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Written by one of the most prominent thinkers in sound studies, Amplifications presents a perspective on sound narrated through the experiences of a sound artist and writer. A work of reflective philosophy, Amplifications sits at the intersection of history, creative practice, and sound studies, recounting this narrative through a series of themes (rattles, echoes, recordings, etc.). Carter offers a unique perspective on migratory poetics, bringing together his own compositions and life's works while using his personal narrative to frame larger theoretical questions about sound and migration.
Serves as a comprehensive review to the substantial impact of gene amplification in molecular biology, genetic engineering and medical science. The book covers the mechanism of gene amplification, organization and structure of amplified genes.
Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
This work covers the aetiology, symptoms and treatment of cancer in children, featuring contributions from leading experts on the subject. The edition includes several new chapters on future trends in chemotherapy, surgery, pallitive care and myeloid leukaemia.
Reprint of the original, first published in 1882.
Human Cholinesterases and Anticholinesterases summarizes the many fundamental concepts of human cholinesterases (ChEs) and their inhibitors. This book is divided into three chapters that represent human cholinesterase research studies from different points of view. Chapter 1 deals with the biochemical and biological background of human ChEs. This chapter also examines the molecular polymorphism, genomic origin, genetic variants, and biological roles of these enzymes. Chapter 2 provides the molecular characterization of human ChE genes and their multileveled regulation, with a special emphasis on butyrylcholinesterase. Chapter 3 discusses the scientific, ecological, and clinical implications of the studies presented in the previous chapters. Protein scientists, engineers, and researchers will find this work invaluable.
Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!