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This book will highlight advanced techniques that were recently used for studying microorganisms in extreme environments. Recent technological leaps in the study of microorganisms in the environment now make it possible to comprehensively study microbes in the environment. Extreme environments could benefit from the application of these techniques, but many challenges such as low biomass, low activity and slow growth has prevented their wide adoption. This book will review recent application of state-of-the-art techniques in extreme environments, helping researcher and graduate students get a better knowledge of the tools available.
In 1898, an Austrian microbiologist Heinrich Winterberg made a curious observation: the number of microbial cells in his samples did not match the number of colonies formed on nutrient media (Winterberg 1898). About a decade later, J. Amann qu- tified this mismatch, which turned out to be surprisingly large, with non-growing cells outnumbering the cultivable ones almost 150 times (Amann 1911). These papers signify some of the earliest steps towards the discovery of an important phenomenon known today as the Great Plate Count Anomaly (Staley and Konopka 1985). Note how early in the history of microbiology these steps were taken. Detecting the Anomaly almost certainly required the Plate. If so, then the period from 1881 to 1887, the years when Robert Koch and Petri introduced their key inventions (Koch 1881; Petri 1887), sets the earliest boundary for the discovery, which is remarkably close to the 1898 observations by H. Winterberg. Celebrating its 111th anniversary, the Great Plate Count Anomaly today is arguably the oldest unresolved microbiological phenomenon. In the years to follow, the Anomaly was repeatedly confirmed by all microb- logists who cared to compare the cell count in the inoculum to the colony count in the Petri dish (cf., Cholodny 1929; Butkevich 1932; Butkevich and Butkevich 1936). By mid-century, the remarkable difference between the two counts became a universally recognized phenomenon, acknowledged by several classics of the time (Waksman and Hotchkiss 1937; ZoBell 1946; Jannasch and Jones 1959).
A diverse account of how life exists in extreme environments and these systems' susceptibility and resilience to climate change.
This book provides a broad overview how extremophiles can be used in biotechnology, including for the production and degradation of compounds. It reviews various recent discoveries and applications related to a large variety of extremophiles, considering both prokaryotes as well as eukaryotes.
People's desire to understand the environments in which they live is a natural one. People spend most of their time in spaces and structures designed, built, and managed by humans, and it is estimated that people in developed countries now spend 90 percent of their lives indoors. As people move from homes to workplaces, traveling in cars and on transit systems, microorganisms are continually with and around them. The human-associated microbes that are shed, along with the human behaviors that affect their transport and removal, make significant contributions to the diversity of the indoor microbiome. The characteristics of "healthy" indoor environments cannot yet be defined, nor do microbial, clinical, and building researchers yet understand how to modify features of indoor environmentsâ€"such as building ventilation systems and the chemistry of building materialsâ€"in ways that would have predictable impacts on microbial communities to promote health and prevent disease. The factors that affect the environments within buildings, the ways in which building characteristics influence the composition and function of indoor microbial communities, and the ways in which these microbial communities relate to human health and well-being are extraordinarily complex and can be explored only as a dynamic, interconnected ecosystem by engaging the fields of microbial biology and ecology, chemistry, building science, and human physiology. This report reviews what is known about the intersection of these disciplines, and how new tools may facilitate advances in understanding the ecosystem of built environments, indoor microbiomes, and effects on human health and well-being. It offers a research agenda to generate the information needed so that stakeholders with an interest in understanding the impacts of built environments will be able to make more informed decisions.
The cryosphere stands for environments where water appears in a frozen form. It includes permafrost, glaciers, ice sheets, and sea ice and is currently more affected by Global Change than most other regions of the Earth. In the cryosphere, limited water availability and subzero temperatures cause extreme conditions for all kind of life which microorganisms can cope with extremely well. The cryosphere’s microbiota displays an unexpectedly large genetic potential, and taxonomic as well as functional diversity which, however, we still only begin to map. Also, microbial communities influence reaction patterns of the cryosphere towards Global Change. Altered patterns of seasonal temperature fluctuations and precipitation are expected in the Arctic and will affect the microbial turnover of soil organic matter (SOM). Activation of nutrients by thawing and increased active layer thickness as well as erosion renders nutrient stocks accessible to microbial activities. Also, glacier melt and retreat stimulate microbial life in turn influencing albedo and surface temperatures. In this context, the functional resilience of microbial communities in the cryosphere is of major interest. Particularly important is the ability of microorganisms and microbial communities to respond to changes in their surroundings by intracellular regulation and population shifts within functional niches, respectively. Research on microbial life exposed to permanent freeze or seasonal freeze-thaw cycles has led to astonishing findings about microbial versatility, adaptation, and diversity. Microorganisms thrive in cold habitats and new sequencing techniques have produced large amounts of genomic, metagenomic, and metatranscriptomic data that allow insights into the fascinating microbial ecology and physiology at low and subzero temperatures. Moreover, some of the frozen ecosystems such as permafrost constitute major global carbon and nitrogen storages, but can also act as sources of the greenhouse gases methane and nitrous oxide. In this book we summarize state of the art knowledge on whether environmental changes are met by a flexible microbial community retaining its function, or if the altered conditions also render the community in a state of altered properties that affect the Earth’s element cycles and climate. This book brings together research on the cryosphere’s microbiota including permafrost, glaciers, and sea ice in Arctic and Antarctic regions. Different spatial scales and levels of complexity are considered, spanning from ecosystem level to pure culture studies of model microbes in the laboratory. It aims to attract a wide range of parties with interest in the effect of climate change and/or low temperatures on microbial nutrient cycling and physiology.
This book summarizes the basics of actinobacteria, from microbiology to synthetic biology. It focuses on diversity, NRPS, sesquiterpenes, lantipeptide, bioinformatics apparatuses, cloning, CRISPR, reverse engineering, FDA supported medications, and marine actinobacteria. It also covers the latest trends in drug discovery from actinobacteria, and introduces several recently developed bioinformatics and synthetic biology tools to explore new antibiotics from actinobacteria. Many natural products such as polyketides, isoprenoids, phenazines, peptides, indolocarbarbazoles, sterols, and others have been isolated and characterized from actinobacteria. Some products are synthesized by the non-ribosomal peptide synthetases (NRPSs), polyketide synthases (PKSs), or other functional genes. Although genome sequencing has uncovered the differing qualities of these chemicals, recognizing new items and their biosynthetic pathways is still under examination. Cryptic metabolic pathways have been explored using molecular techniques or culture-dependent approaches. In recent years, researchers’ primary interest is to identify the specific conditions or agents that wake the cryptic antibiotics. Several bioinformatics and synthetic biology tools were developed to explore new antibiotics from actinobacteria. The book comprises 14 chapters with different aspects of application and utilization of actinomycetes from the microbiology; systems biology, pharmacology of natural products, bioinformatics, actinomycete and its diversity, CRISPR, artificial Intelligence, synthetic biology, metabolic engineering, expressional studies, and biosynthetic gene clusters. The book delivers useful information on actinomyces to researchers, novices in genome designing, specialists, clinicians, policymakers, and professionals.
Rock surfaces provide a challenging habitat for a broad diversity of micro- or small-sized organisms. They interact with each other forming complex communities as well with their substrate causing biodeterioration of rock. Extreme fluctuation in light, temperature and hydration are the main factors that determine the rock surface habitats. The habitat includes epilithic organisms which thrive on the surface without penetrating the rock, endolithic organisms which live just beneath the surface using a thin layer of the rock surface for protection against adverse conditions of the environment (e.g. light protection, storage of water) and chasmo-endolithic organisms which use fractures of the rock surface for a more habitable environment. The book will provide an overview of the various organismal groups, from prokaryotes to vascular plants and arthropods, as well as survey organism-mediated interactions with the rock surface. The latter include biogenic weathering (biogeochemistry, state-of-the art imaging methods), photosynthesis and nitrogen fixation at and inside the rock surface.
Microbiology of Atypical Environments, Volume 45, presents a comprehensive reference text on the microbiological methods used to research the basic biology of microorganism in harsh, stressful and sometimes atypical environments (e.g. arctic ice, space stations, extraterrestrial environments, hot springs and magnetic environments). Chapters in this release include Biofilms in space, Methods for studying the survival of microorganisms in extraterrestrial environments, Persistence of Fungi in Atypical (Closed) Environments Based on Evidence from the International Space Station (ISS): Distribution and Significance to Human health, Methods for visualizing microorganisms in Icy environments, Measuring microbial metabolism at surface-air interfaces and nuclear waste management, amongst others. - Contains both established and emerging methods - Provides excellent reference lists on the topics covered
From arid deserts to icy poles, outer space to the depths of the sea, this exciting new work studies the remarkable life forms that have made these inhospitable environments their home. Covering not only micro-organisms, but also higher plants and animals such as worms, fish and polar plants, this book details the ecological, biological and biogeochemical challenges these organisms face and unifying themes between environments. Equally useful for the expert, student and casual scientific reader, this book also explores the impact of climate change, rapid seasonal changes and pollution on these extraordinary creatures.