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This book deals with theoretical and practical developments of IEF and offers detailed methodology for many of the commonly used procedures, such as IEF in gels. It is intended both as a reference guide and a practical manual.
Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics
First multi-year cumulation covers six years: 1965-70.
Fundamentals and Techniques
Biochemistry: The Chemical Reactions of Living Cells is a well-integrated, up-to-date reference for basic biochemistry, associated chemistry, and underlying biological phenomena. Biochemistry is a comprehensive account of the chemical basis of life, describing the amazingly complex structures of the compounds that make up cells, the forces that hold them together, and the chemical reactions that allow for recognition, signaling, and movement. This book contains information on the human body, its genome, and the action of muscles, eyes, and the brain. It also features: thousands of literature references that provide introduction to current research as well as historical background; twice the number of chapters of the first edition; and each chapter contains boxes of information on topics of general interest. -- Publisher description.
Biomedical Applications of Mass Spectrometry Edited by Clarence H. Suelter and J. Throck Watson This unusual text is not simply a compilation of mass spectrometric methods but provides, instead, insight into specific approaches mass spectroscopists use when applying the technique to a variety of biological problems. Each chapter provides guidance in using the appropriate methods for isolating and purifying the compound class prior to mass spectrometric analysis. Covered in-depth are the mass spectrometry of carbohydrates, peptide sequencing by mass spectrometry, mass spectrometry of nucleic acid components, and mass spectrometry in pharmacology. This definitive look at a growing facet of the science is an essential reference for biochemists, biological chemists, bioanalytical chemists, and students. 1990 (0-471-61303-7) 396 pp.
Trends in Analytical Chemistry, Volume 5 focuses on the advancements of processes, technologies, automation, and applications of analytical chemistry. The selection first offers information on graphics programming for the IBM PC using FORTRAN, PASCAL, and C, including graphics hardware system software, assembly language routines, and high level interface. The text then elaborates on the place of affinity chromatography in the production and purification of biomolecules from cultured cells and zone electrophoresis in open-tubular capillaries. Discussions focus on column and instrument design, applications, affinity chromatography in protein production from cells, and economic aspects of production and purification of proteins from cell cultures. The manuscript takes a look at polarographic and voltammetric techniques and their application to the determination of vitamins and coenzymes and activation analysis with charged particles. Topics include accelerators, principle of charged particle activation analysis, and applications. The text then examines the development of microbiological and immunological assays for antibiotics and the use of computer system for a small analytical research laboratory. The book is a dependable reference for readers interested in the trends in analytical chemistry.
This book provides a comprehensive review at the biochemical and molecular level of the processes and techniques that contribute to crop improvement. General topics include a historical perspective of the advancements in crop improvement; cultivar systematics and biochemical and molecular markers in crop improvement programs; the genetics of physiological and biochemical processes affecting crop yield; the genetics of photosynthesis, chloroplast, relevant enzymes, and mutations; osmoregulation/adjustment and the production of protective compounds in relation to drought tolerance; and the biochemistry of disease resistance, including elicitors, defense response genes, their role in the production of phytoalexins and other strategies against pathogens. Other topics include quality breeding (e.g., molecular gene structure, changing individual amino acids, enhancing nutritive value of proteins) and biotechnology/genetic engineering. Geneticists, biochemists, botanists, agricultural specialists and others involved in crop improvement and breeding should consider this volume essential reading.