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This book intends to be neither a complete survey of the field nor an exhaustive source of references. For these purposes, the use of the extensive compilation "Experimental Immunochemistry" by E. A. KABAT and M. M. MAYER (1962) or the excellent methodological textbook, "Methods in Immunology", by D. H. CAMPBELL, J. S. GARVEY, E. E. CREMER and D. H. SUSSDORF (1963), or the quite comprehensive series "Methods in Immunology and Immunochemistry" by C. A. WILLIAMS and M. W. CHASE (1967) are more suitable. The main purpose of this manual ist to provide students with a simple book which will introduce them to some frequently occurring problems in the three major sections of the immunochemistry of natural products. These are the isolation of the materials, the chemical analysis of the constituents and their structure, and, finally, the assays of the most important biological and immunological activities. In this manual the exercises are simplified and several shortcuts are taken in order to fit them into the framework of a teaching course. The introduction to each exercise gives a brief and elementary explanation of the reaction on which it is based. "Materials and Equipment" lists all tissues or cells, chemicals, glassware, and special equipment which must be available to carry out the exercise, although the very common laboratory tools are usually omitted from the list.
Includes subject section, name section, and 1968-1970, technical reports.
A timely, accessible survey of the multidisciplinary field of bioanalytical chemistry Provides an all in one approach for both beginners and experts, from a broad range of backgrounds, covering introductions, theory, advanced concepts and diverse applications for each method Each chapter progresses from basic concepts to applications involving real samples Includes three new chapters on Biomimetic Materials, Lab-on-Chip, and Analytical Methods Contains end-of-chapter problems and an appendix with selected answers
Partial antibody characterization indicates that specific channel catfish immunoglobulins are macroglobulins with characteristics like those of most fish immunoglobulins and similar to those of human immunoglobulin-M.
The present new version of this popular laboratory manual is at the same time the first one of this text in the English language - and this makes me even a little proud, as it reminds me of probably the first collection of monoclonal recipes in English, written by myself, which circulated for a couple of years in many laboratories. In the meantime many researchers have put enormous effort into improving methods for monoclonal antibody production. The proce dures have become more and more standardized and by this have more and more lost the character of magic secrets. Hinrich Peters and Horst Baumgarten, who had followed this good tradition already in the previous edition, written in German, suc ceeded in making laboratory tricks teachable. They had contributed their own experiences in cell culture and immunology, and were able to engage a number of experienced authors to contribute to the work. They were all willing to follow the general concept of this book, which contains a brief theoretical background for the methods described and presents the procedures in a highly organized structure. So the book has retained its shape as a "cook-book", which I especially like.