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Gretchen L. Humason began her study of zoology and histological procedures in the 1920's. Passing away in 1991, Humason left behind over half a century of research study and instruction. Now, true to the traditions of Humason and based on her philosophies, expertise, and recommendations; Janice K. Presnell and Martin Schreibman bring us the newly-revised 5th edition of Humason's literary contributions to the theory and operation of histotechnology. Drawing on her life's work, Humason's Animal Tissue Techniques presents basic and standard tissue study procedures as well as specialized histological techniques. Designed as a guide to the principles and methods involved in histotechnology, the text begins with an overview the history of microtechnology and laboratory safety. It then proceeds to review basic procedures such as fixation, dehydration, and staining methods for specific tissue elements. Also discussed are special procedures and considerations: histochemistry, immunohistochemical methods, microwave histology, sex chromatin and chromosomes, preparation of invertebrate whole mounts and sections, and much more. This plus a section on solution preparation and general laboratory aids ensure this book will meet the diverse needs of premedical and zoology students, medical technicians, and research assistants.
This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Small Animal Soft Tissue Surgery Small Animal Soft Tissue Surgery is a comprehensive, in-depth resource for well-referenced, current information on small animal soft tissue surgery. Offering detailed surgical techniques in a well-illustrated, easy-to-follow format, the book covers the full range of surgically treated diseases and syndromes, with video clips and slideshows to demonstrate surgical procedures on an accompanying website. Chapters are written by the leading experts in surgery, internal medicine, radiology, and critical care, presenting information based on a review of the most recent literature. Divided into chapters by body system, each section begins with a brief review of the anatomy and physiology, with pathophysiology, diagnosis, treatment, and prognosis described in detail. Original illustrations and clinical photographs accompany the description of surgical techniques, and an evidence-based approach is incorporated throughout. Small Animal Soft Tissue Surgery is an essential reference for small animal surgeons, residents, and practitioners performing soft tissue surgery. KEY FEATURES Offers a detailed, evidence-based reference entirely focused on small animal soft tissue surgery Relies on the very latest literature to present the current state of the art Draws on information from surgery, internal medicine, radiology, and critical care, with contributions from experts in each area Provides detailed surgical techniques with specially commissioned, high quality line drawings Includes a website with video clips and slideshows demonstrating soft tissue procedures.
This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
Scientific experiments using animals have contributed significantly to the improvement of human health. Animal experiments were crucial to the conquest of polio, for example, and they will undoubtedly be one of the keystones in AIDS research. However, some persons believe that the cost to the animals is often high. Authored by a committee of experts from various fields, this book discusses the benefits that have resulted from animal research, the scope of animal research today, the concerns of advocates of animal welfare, and the prospects for finding alternatives to animal use. The authors conclude with specific recommendations for more consistent government action.