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Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. - Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods - Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more - Clear, straightforward explanations of each technique for anyone new to the field - A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture - Detailed recommendations on where to find protocols and other resources for specific techniques - "Walk-through" boxes that guide readers through experiments step-by-step
Historically the search for the neural bases of behavior goes back a long way. Neuroethology, which is concerned with the experimental analysis ofthe releasing and control mech anisms of behavior, is a young discipline. Results from this multidisciplinary branch of research, which uses physical, chemical, and mathematical methods, have not yet been extensively treated in textbooks of neurophysiology and ethology. This book is intended as a first attempt to pose major questions of neuroethology and to demonstrate, by means of selected research examples, some of the ways by which these questions are being approached. Inevitably this cannot be a complete and in depth detailed treatment of all of the neurobiology examples, and I realize that such a selection is of a subjective nature. The overall goal ofthe book is to present an introduction. After outlining some of the very basic neurophysiological and ethological concepts (Chaps. 2 and 3), neuroethological questions and methods are demonstrated extensively by means of a particular example (Chap. 4). There are two reasons to choose the visually guided prey-catching and avoidance behavior of the Common Toad: (1) it is a system which I have inves tigated for about fifteen years and therefore know best, (2) the toad story is one of the most comprehensive neuro ethological approaches so far. Thus, it is possible here to outline the major concepts of neuroethology and to pose the basic questions.
Vocal signals are central for social communication across a wide range of vertebrate species; consequently, it is critical to understand the mechanisms underlying the learning, control, and evolution of vocal communication. Songbirds are at the forefront of research into such neural mechanisms. Indeed, songbirds provide a particularly important model system for this endeavor because of the many parallels between birdsong and human speech. Specifically, (1) songbirds are one of the few vertebrate species that, like humans, learn their vocal signals during development, (2) the processes of song learning and control in songbirds shares many parallels with the process of speech acquisition in humans, and (3) there exist deep homologies between the circuits for the learning, control, and processing of vocal signals across songbirds and humans. In addition, because of the diversity of songbirds and song learning strategies, songbirds offer a powerful model system to use the comparative method to reveal mechanisms underlying the evolution of song learning and production. Taken together, research on songbirds can not only reveal general principles underlying vertebrate vocal communication but can also provide insight into potential mechanisms underlying the learning, control, and processing of speech. This volume will cover a range of topics in birdsong spanning multiple level of analysis. Chapters will be authored by the world’s leading experts on birdsong and will provide comprehensive reviews of the processes underlying song learning, of the neural circuits for song learning and control as well as for the extraction and processing of song information, of the selection pressures underlying song evolution, and of genetic and molecular mechanisms underlying the learning and evolution of song. The primary goals of this volume are to provide comprehensive, integrative, and comparative perspectives on birdsong and to underscore the importance of birdsong to biomedical research, evolutionary biology, and behavioral, systems, and computational neuroscience.The target audience of this volume will be graduate students, postdoctoral fellows, and established academics and neuroscientists who are interested in mechanisms of communication from an integrative and comparative perspective. The volume is intended to function as a high-profile and contemporary reference on current work related to the learning, control, processing, and evolution of birdsong. This volume will have broad appeal to comparative and sensory biologists, neurophysiologists, and behavioral, systems, and cognitive neuroscientists who attend meetings such as the Society for Neuroscience, the International Society for Neuroethology, and the Society for Integrative and Comparative Biology. Because of the relevance of birdsong research to understanding human speech, it is likely that the volume will also be of interest to speech researchers and clinicians researching communication, motor, and sensory processing disorders.
This volume covers the current status of research in the neurobiology of motivated behaviors in humans and other animals in healthy condition. This includes consideration of the psychological processes that drive motivated behavior and the anatomical, electrophysiological and neurochemical mechanisms which drive these processes and regulate behavioural output. The volume also includes chapters on pathological disturbances in motivation including apathy, or motivational deficit as well as addictions, the pathological misdirection of motivated behavior. As with the chapters on healthy motivational processes, the chapters on disease provide a comprehensive up to date review of the neurobiological abnormalities that underlie motivation, as determined by studies of patient populations as well as animal models of disease. The book closes with a section on recent developments in treatments for motivational disorders.
The world of crickets has long been a world of scientific adventure and human fascination. Because of their remarkable ways of communicating and because their nervous and endocrine systems are easily accessible to researchers, crickets can be studied and analyzed with great effectiveness. Starting in the 1960s, vastly improved behavioral and neurobiological techniques have brought them to the frontier of the new field of neuroethology. Here, in the most comprehensive book on crickets ever compiled, twenty-five leading scientists detail the present state of cricket research both at conceptual and at experimental levels. They tell about the manifold strategies crickets use in matching development with seasons and habitats, finding mates, and avoiding parasites and predators, and they describe the physiological mechanisms, especially the neuronal mechanisms, underlying cricket behavior. Their book is at once about communication, comparative physiology and anatomy, and environmental interaction. More than half of Cricket Behavior and Neurobiology is devoted to acoustic behavior and bioacoustics. It is intended for those interested in entomology, general and comparative physiology, biophysics, endocrinology, and chronobiology. It offers new information for behavioral physiologists and ecologists, bioacousticians, and especially neurobiologists concerned with behavior.
This volume was generated from papers presented at the Second Triennial Symposium of the International Basal Ganglia Society (IBAGS) held at the University of Victoria, British Columbia, July 21-23, 1986. The meeting was held as a satellite symposium following the XXX Congress of the International Union of Physiological Sciences at Vancouver. IBAGS was founded at a similar satellite symposium held in Lorne, Australia, organized by John S. McKenzie and sponsored by the University of Melbourne. The symposium held in Australia was attended by 50 scientists from 12 different countries. The results of the first symposium, edited by John S. McKenzie, Robert E. Kemm and Lynette N. Wilcock, were published by Plenum Press in 1984 under the title, The Basal Ganglia - Structure and Function. It was decided that the Society should meet on a triennial basis. The time and place for Second IBAGS Symposium were set by A.G. Phillips who served as Chairman of the Program Committee along with I. Divac, S.A. Greenfield and E.T. Rolls and J.S. McKenzie. Michael E. Corcoran of the Department of Psychology, University of Victoria served as the on-site coordinator and arranger for the Symposium. He was ably assisted by Ms. Morag McNeil who handled the details which made the meeting run smoothly.
First multi-year cumulation covers six years: 1965-70.