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Date palm trees are exposed to infection with different diseases caused by many soil-borne pathogenic fungi that may cause considerable losses in the offshoots and trees. This study was carried out on five cvs. of date palm during two years in Aswan and New Valley governorates. Isolation and identification of associated fungi showed that the most frequent fungi were Fusarium oxysporum, F. moniliforme and Thielaviopsis paradoxa while the least frequent was Phytophthora sp. The cultivars were differed susceptible to infection by the isolated pathogenic fungi. Barhey cultivar was the most susceptible to infection, followed by Gondaila, Siwey and Partmoda cultivars, while Sakkoty cultivar was the least susceptible. Seven streptomycetes were isolated from the rhizosphere soil of healthy date palm cultivated in Aswan governorate. Streptomycetes were namated viz. Streptomyces bobilii (isolate A1, A5 and A7), Streptomyces grisiobrunneus (isolate A2 and A3), Streptomyces albolongus (isolate A4 and A6). Three isolates A1, A6 and A3 of them gave highest results in vitro and in greenhouse experiments against soil-borne pathogenic fungi compared with negative and positive control. The obtained results showed that Streptomyces had the highest efficacy on root rots of date palm. This is the first study done to use actinomycetes against soil-borne pathogenic fungi that caused root rots of date palm.
The decline disease in date palm (Phoenix dactylifera L.) has become one of the most severe problems in date palm offshoots of the Egypt. Our previous studies have established that Lasiodiplodia theobromae (Syn: Botryodiplodia theobromae) is one of the fungi causing root rot diseases. This study was carried out in three governorates Bani-suef, Behara and Kaluobia. A survey of governorates was conducted in 2011 and 2012 to collect the root-rotted samples from rhizosphere of declined date palm offshoot and trees. Behara was the most affected governorate with 25.5% disease severity, while in Kaluobia it was 14.5% and in Bani-suef it was 13%. Bartamoda cultivar was the least susceptible to infection by the pathogenic fungus followed by Sakoty, Siwey, Sammany and Zaghloul. L. theobromae was most abundantly isolated fungal pathogen. Colony growth of L. theobromae was maximum at 35ºC and optimum temperature for disease incidence and severity was 30ºC. Relative humidity percentage of 70% is best for the growth of L. theobromae, while the optimum Rh% for disease incidence and severity it was 60%. Water salinity (ECw) at the concentration 15.63 ds/m2 was reduction linear growth of L. theobromae to 17.03%, while at water salinity concentration increased the severity of all cultivars tested. Root exudates from Bartamoda cv. the most effective on growth rate of L. theobromae at concentration 10%, it was reduction linear growth to 41.30%. Jojoba oil proved the best bioagent in vitro suppressing the growth of L. theobromae showing reduction of linear growth to 83.33% at 500 ppm. Plant guard was found the second most effective reduction to 52.96%. Marjoram showed the least efficacy against L. theobromae in all concentrations. Experiments in greenhouse to control the root rot disease proved the Jojoba oil gave the best results to decreased disease severity, followed by Plant guard and the least was Marjoram against L. theobromae at all cultivars tested.
Fusarium wilt of banana: some history and current status of the disease; Importante of fusarium wilt in different banana-growing regions; Taxonomy of fungi in the genus fusarium with emphasis on fusarium oxysporum; Genetic exchange within sexual and asexual populations of the genus fusarium; Molecular genetics of plant pathogenic fusarium oxysporum; Using karyotype variability to investigate the origins and relatednes of isolates of fusarium oxysporum f. sp. cubense; Population biology of fusarium oxysporum f. sp. cubense; Biological control of diseases caused by fusarium oxysporum; Influence of mineral nutrition on fusarium wilt: a proposed mechanism involving cell water relations; Host responses to the pathogen; Banana breeding and fusarium wilt; Breeding bananas and plantains for resistance to fusariu m wilt: the track record; Somaclonal resistance in cavendish banana to fusarium wilt; Baseline tissue and cell culture studies for use in banana improvement schemes.
The papers contained in this book were presented at a NATO Advanced Research Workshop (ARW) held at Cape Sounion, Athens, Greece, 19-24 May, 1991. The twenty-eight more comprehensive papers represent the key subjects of the ARW covered by invited speakers. The thirty-four short papers pre sented in a research format are contributions of those invited to participate in the ARW. There was a total of 70 participants from 21 countries. The objectives of the ARW were as follows: to review current knowledge of biological control of plant diseases and plant parasitic nematodes, with emphasis on mechanisms at the molecular, cellular, organismal, and ecosystem level; to examine and expand on current concepts and synthesize new concepts; to identify and prioritize limitations in the use of biological control for plant diseases and nematodes and the scientific research needed to overcome these limitations; and to develop strategies for biological control through management of resident agents or introduction of natural or modified agents.
This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.
The Theory and Practice of Biological Control covers conventional biological control achievement in the major crop types and in public health problems. Composed of five sections encompassing 28 chapters, this book discusses the basic information concerning developments in other biologically based alternatives to chemical pesticides. The first two sections discuss the philosophy, theory, scope, history, and the biological and ecological bases of biological control. These sections also deal with the impact of predators and the host relationships of parasitoids and pathogens. The following section presents the methodological aspects of biological control. Discussions on the variability of natural enemies as encountered in biological control work; the fitness of individuals and populations; the ways fitness is being or can be influenced by importation procedures; and the ability of imported natural enemies to adapt to the new environment are included. The fourth section outlines the accomplishments of conventional biological control in various types of crops, forests, and public health areas. Lastly, the various components of integrated pest control other than conventional biological control that forms the essential ways used in the integrated control approach are covered in the last section of the book. This book is an ideal source for plant pathologists and researchers, microbiologists, parasitologists, and public health professionals.
“Microbial Enzymes: Roles and applications in industry” offers an essential update on the field of microbial biotechnology, and presents the latest information on a range of microbial enzymes such as fructosyltransferase, laccases, amylases, lipase, and cholesterol oxidase, as well as their potential applications in various industries. Production and optimisation technologies for several industrially relevant microbial enzymes are also addressed. In recent years, genetic engineering has opened up new possibilities for redesigning microbial enzymes that are useful in multiple industries, an aspect that the book explores. In addition, it demonstrates how some of the emerging issues in the fields of agriculture, environment and human health can be resolved with the aid of green technologies based on microbial enzymes. The topics covered here will not only provide a better understanding of the commercial applications of microbial enzymes, but also outline futuristic approaches to use microbial enzymes as driver of industrial sustainability. Lastly, the book is intended to provide readers with an overview of recent applications of microbial enzymes in various industrial sectors, and to pique researchers’ interest in the development of novel microbial enzyme technologies to meet the changing needs of industry.
For the first time in over 20 years, a comprehensive collection of photographs and descriptions of species in the fungal genus Fusarium is available. This laboratory manual provides an overview of the biology of Fusarium and the techniques involved in the isolation, identification and characterization of individual species and the populations in which they occur. It is the first time that genetic, morphological and molecular approaches have been incorporated into a volume devoted to Fusarium identification. The authors include descriptions of species, both new and old, and provide protocols for genetic, morphological and molecular identification techniques. The Fusarium Laboratory Manual also includes some of the evolutionary biology and population genetics thinking that has begun to inform the understanding of agriculturally important fungal pathogens. In addition to practical “how-to” protocols it also provides guidance in formulating questions and obtaining answers about this very important group of fungi. The need for as many different techniques as possible to be used in the identification and characterization process has never been greater. These approaches have applications to fungi other than those in the genus Fusarium. This volume presents an introduction to the genus Fusarium, the toxins these fungi produce and the diseases they can cause. "The Fusarium Laboratory Manual is a milestone in the study of the genus Fusarium and will help bridge the gap between morphological and phylogenetic taxonomy. It will be used by everybody dealing with Fusarium in the Third Millenium." --W.F.O. Marasas, Medical Research Council, South Africa
Global concern over the demerits of chemicals in agriculture has diverted the attention of researchers towards using the potential of PGPR in agriculture. This book contains many useful and important research papers pertaining to the use of bio-fertilizers and bio-fungicides for sustainable agriculture. This volume is presented in an easy-to-understand manner,with well-illustrated protocols on the production to commercialization of PGPR. The chapters on commercial potential, trade and regulatory issues among Asian countries are worthwhile additions. As such, this book will prove useful for students, researchers, teachers, and entrepreneurs in the area of PGPR and its allied fields.
Following the successful first edition, this revised and greatly expanded edition Tomato Diseases: Identification, Biology and Control is the definitive work on the diseases and disorders of the tomato. The tomato is the world's most widely produced vegetable. The number of diseases affecting the tomato is enormous: hundreds of bio-aggressors, more