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Exciting results, perspectives, guidelines and policy recommendations for eDNA monitoring and more...
Protocols for all eDNA monitoring steps! The present protocols are state-of-the-art today, but due to continuous revisions and technical improvements, they will be optimized in the near future.
Most of the world’s mountains are rich in water and, as such, play a pivotal role in the global water cycle. They provide water for diverse human uses and ecosystems. Growing water demands as well as climate change will lead to ever-increasing pressure on mountain waters. Overcoming water-use conflicts and maintaining the ecological functioning of mountain waters presents a highly challenging task and is indispensable for sustainable development. This book extensively portrays the highly diverse attributes of mountain waters and demonstrates their paramount importance for ecological and societal development. The extensive summaries on the scientific basics of mountain waters are supplemented with considerations on the diverse water uses, needs for management actions, and challenges regarding sustainable water management. This overview concerns not only the mountain areas themselves but also downriver reaches and their surrounding lowlands, and, therefore, the relationship between mountain and lowland water issues.
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Most of the world’s mountains are rich in water and, as such, play a pivotal role in the global water cycle. They provide water for diverse human uses and ecosystems. Growing water demands as well as climate change will lead to ever-increasing pressure on mountain waters. Overcoming water-use conflicts and maintaining the ecological functioning of mountain waters presents a highly challenging task and is indispensable for sustainable development. This book extensively portrays the highly diverse attributes of mountain waters and demonstrates their paramount importance for ecological and societal development. The extensive summaries on the scientific basics of mountain waters are supplemented with considerations on the diverse water uses, needs for management actions, and challenges regarding sustainable water management. This overview concerns not only the mountain areas themselves but also downriver reaches and their surrounding lowlands, and, therefore, the relationship between mountain and lowland water issues.
We present guidelines for using environmental DNA metabarcoding, together with conventional techniques, to monitor the taxonomic diversity of phytoplankton in marine and brackish waters. The focus is on eukaryotic and prokaryotic phytoplankton using 18S and 16S rRNA primers and high-throughput sequencing. Information and recommendations on sampling, sample processing, molecular biological work, quality control, bioinformatics, data storage and management and cost estimates are included so that the method can be used to complement standardized light microscopy. A scientific literature review, discussion on future perspectives, reference databases and standardization are included. Using eDNA metabarcoding to complement standardized light microscopy advances conventional monitoring and research on phytoplankton communities to assess biodiversity and the state of the marine environment.
100 hikes in the Alpine Lakes Wilderness, including the newly protected Middle Fork Snoqualmie Valley Mix of day hikes and classic backpacking routes Stunning, oversized full-color guide The lush Alpine Lakes Wilderness in Washington’s Central Cascades contains a plethora of trails, rugged glacier-carved mountains, and more than 700 sparkling alpine lakes and ponds. Accessed via nearly 50 trailheads, more than 600 miles of trails offer hikers leisurely strolls along wooded creeks, climbs up mountain passes, or lunch spots next to glassy tarns. It is one of the most popular and beloved places for hikers in this region. The all-new guide, Alpine Lakes Wilderness, features a wide range of hikes that vary in difficulty, geography, and theme so that hikers of any age and skill level will find trails that fit their taste. Easy to use, the guide includes details on overnight permits, car-camping options near wilderness access points, detailed maps, elevation gain/ loss, and turn-by-turn mileage and directions. Interesting historical background and natural history round out the trail descriptions.