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cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods that determine the structures of biological molecules, a vital step for understanding their function - Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted
Enzymes a revised frequently in modifying proteins for specialized uses. These books cover the latest advances in this field and its applications in the field of molecular biology.
Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.
Approx.504 pagesApprox.504 pages
This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art. Directed molecular evolution has become the most reliable and robust method to tailor enzymes, metabolic pathways or even whole microorganisms with improved traits. By mirroring the Darwinian algorithm of natural selection on a laboratory scale, new biomolecules of invaluable biotechnological interest can now be engineered in a manner that surpasses the boundaries of nature. The volume is divided into two sections, the first of which provides an update on recent successful cases of enzyme ensembles from different areas of the biotechnological spectrum, including tryptophan synthases, unspecific peroxygenases, phytases, therapeutic enzymes, stereoselective enzymes and CO2-fixing enzymes. This section also provides information on the directed evolution of whole cells. The second section of the book summarizes a variety of the most applicable methods for library creation, together with the future trends aimed at bringing together directed evolution and in silico/computational enzyme design and ancestral resurrection.
Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.
Advances in Enzymology and Related Areas of Molecular Biology is a seminal series in the field of biochemistry, offering researchers access to authoritative reviews of the latest discoveries in all areas of enzymology and molecular biology. These landmark volumes date back to 1941, providing an unrivaled view of the historical development of enzymology. The series offers researchers the latest understanding of enzymes, their mechanisms, reactions and evolution, roles in complex biological process, and their application in both the laboratory and industry. Each volume in the series features contributions by leading pioneers and investigators in the field from around the world. All articles are carefully edited to ensure thoroughness, quality, and readability. With its wide range of topics and long historical pedigree, Advances in Enzymology and Related Areas of Molecular Biology can be used not only by students and researchers in molecular biology, biochemistry, and enzymology, but also by any scientist interested in the discovery of an enzyme, its properties, and its applications.
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Enzyme Regulation in Metabolic Pathways shows the reader how to understand the roles of enzymes and their kinetic constants in intermediary metabolism. It provides a means of correlating data obtained in experimental studies to multiple possible mechanisms through which some enzyme may catalyze the conversion of a substrate to a product. Although not the most appropriate means of determining some potential kinetic mechanism, quasi-equilibrium assumptions are used throughout the book, keeping the rate equation derivations simple. Actual metabolic pathways with known (presumed) positive and negative regulation events are linked to these potential kinetic mechanisms using both rate equation derivations and data plots illustrating how the rate equation derivations can be used to explain the data plots. This book will be a valuable reference for students in biological sciences and biochemistry majors required to take a core course in enzymology.