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Applied Molecular Biotechnology: The Next Generation of Genetic Engineering explains state-of-the-art advances in the rapidly developing area of molecular biotechnology, the technology of the new millennium. Comprised of chapters authored by leading experts in their respective fields, this authoritative reference text:Highlights the latest omics-ba
Even though molecular biology has long been a basic tool in biomedical research, scientists still face the question of why certain molecular biology methods are used for certain experiments. To unlock the mystery, one must first understand the principles behind the methods. Unfortunately, very few molecular biology books have successfully provided satisfactory explanations. This book intends to fill this void by offering topics ranging from basic knowledge to the current state of the art in applied molecular biology. The principles and applications related to each technique included in the text are all described in full detail.
The book will be useful for undergraduate students as a supplementary/reference text in the field of molecular biotechnology.
The filamentous fungi are perhaps unique in the diversity of their metabolic activities. This includes biosynthetic as well as degradative activities, many is to provide an of which are of industrial interest. The objective of this text up-to-date and broad review which emphasizes the genetic and molecular biological contribution in the field of fungal biotechnology. This text begins with an overview of the tools and methodologies involved which, to a large extent, have been developed in the model filamentous fungus Aspergillus nidulans and subsequently have been extended to commercially important fungi. This is followed by a chapter which provides a compilation of genes isolated from commercial fungi and their present status with respect to structure, function and regulation. Chapters 3 and 4 highlight the degradative powers of filamentous fungi. First, a discussion of what is known regarding the molecular genetics of fungi and the genes and enzymes involved in the beverage and food industries. This has an oriental flavour, reflecting the tremendous importance of fungi in traditional Chinese and Japanese food production. An account of lignocellulose degradation by filamentous fungi follows, illustrating the potential of fungi to utilize this substance as a renewable energy source. The ability of fungi to produce high-value foreign proteins is reviewed in chapters 5 and 6. Chymosin production, in particular, represents a good example of high-level yields being obtained, such as to warrant commercial production.
On 800 pages this textbook provides students and professionals in life sciences, pharmacy and biochemistry with a very detailed introduction to molecular and cell biology, including standard techniques, key topics, and biotechnology in industry.
Textbook of Molecular Biotechnology covers an amazing range of topics from the basic structure of the cell and diversity of microorganisms to the latest techniques in the field of biotechnology. Various topics have been included for the benefit of graduate and postgraduate students. In addition, the book will be of immense help for the researchers and can be used as a laboratory manual for various biotechnological techniques. A number of reputed subject experts, scientists, academicians, and researchers have contributed their chapters to this volume. This book describes the role of basic biotechnological tools in various spheres of human society, namely, agriculture, nutraceuticals, pharmaceuticals, nanobiotechnology, proteomics, metagenomics and Intellectual Property rights.
The only textbook of its kind on the market, Molecular Biotechnology provides a holistic, comprehensive view of molecular biotechnology that makes it ideally suited for undergraduate majors in molecular biotechnology and biomedical sciences. Beginning with the background of this rapidly expanding field, Molecular Biotechnology covers major discoveries, regulation of the biotechnology industry, and significant innovations. A strong emphasis on careers in molecular biotechnology, profiles of major projects and researchers, and expansive discussions of bioethical concerns and current research, all come together to make this text an engaging and highly relevant resource for biotechnology students.
The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.
A study of recent developments in molecular biology and biotechnology, including enzyme technology, genetics and various applications, for example in fermentation technology, protein technology, genetic engineering and product recovery.
Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts