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Written by leading international experts in the field of plant metabolic engineering, this book discusses how the technology can be applied. Applications resulting from metabolic engineering are expected to play a very important role in the future of plant breeding: for example, in the fields of improved resistance or improved traits concerning health promoting constituents, as well as in the production of fine chemicals such as medicines, flavors and fragrances.
This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant and microbial systems; and omics technologies, such as transcriptomics, proteomics, metabolomics, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Metabolic Engineering: Methods and Protocols is a valuable resource for biologists, chemists, biotechnologists, students, and broad cohorts of researchers who works in the fields of plant metabolism and metabolic engineering.
Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.
This book presents detailed practical information on important methods used in the engineering of plant secondary metabolism pathways and the acquisition of essential knowledge in performing this activity, including important advances and emerging strategies.
Papers from a January 1997 conference held at St. Hugh's College, Oxford, review progress in the area of primary plant metabolism, and highlight the extent to which molecular techniques now influence the investigation and understanding of plant metabolism. Emphasis is centered on processes related to dominant pathways of carbohydrate production and utilization, and material is arranged to reflect the current focus of researchers on three areas of investigation: molecular architecture of selected enzymes of primary metabolism; integration of metabolism between organelles, cells, tissues, and organs; and manipulation of major pathways of carbohydrate metabolism. Annotation copyrighted by Book News, Inc., Portland, OR
Remarkable research has yielded whole genome data in plants, resulting in the documentation of an ever-increasing number of genes, without establishing their functions. The huge data resources available at the genome, transcriptome, proteome and metabolome levels are of enormous value in the field of functional genomics. This book provides insights into interpreting the sea of data in order to understand basic and practical aspects of plant metabolic engineering. It discusses in detail ways to tap into this enormous pool of data to increase productivity, and offers information that is both interesting and necessary for exploring the manipulation of metabolic pathways. The interdisciplinary approaches presented here also serve as a source of ideas for practical applications.
Plant secondary metabolism is an economically important source of fine chemicals, such as drugs, insecticides, dyes, flavours, and fragrances. Moreover, important traits of plants such as taste, flavour, smell, colour, or resistance against pests and diseases are also related to secondary metabolites. The genetic modification of plants is feasible nowadays. What does the possibility of engineering plant secondary metabolite pathways mean? In this book, firstly a general introduction is given on plant secondary metabolism, followed by an overview of the possible approaches that could be used to alter secondary metabolite pathways. In a series of chapters from various authorities in the field, an overview is given of the state of the art for important groups of secondary metabolites. No books have been published on this topic so far. This book will thus be a unique source of information for all those involved with plants as chemical factories of fine chemicals and those involved with the quality of food and ornamental plants. It will be useful in teaching graduate courses in the field of metabolic engineering in plants.
Various plant metabolites are useful for human life, and the induction and reduction of these metabolites using modern biotechnical technique is of enormous potential important especially in the fields of agriculture and health. Plant Metabolism and Biotechnology describes the biosynthetic pathways of plant metabolites, their function in plants, and some applications for biotechnology. Topics covered include: biosynthesis and metabolism of starch and sugars lipid biosynthesis symbiotic nitrogen fixation sulfur metabolism nucleotide metabolism purine alkaloid metabolism nicotine biosynthesis terpenoid biosynthesis benzylisoquinoline alkaloid biosynthesis monoterpenoid indole alkaloid biosynthesis flavonoid biosynthesis pigment biosynthesis: anthocyanins, betacyanins and carotenoids metabolomics in biotechnology Plant Metabolism and Biotechnology is an essential guide to this important field for researchers and students of biochemistry, plant biology, metabolic engineering, biotechnology, food science, agriculture, and medicine.
Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.
Metabolic and cellular engineering, as presented in this book, is a powerful alliance of two technologies: genetics-molecular biology and fermentation technology. Both are driven by continuous refinement of the basic understanding of metabolism, physiology and cellular biology (growth, division, differentiation), as well as the development of new mathematical modeling techniques. The authors' approach is original in that it integrates several disciplines into a coordinated scheme, i.e. microbial physiology and bioenergetics, thermodynamics and enzyme kinetics, biomathematics and biochemistry, genetics and molecular biology. Thus, it is called a transdisciplinary approach (TDA). The TDA provides the basis for the rational design of microorganisms or cells in a way that has rarely been utilized to its full extent.