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Autoradiography in Biology and Medicine focuses on the applications of autoradiography in medicine and biology, including photographic processes, emulsions, and response of films to isotopes and x-rays. The book first offers information on the meaning of autoradiography, including the etymology of autoradiography, photographic emulsion as a scientific instrument, and the relationship of biologists and autoradiography. The publication then examines the photographic process and comparative response of commercial and scientific emulsions. Discussions focus on fixation, washing, emulsion, response of films to electrons, x-rays, and gamma rays, and response of films to beta ray spectra of isotopes. The text takes a look at commercial photographic materials for autoradiography, autoradiographic image, and resolution. The manuscript then reviews the estimation of dose and exposure time, sources of error, and techniques. Topics include chemical variables, estimation of exposure time, errors occurring during the preparation of the autoradiogram for exposure, and contour autoradiography. The book is a valuable reference for readers interested in autoradiography.
Receptor autoradiography is an invaluable technique that is widely used to localize and characterize peptide and neurotransmitter binding sites. Its extensive use reflects the utility of the technique and the important advantages it offers over conventional biochemical procedures. This instructive work makes receptor autoradiography more accessible by giving a detailed account of the methodology and its application in the analysis of receptors in the brain and peripheral organs. These examples are not exhaustive but illustrate the versatility of the technique as well as its potential pitfalls. The book provides an introduction to the receptor concept and gives detailed information on the theory and procedures involved in the autoradiographic localization and quantification of radiolabelled ligand binding sites. This volume will be an essential tool for neuroscientists, pharmacologists, histochemists, neuropathologists, researchers in the pharmaceutical industry, and students of the biological sciences.
The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
In 1957 two young scientists, Matthew Meselson and Frank Stahl, produced a landmark experiment confirming that DNA replicates as predicted by the double helix structure Watson and Crick had recently proposed. It also gained immediate renown as a “most beautiful” experiment whose beauty was tied to its simplicity. Yet the investigative path that led to the experiment was anything but simple, Frederic L. Holmes shows in this masterful account of Meselson and Stahl’s quest. This book vividly reconstructs the complex route that led to the Meselson-Stahl experiment and provides an inside view of day-to-day scientific research--its unpredictability, excitement, intellectual challenge, and serendipitous windfalls, as well as its frustrations, unexpected diversions away from original plans, and chronic uncertainty. Holmes uses research logs, experimental films, correspondence, and interviews with the participants to record the history of Meselson and Stahl’s research, from their first thinking about the problem through the publication of their dramatic results. Holmes also reviews the scientific community’s reception of the experiment, the experiment’s influence on later investigations, and the reasons for its reputation as an exceptionally beautiful experiment.
Annotation Contains 42 seminal papers illustrating advances in cell biology, along with brief commentaries that place the papers in historical and intellectual context. All papers are studies of eukaryotes, and are grouped according to themes of genome organization and replication, transcription, nuclear envelope and nuclear import, mitosis and cell cycle control, cell membrane and extracellular matrix, protein synthesis and membrane traffic, and cytoskeleton. Lacks a subject index. Gall teaches embryology at the Carnegie Institution. McIntosh teaches cell biology at the University of Colorado. Annotation c. Book News, Inc., Portland, OR (booknews.com).
Provides an introduction to the use of radioactivity in the bioscience laboratory. The text covers general aspects of radioactivity, methods for the detection of radioactivity, radioisotope protocols used to study key cellular processes, and a summary of legislative requirements in the US and European Union. Guidance on safe handling and detailed recipes are provided.
The British Nuclear Medicine Society celebrates its 50th Anniversary with this booklet, which reflects the research of many of the pioneers in the use of radionuclides for the diagnosis and therapy of human disease. Since 1949 there have been remarkable advances in radionuclide techniques and imaging equipment: from the first devices “home-made” in the many physics departments throughout the UK, to the sophisticated multimodality imagers now in everyday use in Nuclear Medicine. The BNMS has been instrumental in promoting the use of radionuclide techniques in the investigation of pathology by supporting and providing education, research and guidelines on the optimum use of radiation to help patients. The future of Nuclear Medicine is bright, thanks to improved imaging resolution, new radiopharmaceuticals, and new diagnostic and therapeutic techniques and procedures.