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Well-established model systems exist in four out of the seven major classes of vertebrates. These include the mouse, chicken, frog and zebrafish. Noticeably missing from this list is a reptilian model organism for comparative studies between the vertebrates and for studies of biological processes unique to reptiles. To help fill in this gap the green anole lizard, Anolis carolinensis, is being adapted as a model organism. Despite the recent release of the complete genomic sequence of the A. carolinensis, the lizard lacks some resources to aid researchers in their studies. Particularly, the lack of transcriptomic resources for lizard has made it difficult to identify genes complete with alternative splice forms and untranslated regions (UTRs). As part of this work the genome annotation for A. carolinensis was improved through next generation sequencing and assembly of the transcriptomes from 14 different adult and embryonic tissues. This revised annotation of the lizard will improve comparative studies between vertebrates, as well as studies within A. carolinensis itself, by providing more accurate gene models, which provide the bases for molecular studies. To demonstrate the utility of the improved annotations and reptilian model organism, the developmental process of somitogenesis in the lizard was analyzed and compared with other vertebrates. This study identified several key features both divergent and convergent between the vertebrates, which was not previously known before analysis of a reptilian model organism. The improved genome annotations have also allowed for molecular studies of tail regeneration in the lizard. With the annotation of 3' UTR sequences and next generation sequencing, it is now possible to do expressional studies of miRNA and predict their mRNA target transcripts at genomic scale. Through next generation small RNA sequencing and subsequent analysis, several differentially expressed miRNAs were identified in the regenerating tail, suggesting miRNA may play a key role in regulating this process in lizards. Through miRNA target prediction several key biological pathways were identified as potentially under the regulation of miRNAs during tail regeneration. In total, this work has both helped advance A. carolinensis as model system and displayed the utility of a reptilian model system.
"In a book both beautifully illustrated and deeply informative, Jonathan Losos, a leader in evolutionary ecology, celebrates and analyzes the diversity of the natural world that the fascinating anoline lizards epitomize. Readers who are drawn to nature by its beauty or its intellectual challenges—or both—will find his book rewarding."—Douglas J. Futuyma, State University of New York, Stony Brook "This book is destined to become a classic. It is scholarly, informative, stimulating, and highly readable, and will inspire a generation of students."—Peter R. Grant, author of How and Why Species Multiply: The Radiation of Darwin's Finches "Anoline lizards experienced a spectacular adaptive radiation in the dynamic landscape of the Caribbean islands. The radiation has extended over a long period of time and has featured separate radiations on the larger islands. Losos, the leading active student of these lizards, presents an integrated and synthetic overview, summarizing the enormous and multidimensional research literature. This engaging book makes a wonderful example of an adaptive radiation accessible to all, and the lavish illustrations, especially the photographs, make the anoles come alive in one's mind."—David Wake, University of California, Berkeley "This magnificent book is a celebration and synthesis of one of the most eventful adaptive radiations known. With disarming prose and personal narrative Jonathan Losos shows how an obsession, beginning at age ten, became a methodology and a research plan that, together with studies by colleagues and predecessors, culminated in many of the principles we now regard as true about the origins and maintenance of biodiversity. This work combines rigorous analysis and glorious natural history in a unique volume that stands with books by the Grants on Darwin's finches among the most informed and engaging accounts ever written on the evolution of a group of organisms in nature."—Dolph Schluter, author of The Ecology of Adaptive Radiation
The sequencing of the green anole lizard (Anolis carolinensis ) genome has already provided insights into how vertebrate genomes have evolved since the phylogenetic split between reptiles and mammals ∼300 million years ago. For instance, the diversity and abundance transposable elements (TEs) in the Anolis genome, particularly the non-LTR retrotransposons (nLTR-LTs), shows more similarity to fish than to mammals, which suggests that mammals have significantly diverged from the amniote ancestor in terms of genome structure. The fate of TEs in a genome relies on the relative strengths of purifying selection against deleterious elements and genetic drift in host populations. Surprisingly, the geographic distribution and demographic history of populations within A. carolinensis has largely escaped scrutiny. We studied the patterns of mitochondrial and nuclear DNA sequences and found that there are five evolutionary lineages of green anoles, which diverged ∼2 million years ago. Climatic shifts during the early Pleistocene may have driven their early diversification, particularly on ancient island refugia in what is now Florida, where a remarkable phylogeographic diversity of green anoles is found. More recently, the dispersal of green anoles onto the continental mainland led to a dramatic westward range expansion across the Gulf Coastal Plain. These insights into the evolutionary history of A. carolinensis allowed us to infer the population dynamics of nLTR-RTs in the Anolis genome. While nLTR-RTs are rare in Anolis, we find that they reach fixation in populations quite readily. We also find that full-length (FL) nLTR-RT insertions may be subjected to purifying selection as they are found at lower population frequencies than truncated (TR) insertions. This suggests deleterious effects of ectopic recombination or the process of retrotransposition are limiting the copy number of FL elements in Anolis. Finally, we find that FL elements are much more likely to be fixed in populations of small effective size, where purifying selection may not be acting as efficiently due to strong genetic drift. While FL elements are subjected to purifying selection, the fixation of TR elements suggests that another mechanism, such as DNA loss, may account for the relative paucity of nLTR-RTs in the Anolis genome.
The Anolis lizards of the Caribbean are ideally suited for the study of evolutionary ecology. Offering fascinating insights into the more than 150 species dispersed throughout the Caribbean islands, Jonathan Roughgarden details the differences between species in a wide range of behavioral and physical characteristics, including foraging behaviors, body size, and habitat use, resulting from evolutionary divergences concurrent with the plate-tectonic origins of the region. This book will be of interest to students and researchers--ecology and theoretical, tropical, and population biology.
The Anolis lizards of the Caribbean are ideally suited for the study of evolutionary ecology. Offering fascinating insights into the more than 150 species dispersed throughout the Caribbean islands, Jonathan Roughgarden details the differences between species in a wide range of behavioral and physical characteristics, including foraging behaviors, body size, and habitat use, resulting from evolutionary divergences concurrent with the plate-tectonic origins of the region. This book will be of interest to students and researchers--ecology and theoretical, tropical, and population biology.
The present review covers a very neglected field in regeneration studies, namely, tissue and organ regeneration in reptiles, especially represented by the lizard model of regeneration. The term “regeneration” is intended here as “the ability of an adult organism to recover damaged or completely lost body parts or organs.” The process of recovery is further termed “restitutive regeneration” when the lost part is reformed and capable of performing the complete or partial physiological activity performed by the original, lost body part. Lizards represent the only amniotes that at the same time show successful organ regeneration, in the tail, and organ failure, in the limb (Marcucci 1930a, b; Simpson 1961, 1970, 1983). This condition offers a unique opportunity to study at the same time mechanisms that in different regions of the same animal control the success or failure of regeneration. The lizard model is usually neglected in the literature despite the fact that the lizard is an amniote with a basic histological structure similar to that of mammals, and it is therefore a better model than the salamander (an a- mniote) model to investigate regeneration issues.
Key features: Presents a contemporary snapshot of the mechanisms underlying the evolution and adaptation of behavior Explores how genetics, epigenetics, development, and environment shape behavior Discusses a broad range of behavioral repertoires and responses, including those related to thermoregulatory, foraging, predatory, displaying, social and escape strategies. Examines physiological and sensory mechanisms Covers the effects of various aspects of global change on behavior, with chapters that focus on the impacts of climate change on hydroregulatory behavior and behavioral responses to the effects of habitat alteration resulting from human-mediated change and colonization by invasive species. Lizards serve as focal organisms for many of biological questions related to evolution, ecology, physiology, and morphology. They are studied at multiple spatial and temporal scales, from the individual to the community level. This book, authored by expert contributors from around the world, explores behaviors underlying the evolution and adaptation of these organisms. It covers conceptual, empirical, and methodological approaches to the understanding of the role that natural and sexual selection play in molding the behavioral traits of lizards. This thorough, illustrated reference should stimulate discussion of the conceptual and methodological approaches for studying the behavioral traits of these fascinating and highly diverse vertebrates.
Annelids offer a diversity of experimentally accessible features making them a rich experimental subject across the biological sciences, including evolutionary development, neurosciences and stem cell research. This volume introduces the Annelids and their utility in evolutionary developmental biology, neurobiology, and environmental/ecological studies, including extreme environments. The book demonstrates the variety of fields in which Annelids are already proving to be a useful experimental system. Describing the utility of Annelids as a research model, this book is an invaluable resource for all researchers in the field.
This book provides an overview of the diversity of lizards and their major adaptive features. The authors discuss the latest research findings and provide new hypotheses about lizard diversity.