and viable economic framework. Chapters in this book cover a diverse range of topics, including: advanced generation bioenergy sectors; biobased polymers and materials; chemical platform molecules; industrial crops and biorefineries; financing and policy for change; and valorisation of biomass waste streams. This is an ideal book for upper level undergraduate and postgraduate students taking modules on Renewable resources, green chemistry, sustainable development, environmental science, agricultural science and environmental technology. It will also benefit industry professionals and product developers who are looking to improve economic and environmental ways to utilise renewable resources in current and future biorefineries.
Introduction to Bioplastics Engineering is a practical, user-friendly reference for plastics engineers working with biopolymers and biodegradable plastics that addresses topics that are required for the successful development of cohesive bioplastic products. While there has been considerable demand for the use of bioplastics in industry, processing these bioplastics is a big challenge. The book provides plastics engineers and researchers with a fundamental, practical understanding of the differences between bioplastics and biodegradable polymers, along with guidance on the different methods used to process bioplastics. The book also covers additives and modifiers for biopolymers and their effect on properties. Examples include commercial applications of bioplastics, current bioplastics being developed, and future trends in the industry. This enables engineers, researchers, technicians, and students to understand the decisive relationship between different processing techniques, morphology, mechanical properties, and the further applications of bio-based polymers. The book presents a true engineering approach for the industry on the processing of biopolymers and biodegradable plastics – discussing the ease of use of the polymer, mechanical and thermal properties, rate of biodegradation in particular environments, and pros and cons of particular bioplastics. - Enables engineers, researchers, technicians, and students to understand the decisive relationship between different processing techniques, morphology, mechanical properties, and the further applications of bio-based polymers. - Covers additives and modifiers for biopolymers and their effect on properties - Includes examples that illustrate the commercial applications of bioplastics, current bioplastics being developed, and future trends in the industry
This book focuses on the basic science recently produced in Brazil for the improvement of sugarcane as a bioenergy crop and as a raw material for 2nd generation bioethanol production. It reports achievements that have been advancing the science of cell walls, enzymes, genetics, and sustainability related to sugarcane technologies and give continuity to the research reported in the “Routes to Cellulosic Ethanol”, from Springer. The Introduction (Chapter I) explains how the National Institute of Science and Technology of Bioethanol, founded in 2008 in Brazil, became part of the main international initiatives that started to search for forms to use biomass for bioethanol production in Brazil, US and Europe. Part I reports the advances in plant cell wall composition, structure and architecture, and physical characteristics of sugarcane biomass. These discoveries are opening the way to increased efficiency of pretreatments and hydrolysis, being therefore important information for 2nd generation processes as well as for biorefinery initiatives. Part II focuses on the discovery and characterization of hydrolases from microorganisms that could be used in industrial processes. Recent advances in the search for hydrolases using metagenomics is reported. A great number of genes and enzymes from microorganisms have been discovered, affording improvement of enzyme cocktails better adapted to sugarcane biomass. Part III reports two key issues in the process of 2G ethanol, pentose fermentation and sugarcane genetics. These are the discoveries of new yeast species capable of producing ethanol more efficiently from xylose and the advances made on the sugarcane genetics, a key issue to design varieties adapted to 2G ethanol production. Part IV approaches sustainability through two chapters, one discussing the sustainability of the sugarcane agricultural and environmental system and another discussing how national and mainly international policies of Brazil regarding 2G ethanol production affected the country’s strategies to establish itself as an international player in renewable energy area.