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DNA Barcoding has been promoted since 2003 as a new, fast, digital genomics-based means of identifying natural species based on the idea that a small standard fragment of any organism's genome (a so-called "micro-genome") can faithfully identify and help to classify every species on the planet. The fear that species are becoming extinct before they have ever been known fuels barcoders, and the speed, scope, economy and "user-friendliness" claimed for DNA barcoding, as part of the larger ferment around the "genomics revolution", has also encouraged promises that it could inspire humanity to reverse its biodiversity-destructive habits. This book is based on six years of ethnographic research on changing practices in the identification and classification of natural species. Informed both by Science and Technology Studies (STS) and the anthropology of science, the authors analyse DNA barcoding in the context of a sense of crisis concerning global biodiversity loss, but also the felt inadequacy of taxonomic science to address such loss. The authors chart the specific changes that this innovation is propelling in the collecting, organizing, analyzing, and archiving of biological specimens and biodiversity data. As they do so they highlight the many questions, ambiguities and contradictions that accompany the quest to create a genomics-based environmental technoscience dedicated to biodiversity protection. They ask what it might mean to recognise ambiguity, contradiction, and excess more publicly as a constitutive part of this and other genomic technosciences. Barcoding Nature will be of interest to students and scholars of sociology of science, science and technology studies, politics of the environment, genomics and post-genomics, philosophy and history of biology, and the anthropology of science.
Shortly, this book is the written up-graded version of the topics discussed during the Small Meeting of the 2nd International School Congress: Natural Resources, Sustainability and Humanity, held in Braga, Portugal, 5-8 May 2010 with the diverse participation of scientists, educators and governmental representatives. The Earth hosts an immense ecosystem, colonized by millions of species for billions of years but only for a few tens of thousands of years by humans. Environmental history tells though that it was humankind that shaped the environment as no other species. History, geography, religion and politics among other reasons have differentiated populations with respect to access to safe food and water, education, health, and to space and natural resource utilization. The globalization era of trade, information and communication is shortening distances and increasing overall wealth, but, as is pointed out in this book, it is also contributing to the propagation of diseases, and to the modification or even destruction of native ecosystems by exotic invasive species. Man is the only species that has the perception of its history, evolution, of the consequences of its decisions, and that there is a future ahead. It is also the only species that has the potential to change it. This awareness can be a source of anxiety and contradictory behaviours, but it is also the key to changing attitudes towards the construction of a common sustainable home, by committed education, interdisciplinary approaches, mobilization and empowerment of people and political consonant actions.
This book presents a comprehensive overview of DNA barcoding and molecular phylogeny, along with a number of case studies. It discusses a number of areas where DNA barcoding can be applied, such as clinical microbiology, especially in relation to infection management; DNA database management; and plant -animal interactions, and also presents valuable information on the DNA barcoding and molecular phylogeny of microbes, algae, elasmobranchs, fishes, birds and ruminant mammals. Furthermore it features unique case studies describing DNA barcoding of reptiles dwelling in Saudi Arabian deserts, genetic variation studies in both wild and hatchery populations of Anabas testudineus, DNA barcoding and molecular phylogeny of Ichthyoplankton and juvenile fishes of Kuantan River in Malaysia, and barcoding and molecular phylogenetic analysis of indigenous bacteria from fishes dwelling in a tropical tidal river. Moreover, since prompt identification and management of invasive species is vital to prevent economic and ecological loss, the book includes a chapter on DNA barcoding of invasive species. Given its scope, this book will appeal not only to researchers, teachers and students around the globe, but also to general readers.
A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used with DNA barcodes. These methods include the latest information on techniques for generating, applying, and analyzing DNA barcodes across the Tree of Life including animals, fungi, protists, algae, and plants. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, DNA Barcodes: Methods and Protocols aids scientists in continuing to study methods from wet-lab protocols, statistical, and ecological analyses along with guides to future, large-scale collections campaigns.
Drawing from meticulous research and captivating storytelling, the author unveils the intricate relationship between various species and their habitats. We journey through lush rainforests, vibrant coral reefs, and vast grasslands, discovering the delicate balance that sustains our fragile planet. Throughout the pages, the author unravels the intricate factors contributing to the decline in biodiversity. From deforestation and habitat destruction to pollution and climate change, these forces intertwine to bring about devastating consequences for countless species. As we navigate through the chapters, the author highlights the real-life ecological havoc caused by human activities. We witness the dire plight of endangered species, the loss of crucial biodiversity hotspots, and the looming shadow of extinction. The author intricately addresses the complex symbiosis of life and showcases the irreplaceable value of each individual species. However, the outlook is not entirely bleak. The author highlights inspiring conservation initiatives, depicting the unwavering dedication of scientists, activists, and communities in safeguarding nature's legacy. This flicker of hope guides us towards potential solutions and highlights the urgent need to act. The Silent Spring Revisited is a wake-up call, urging us to pause and reflect on the impact of our actions on the intricate tapestry of life. This book resonates deeply, stirring an appreciation for the extraordinary beauty and resilience of our planet's biodiversity. It galvanizes us to embrace our role as stewards of the Earth, to implement change, and to ensure a future where spring remains a symphony of life.
A comprehensive and accessible resource covering all aspects of forensic and legal medicine. The text provides a foundation for those working in both the clinical and forensic aspects of care and will also be an asset to those involved in the police or judicial systems. Including clear guidelines for practical applications, and further enhanced by its many illustrations and case examples, this text is a valuable resource in an increasingly complex field. The authoritative work is writtenn by those who have extensive experience for a wide audience including, but not limited to, forensic pathologists, general pathologists, pediatric pathologists, forensic physicians, forensic scientists, coroners, emergency department physicians, judges and legal practitioners.
Natural Products and Drug Discovery: An Integrated Approach provides an applied overview of the field, from traditional medicinal targets, to cutting-edge molecular techniques. Natural products have always been of key importance to drug discovery, but as modern techniques and technologies have allowed researchers to identify, isolate, extract and synthesize their active compounds in new ways, they are once again coming to the forefront of drug discovery. Combining the potential of traditional medicine with the refinement of modern chemical technology, the use of natural products as the basis for drugs can help in the development of more environmentally sound, economical, and effective drug discovery processes. Natural Products & Drug Discovery: An Integrated Approach reflects on the current changes in this field, giving context to the current shift and using supportive case studies to highlight the challenges and successes faced by researchers in integrating traditional medicinal sources with modern chemical technologies. It therefore acts as a useful reference to medicinal chemists, phytochemists, biochemists, pharma R&D professionals, and drug discovery students and researchers. - Reviews the changing role of natural products in drug discovery, integrating traditional knowledge with modern molecular technologies - Highlights the potential future role of natural products in preventative medicine - Supported by real world case studies throughout
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Sharks are only a small proportion of world recorded fish landings, but they are a versatile and valuable resource. They sustain important fisheries in several countries and are a cheap but valuable source of protein for coastal communities dependent on subsistence fisheries. Sharks are exploited for their meat, fins, teeth, cartilage, liver and other internal organs. This report details species used and methods of preparation for various purposes.