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Advance Laboratory Techniques in Zoology include Principles and applications of Centrifugation, Filtration, Microscopy (Scanning and Transmission Electron microscopes). These also include Micro-photography, Micrometry, Chromatography, Spectrophotometry, Electrophoresis (Agarose and PAGE), Southern and Northern Blotting. Advanced Techniques in Biochemistry are widely used for Extraction, Precipitation and estimation of Amino acids and Proteins, Lipids, Nucleic acids and the study of enzyme activity. Histological (sectioning and staining) and Molecular Biology techniques: Vector isolation, Gene insertion, Recombinant DNA Production, Gene amplification by PCR, DNA and vector purification, Preparation of genomic libraries and sub-cloning using plasmid vectors are widely used in Zoology. Immunological techniques like Radioimmunoassay, ELISA, Development of vaccines, Aseptic culture techniques, bacterial growth, isolation and identification of bacteria as well as Use of Statistical Methods are commonly used techniques in Zoology Research.
Laboratory Exercises in Zoology serves as a teaching aid for students studying for Advanced level Zoology or Biology. This book provides exercises concerned mainly with physiology and some dissection techniques. Organized into 12 parts, this book begins with an overview of diffusion of molecules or ions from a region of high concentration to a region of relatively low concentration. This text then discusses the breakdown of complex molecules, which is achieved by a series of hydrolyses catalyzed by the digestive enzymes produced by the glandular cells of the digestive system. Other chapters consider the various stages involved in making permanent stained preparations. This book discusses as well the requirements for animals in the laboratory. The final chapter deals with the rate of growth of an organism. This book is a valuable resource for students studying zoology and biology. Teachers and biology laboratory technicians will also find this book extremely useful.
A complete laboratory manual in which the methods of practical experimentation are adequately complemented by theoretical fundamentals. The book though primarily developed for the undergraduate course students, also, caters to the first-year postgraduate
Practical Zoology for Advanced Level and Intermediate Students is a laboratory manual that covers various zoological experiments. The book presents methods, techniques, and illustrations relevant to zoological experiments. The text first discusses microscopical techniques, and then proceeds to tackling the morphology and anatomy of various animals. Next, the book deals with cytology and histology. The next part covers elementary biochemistry. The fifth part discusses physiology, while the sixth part covers genetics. The last part deals with vertebrate embryology. The book will be most useful to students of disciplines concerned with animal biology, such as veterinary medicine and comparative anatomy.
"Lab Dynamics is a book about the challenges to doing science and dealing with the individuals involved, including oneself. The authors, a scientist and a psychotherapist, draw on principles of group and behavioral psychology but speak to scientists in their own language about their own experiences. They offer in-depth, practical advice, real-life examples, and exercises tailored to scientific and technical workplaces on topics as diverse as conflict resolution, negotiation, dealing with supervision, working with competing peers, and making the transition from academia to industry." "This is a uniquely valuable contribution to the scientific literature, on a subject of direct importance to lab heads, postdocs, and students. It is also required reading for senior staff concerned about improving efficiency and effectiveness in academic and industrial research."--BOOK JACKET
A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.
Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. Presents basic analytical protocols and sample-preparation guidelines Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research Presents biostatistical tools and methods and basic computational models in biosciences